Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and holC overlap by 1 nucleotides holC and pepA are separated by 159 nucleotides
b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860
valS
b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303
holC
b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974
pepA
Position (kb)
4481
4482
4483 Strain fitness (log2 ratio)
-2
-1
0
1 at 4482.341 kb on + strand at 4482.349 kb on - strand at 4482.354 kb on - strand at 4482.369 kb on - strand at 4482.369 kb on - strand at 4482.379 kb on - strand at 4482.394 kb on + strand at 4482.394 kb on - strand at 4482.402 kb on - strand at 4482.402 kb on - strand at 4482.404 kb on + strand at 4482.445 kb on + strand at 4482.452 kb on + strand at 4482.458 kb on + strand at 4482.460 kb on + strand at 4482.494 kb on + strand at 4482.582 kb on - strand at 4482.650 kb on + strand, within pepA at 4482.690 kb on - strand, within pepA at 4482.698 kb on - strand, within pepA at 4482.706 kb on + strand at 4482.706 kb on + strand at 4482.726 kb on - strand, within pepA at 4482.741 kb on + strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.787 kb on + strand, within pepA at 4482.787 kb on + strand, within pepA at 4482.800 kb on - strand, within pepA at 4482.805 kb on - strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.883 kb on + strand, within pepA at 4482.900 kb on + strand, within pepA at 4482.900 kb on + strand, within pepA at 4482.900 kb on + strand, within pepA at 4482.900 kb on + strand, within pepA at 4482.900 kb on + strand, within pepA at 4482.908 kb on - strand, within pepA at 4482.920 kb on + strand, within pepA at 4482.959 kb on - strand, within pepA at 4482.964 kb on - strand, within pepA at 4482.964 kb on - strand, within pepA at 4482.977 kb on + strand, within pepA at 4482.977 kb on + strand, within pepA at 4482.985 kb on - strand, within pepA at 4483.056 kb on - strand, within pepA at 4483.056 kb on - strand, within pepA at 4483.122 kb on + strand, within pepA at 4483.150 kb on + strand, within pepA at 4483.158 kb on - strand, within pepA at 4483.169 kb on - strand, within pepA at 4483.179 kb on + strand, within pepA
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 4,482,341 + -1.0 4,482,349 - -0.2 4,482,354 - +0.1 4,482,369 - +0.2 4,482,369 - +0.7 4,482,379 - -0.5 4,482,394 + -1.1 4,482,394 - +0.6 4,482,402 - +0.4 4,482,402 - -0.4 4,482,404 + -0.7 4,482,445 + +0.3 4,482,452 + -0.2 4,482,458 + -1.5 4,482,460 + -1.1 4,482,494 + -1.7 4,482,582 - -0.7 4,482,650 + pepA b4260 0.12 -0.3 4,482,690 - pepA b4260 0.15 -0.8 4,482,698 - pepA b4260 0.16 -0.5 4,482,706 + +1.1 4,482,706 + -0.3 4,482,726 - pepA b4260 0.17 -0.9 4,482,741 + pepA b4260 0.18 +0.6 4,482,749 - pepA b4260 0.19 -0.0 4,482,749 - pepA b4260 0.19 -1.6 4,482,749 - pepA b4260 0.19 +0.5 4,482,787 + pepA b4260 0.21 -1.5 4,482,787 + pepA b4260 0.21 +0.5 4,482,800 - pepA b4260 0.22 +0.2 4,482,805 - pepA b4260 0.23 +0.5 4,482,822 + pepA b4260 0.24 -0.3 4,482,822 + pepA b4260 0.24 -0.6 4,482,822 + pepA b4260 0.24 +0.5 4,482,822 + pepA b4260 0.24 +0.8 4,482,883 + pepA b4260 0.28 -0.5 4,482,900 + pepA b4260 0.29 -1.3 4,482,900 + pepA b4260 0.29 -0.5 4,482,900 + pepA b4260 0.29 -0.2 4,482,900 + pepA b4260 0.29 -0.8 4,482,900 + pepA b4260 0.29 -0.5 4,482,908 - pepA b4260 0.29 -0.1 4,482,920 + pepA b4260 0.30 -1.7 4,482,959 - pepA b4260 0.33 +0.4 4,482,964 - pepA b4260 0.33 +1.4 4,482,964 - pepA b4260 0.33 -0.9 4,482,977 + pepA b4260 0.34 +1.2 4,482,977 + pepA b4260 0.34 +0.9 4,482,985 - pepA b4260 0.35 -0.2 4,483,056 - pepA b4260 0.39 +0.4 4,483,056 - pepA b4260 0.39 -0.7 4,483,122 + pepA b4260 0.44 -0.8 4,483,150 + pepA b4260 0.45 -2.0 4,483,158 - pepA b4260 0.46 +0.2 4,483,169 - pepA b4260 0.47 +0.2 4,483,179 + pepA b4260 0.47 -1.5
Or see this region's nucleotide sequence