Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt qor and dnaB are separated by 82 nucleotides dnaB and alr are separated by 52 nucleotides
b4051: qor - quinone oxidoreductase, NADPH-dependent (NCBI), at 4,261,271 to 4,262,254
qor
b4052: dnaB - replicative DNA helicase (NCBI), at 4,262,337 to 4,263,752
dnaB
b4053: alr - alanine racemase (NCBI), at 4,263,805 to 4,264,884
alr
Position (kb)
4262
4263
4264 Strain fitness (log2 ratio)
-1
0
1 at 4261.346 kb on - strand at 4261.346 kb on - strand at 4261.358 kb on - strand at 4261.358 kb on - strand at 4261.382 kb on - strand, within qor at 4261.382 kb on - strand, within qor at 4261.394 kb on - strand, within qor at 4261.406 kb on - strand, within qor at 4261.406 kb on - strand, within qor at 4261.478 kb on - strand, within qor at 4261.587 kb on + strand, within qor at 4261.593 kb on - strand, within qor at 4261.610 kb on + strand, within qor at 4261.698 kb on - strand, within qor at 4261.698 kb on - strand, within qor at 4261.955 kb on + strand, within qor at 4261.955 kb on - strand, within qor at 4261.977 kb on + strand, within qor at 4261.984 kb on - strand, within qor at 4261.991 kb on + strand, within qor at 4262.007 kb on - strand, within qor at 4262.007 kb on - strand, within qor at 4262.007 kb on - strand, within qor at 4262.042 kb on - strand, within qor at 4262.079 kb on - strand, within qor at 4262.195 kb on - strand at 4263.790 kb on + strand at 4263.828 kb on - strand at 4263.828 kb on - strand at 4263.871 kb on - strand at 4263.919 kb on + strand, within alr at 4263.928 kb on - strand, within alr at 4264.017 kb on + strand, within alr at 4264.017 kb on + strand, within alr at 4264.102 kb on - strand, within alr at 4264.142 kb on - strand, within alr at 4264.146 kb on - strand, within alr at 4264.146 kb on - strand, within alr at 4264.220 kb on - strand, within alr at 4264.232 kb on + strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.240 kb on - strand, within alr at 4264.266 kb on + strand, within alr at 4264.406 kb on - strand, within alr at 4264.406 kb on - strand, within alr at 4264.424 kb on + strand, within alr at 4264.462 kb on + strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.525 kb on - strand, within alr at 4264.620 kb on + strand, within alr at 4264.620 kb on + strand, within alr at 4264.639 kb on - strand, within alr at 4264.658 kb on + strand, within alr at 4264.658 kb on + strand, within alr at 4264.661 kb on + strand, within alr at 4264.661 kb on + strand, within alr at 4264.661 kb on - strand, within alr at 4264.664 kb on - strand, within alr at 4264.722 kb on - strand, within alr at 4264.729 kb on + strand, within alr
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 4,261,346 - +0.4 4,261,346 - +0.3 4,261,358 - +1.4 4,261,358 - -0.7 4,261,382 - qor b4051 0.11 +0.1 4,261,382 - qor b4051 0.11 -0.1 4,261,394 - qor b4051 0.12 +1.7 4,261,406 - qor b4051 0.14 +0.2 4,261,406 - qor b4051 0.14 +0.3 4,261,478 - qor b4051 0.21 +0.9 4,261,587 + qor b4051 0.32 -0.0 4,261,593 - qor b4051 0.33 -0.2 4,261,610 + qor b4051 0.34 +1.5 4,261,698 - qor b4051 0.43 -0.0 4,261,698 - qor b4051 0.43 -0.1 4,261,955 + qor b4051 0.70 +0.8 4,261,955 - qor b4051 0.70 -0.0 4,261,977 + qor b4051 0.72 -0.3 4,261,984 - qor b4051 0.72 +0.0 4,261,991 + qor b4051 0.73 -0.4 4,262,007 - qor b4051 0.75 -1.1 4,262,007 - qor b4051 0.75 +0.5 4,262,007 - qor b4051 0.75 -0.9 4,262,042 - qor b4051 0.78 +1.4 4,262,079 - qor b4051 0.82 +0.0 4,262,195 - +0.4 4,263,790 + -0.4 4,263,828 - +0.5 4,263,828 - +0.4 4,263,871 - +0.7 4,263,919 + alr b4053 0.11 +0.8 4,263,928 - alr b4053 0.11 -1.3 4,264,017 + alr b4053 0.20 +0.3 4,264,017 + alr b4053 0.20 -1.1 4,264,102 - alr b4053 0.28 +0.2 4,264,142 - alr b4053 0.31 +0.2 4,264,146 - alr b4053 0.32 +0.3 4,264,146 - alr b4053 0.32 -1.1 4,264,220 - alr b4053 0.38 +0.3 4,264,232 + alr b4053 0.40 -0.2 4,264,240 - alr b4053 0.40 +0.1 4,264,240 - alr b4053 0.40 +0.6 4,264,240 - alr b4053 0.40 +0.4 4,264,240 - alr b4053 0.40 +0.1 4,264,266 + alr b4053 0.43 +0.2 4,264,406 - alr b4053 0.56 +0.2 4,264,406 - alr b4053 0.56 +0.8 4,264,424 + alr b4053 0.57 +0.2 4,264,462 + alr b4053 0.61 -0.2 4,264,525 - alr b4053 0.67 -0.3 4,264,525 - alr b4053 0.67 +0.9 4,264,525 - alr b4053 0.67 -0.1 4,264,525 - alr b4053 0.67 -0.0 4,264,620 + alr b4053 0.75 +1.4 4,264,620 + alr b4053 0.75 +0.2 4,264,639 - alr b4053 0.77 +0.5 4,264,658 + alr b4053 0.79 +0.5 4,264,658 + alr b4053 0.79 +0.0 4,264,661 + alr b4053 0.79 +0.4 4,264,661 + alr b4053 0.79 +0.8 4,264,661 - alr b4053 0.79 -0.1 4,264,664 - alr b4053 0.80 +0.0 4,264,722 - alr b4053 0.85 -0.3 4,264,729 + alr b4053 0.86 +0.1
Or see this region's nucleotide sequence