Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaN and dnaA are separated by 4 nucleotides dnaA and rpmH are separated by 606 nucleotides rpmH and rnpA are separated by 16 nucleotides
b3701: dnaN - DNA polymerase III subunit beta (NCBI), at 3,879,244 to 3,880,344
dnaN
b3702: dnaA - chromosomal replication initiation protein (NCBI), at 3,880,349 to 3,881,752
dnaA
b3703: rpmH - 50S ribosomal protein L34 (NCBI), at 3,882,359 to 3,882,499
rpmH
b3704: rnpA - ribonuclease P (NCBI), at 3,882,516 to 3,882,875
rnpA
Position (kb)
3880
3881
3882 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3881.798 kb on - strand at 3881.847 kb on - strand at 3881.847 kb on - strand at 3881.884 kb on - strand at 3881.914 kb on + strand at 3881.932 kb on - strand at 3881.959 kb on + strand at 3881.972 kb on - strand at 3881.976 kb on + strand at 3882.000 kb on - strand at 3882.000 kb on - strand at 3882.009 kb on - strand at 3882.088 kb on + strand at 3882.097 kb on - strand at 3882.110 kb on + strand at 3882.110 kb on + strand at 3882.118 kb on - strand at 3882.118 kb on - strand at 3882.120 kb on - strand at 3882.163 kb on + strand at 3882.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 3,881,798 - +0.6 3,881,847 - -0.7 3,881,847 - -0.3 3,881,884 - -0.5 3,881,914 + +1.1 3,881,932 - -1.8 3,881,959 + -0.5 3,881,972 - -2.8 3,881,976 + -0.1 3,882,000 - -1.3 3,882,000 - -0.9 3,882,009 - -2.4 3,882,088 + +1.6 3,882,097 - -0.6 3,882,110 + -0.4 3,882,110 + -0.9 3,882,118 - -0.5 3,882,118 - -1.2 3,882,120 - +0.3 3,882,163 + -1.6 3,882,172 - +0.0
Or see this region's nucleotide sequence