Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recF and dnaN overlap by 1 nucleotides dnaN and dnaA are separated by 4 nucleotides
b3700: recF - recombination protein F (NCBI), at 3,878,171 to 3,879,244
recF
b3701: dnaN - DNA polymerase III subunit beta (NCBI), at 3,879,244 to 3,880,344
dnaN
b3702: dnaA - chromosomal replication initiation protein (NCBI), at 3,880,349 to 3,881,752
dnaA
Position (kb)
3879
3880
3881 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3878.247 kb on - strand at 3878.247 kb on - strand at 3878.257 kb on - strand at 3878.322 kb on - strand, within recF at 3878.376 kb on - strand, within recF at 3878.407 kb on - strand, within recF at 3878.407 kb on - strand, within recF at 3878.489 kb on + strand, within recF at 3878.503 kb on - strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.513 kb on - strand, within recF at 3878.559 kb on - strand at 3878.559 kb on - strand at 3878.565 kb on - strand, within recF at 3878.628 kb on + strand, within recF at 3878.655 kb on + strand, within recF at 3878.655 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.660 kb on + strand, within recF at 3878.665 kb on - strand, within recF at 3878.665 kb on - strand, within recF at 3878.699 kb on + strand, within recF at 3878.707 kb on + strand, within recF at 3878.714 kb on - strand, within recF at 3878.736 kb on - strand, within recF at 3878.736 kb on - strand, within recF at 3878.742 kb on + strand, within recF at 3878.757 kb on + strand, within recF at 3878.757 kb on + strand, within recF at 3878.760 kb on - strand, within recF at 3878.789 kb on + strand, within recF at 3878.837 kb on + strand, within recF at 3878.837 kb on + strand, within recF at 3878.878 kb on - strand, within recF at 3878.883 kb on - strand, within recF at 3878.912 kb on + strand, within recF at 3878.912 kb on + strand, within recF at 3878.915 kb on - strand, within recF at 3878.915 kb on - strand, within recF at 3878.926 kb on - strand, within recF at 3878.926 kb on - strand, within recF at 3878.941 kb on + strand, within recF at 3878.949 kb on - strand, within recF at 3878.954 kb on - strand, within recF at 3878.982 kb on + strand, within recF at 3878.991 kb on - strand, within recF at 3878.992 kb on + strand, within recF at 3879.003 kb on + strand, within recF at 3879.052 kb on + strand, within recF at 3879.052 kb on + strand, within recF at 3879.098 kb on + strand, within recF at 3879.099 kb on - strand, within recF at 3879.117 kb on - strand, within recF at 3879.119 kb on - strand, within recF at 3879.120 kb on - strand, within recF at 3879.120 kb on - strand, within recF at 3879.181 kb on - strand at 3879.185 kb on + strand at 3879.220 kb on - strand at 3879.242 kb on - strand at 3879.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 3,878,247 - +0.1 3,878,247 - -0.7 3,878,257 - -1.1 3,878,322 - recF b3700 0.14 -0.0 3,878,376 - recF b3700 0.19 -1.0 3,878,407 - recF b3700 0.22 -1.1 3,878,407 - recF b3700 0.22 -0.8 3,878,489 + recF b3700 0.30 -0.1 3,878,503 - recF b3700 0.31 -0.3 3,878,505 + recF b3700 0.31 -0.8 3,878,505 + recF b3700 0.31 -0.4 3,878,505 + recF b3700 0.31 -0.5 3,878,505 + recF b3700 0.31 +0.4 3,878,505 + recF b3700 0.31 +0.1 3,878,505 + recF b3700 0.31 -0.4 3,878,513 - recF b3700 0.32 -0.2 3,878,559 - +0.1 3,878,559 - +0.7 3,878,565 - recF b3700 0.37 -1.3 3,878,628 + recF b3700 0.43 -0.2 3,878,655 + recF b3700 0.45 +0.1 3,878,655 + recF b3700 0.45 +0.3 3,878,657 + recF b3700 0.45 +0.0 3,878,657 + recF b3700 0.45 -0.2 3,878,657 + recF b3700 0.45 -1.1 3,878,657 + recF b3700 0.45 -0.1 3,878,660 + recF b3700 0.46 -0.4 3,878,665 - recF b3700 0.46 -1.6 3,878,665 - recF b3700 0.46 -1.5 3,878,699 + recF b3700 0.49 -1.6 3,878,707 + recF b3700 0.50 +0.3 3,878,714 - recF b3700 0.51 -0.5 3,878,736 - recF b3700 0.53 -0.1 3,878,736 - recF b3700 0.53 -1.5 3,878,742 + recF b3700 0.53 -0.2 3,878,757 + recF b3700 0.55 -0.5 3,878,757 + recF b3700 0.55 -0.3 3,878,760 - recF b3700 0.55 -0.8 3,878,789 + recF b3700 0.58 -0.1 3,878,837 + recF b3700 0.62 -0.0 3,878,837 + recF b3700 0.62 -1.0 3,878,878 - recF b3700 0.66 -0.7 3,878,883 - recF b3700 0.66 +0.0 3,878,912 + recF b3700 0.69 +0.8 3,878,912 + recF b3700 0.69 +0.4 3,878,915 - recF b3700 0.69 +0.6 3,878,915 - recF b3700 0.69 +0.1 3,878,926 - recF b3700 0.70 -3.1 3,878,926 - recF b3700 0.70 -0.7 3,878,941 + recF b3700 0.72 -1.0 3,878,949 - recF b3700 0.72 +1.0 3,878,954 - recF b3700 0.73 -0.3 3,878,982 + recF b3700 0.76 -0.2 3,878,991 - recF b3700 0.76 -0.1 3,878,992 + recF b3700 0.76 -0.3 3,879,003 + recF b3700 0.77 -0.9 3,879,052 + recF b3700 0.82 -0.2 3,879,052 + recF b3700 0.82 +0.1 3,879,098 + recF b3700 0.86 -0.5 3,879,099 - recF b3700 0.86 +0.4 3,879,117 - recF b3700 0.88 -0.4 3,879,119 - recF b3700 0.88 -0.1 3,879,120 - recF b3700 0.88 -1.2 3,879,120 - recF b3700 0.88 -0.5 3,879,181 - -0.5 3,879,185 + +0.1 3,879,220 - -0.1 3,879,242 - +0.0 3,879,242 - +1.2
Or see this region's nucleotide sequence