Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and fldB are separated by 111 nucleotides fldB and ygfX are separated by 39 nucleotides ygfX and ygfY overlap by 20 nucleotides ygfY and ygfZ are separated by 242 nucleotides
b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765
xerD
b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398
fldB
b2896: ygfX - hypothetical protein (NCBI), at 3,038,438 to 3,038,845
ygfX
b2897: ygfY - hypothetical protein (NCBI), at 3,038,826 to 3,039,092
ygfY
b2898: ygfZ - putative global regulator (NCBI), at 3,039,335 to 3,040,315
ygfZ
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3037.372 kb on + strand, within xerD at 3037.773 kb on + strand at 3037.777 kb on + strand at 3037.777 kb on + strand at 3037.900 kb on + strand at 3037.900 kb on + strand at 3037.986 kb on - strand, within fldB at 3038.097 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.321 kb on + strand, within fldB at 3038.348 kb on - strand at 3038.348 kb on - strand at 3038.418 kb on - strand at 3038.516 kb on - strand, within ygfX at 3038.698 kb on - strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.716 kb on - strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.746 kb on - strand, within ygfX at 3038.776 kb on + strand, within ygfX at 3038.785 kb on - strand, within ygfX at 3038.785 kb on - strand, within ygfX at 3038.869 kb on + strand, within ygfY at 3038.877 kb on - strand, within ygfY at 3038.877 kb on - strand, within ygfY at 3038.992 kb on - strand, within ygfY at 3039.002 kb on - strand, within ygfY at 3039.028 kb on + strand, within ygfY at 3039.170 kb on - strand at 3039.170 kb on - strand at 3039.251 kb on - strand at 3039.251 kb on - strand at 3039.255 kb on - strand at 3039.298 kb on + strand at 3039.320 kb on + strand at 3039.320 kb on + strand at 3039.328 kb on - strand at 3039.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 3,037,372 + xerD b2894 0.56 -0.4 3,037,773 + -0.8 3,037,777 + -0.0 3,037,777 + -0.3 3,037,900 + -1.5 3,037,900 + -0.8 3,037,986 - fldB b2895 0.21 -1.0 3,038,097 + fldB b2895 0.42 -0.7 3,038,128 + fldB b2895 0.48 -0.3 3,038,128 + fldB b2895 0.48 -0.7 3,038,128 + fldB b2895 0.48 -1.1 3,038,149 + fldB b2895 0.52 -0.8 3,038,149 + fldB b2895 0.52 -1.9 3,038,321 + fldB b2895 0.85 -1.3 3,038,348 - -0.6 3,038,348 - -1.1 3,038,418 - +0.1 3,038,516 - ygfX b2896 0.19 -0.5 3,038,698 - ygfX b2896 0.64 -0.3 3,038,714 + ygfX b2896 0.68 -0.7 3,038,714 + ygfX b2896 0.68 +0.9 3,038,716 - ygfX b2896 0.68 +0.2 3,038,718 + ygfX b2896 0.69 -0.3 3,038,718 + ygfX b2896 0.69 -0.6 3,038,746 - ygfX b2896 0.75 +0.2 3,038,776 + ygfX b2896 0.83 -0.6 3,038,785 - ygfX b2896 0.85 -0.5 3,038,785 - ygfX b2896 0.85 +0.8 3,038,869 + ygfY b2897 0.16 +0.5 3,038,877 - ygfY b2897 0.19 +0.7 3,038,877 - ygfY b2897 0.19 +2.6 3,038,992 - ygfY b2897 0.62 -1.1 3,039,002 - ygfY b2897 0.66 -0.2 3,039,028 + ygfY b2897 0.76 -0.1 3,039,170 - +0.4 3,039,170 - -1.6 3,039,251 - -0.5 3,039,251 - +0.3 3,039,255 - +0.1 3,039,298 + +0.5 3,039,320 + -0.7 3,039,320 + -0.4 3,039,328 - -0.4 3,039,379 - -1.9
Or see this region's nucleotide sequence