Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfgC and yfgD are separated by 20 nucleotides yfgD and hda are separated by 137 nucleotides hda and uraA are separated by 94 nucleotides
b2494: yfgC - predicted peptidase (NCBI), at 2,614,116 to 2,615,579
yfgC
b2495: yfgD - predicted oxidoreductase (NCBI), at 2,615,600 to 2,615,959
yfgD
b2496: hda - ATPase regulatory factor involved in DnaA inactivation (RefSeq), at 2,616,097 to 2,616,798
hda
b2497: uraA - uracil transporter (NCBI), at 2,616,893 to 2,618,182
uraA
Position (kb)
2616
2617 Strain fitness (log2 ratio)
-1
0
1 at 2615.356 kb on - strand, within yfgC at 2615.392 kb on - strand, within yfgC at 2615.403 kb on - strand, within yfgC at 2615.463 kb on - strand at 2615.463 kb on - strand at 2615.470 kb on + strand at 2615.470 kb on - strand at 2615.470 kb on - strand at 2615.719 kb on - strand, within yfgD at 2615.756 kb on + strand, within yfgD at 2615.766 kb on - strand, within yfgD at 2615.766 kb on - strand, within yfgD at 2615.826 kb on - strand, within yfgD at 2615.826 kb on - strand, within yfgD at 2615.826 kb on - strand, within yfgD at 2615.826 kb on - strand, within yfgD at 2615.857 kb on - strand, within yfgD at 2615.857 kb on - strand, within yfgD at 2615.898 kb on - strand, within yfgD at 2615.953 kb on - strand at 2615.966 kb on - strand at 2616.025 kb on + strand at 2616.867 kb on + strand at 2616.867 kb on + strand at 2616.869 kb on + strand at 2616.955 kb on - strand at 2616.955 kb on - strand at 2617.019 kb on - strand at 2617.096 kb on - strand, within uraA at 2617.336 kb on - strand, within uraA at 2617.346 kb on - strand, within uraA at 2617.391 kb on + strand, within uraA at 2617.399 kb on - strand, within uraA at 2617.399 kb on - strand, within uraA at 2617.404 kb on - strand, within uraA at 2617.436 kb on - strand, within uraA at 2617.492 kb on - strand, within uraA at 2617.572 kb on + strand, within uraA at 2617.675 kb on - strand, within uraA at 2617.675 kb on - strand, within uraA at 2617.690 kb on + strand, within uraA at 2617.695 kb on + strand, within uraA
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 2,615,356 - yfgC b2494 0.85 -0.1 2,615,392 - yfgC b2494 0.87 -0.7 2,615,403 - yfgC b2494 0.88 -0.2 2,615,463 - +0.2 2,615,463 - +0.3 2,615,470 + -0.9 2,615,470 - -0.1 2,615,470 - -0.2 2,615,719 - yfgD b2495 0.33 -0.1 2,615,756 + yfgD b2495 0.43 +0.0 2,615,766 - yfgD b2495 0.46 +0.1 2,615,766 - yfgD b2495 0.46 -0.3 2,615,826 - yfgD b2495 0.63 -0.4 2,615,826 - yfgD b2495 0.63 +0.4 2,615,826 - yfgD b2495 0.63 +0.9 2,615,826 - yfgD b2495 0.63 -1.6 2,615,857 - yfgD b2495 0.71 +0.8 2,615,857 - yfgD b2495 0.71 -0.4 2,615,898 - yfgD b2495 0.83 -1.7 2,615,953 - -0.2 2,615,966 - -1.5 2,616,025 + -0.3 2,616,867 + -0.6 2,616,867 + -0.4 2,616,869 + -0.5 2,616,955 - -0.3 2,616,955 - -0.3 2,617,019 - +0.2 2,617,096 - uraA b2497 0.16 -0.0 2,617,336 - uraA b2497 0.34 +0.5 2,617,346 - uraA b2497 0.35 -0.5 2,617,391 + uraA b2497 0.39 +0.3 2,617,399 - uraA b2497 0.39 +0.5 2,617,399 - uraA b2497 0.39 +0.7 2,617,404 - uraA b2497 0.40 +0.2 2,617,436 - uraA b2497 0.42 +1.0 2,617,492 - uraA b2497 0.46 +0.3 2,617,572 + uraA b2497 0.53 -0.5 2,617,675 - uraA b2497 0.61 +0.0 2,617,675 - uraA b2497 0.61 +0.1 2,617,690 + uraA b2497 0.62 +0.3 2,617,695 + uraA b2497 0.62 +0.2
Or see this region's nucleotide sequence