Strain Fitness in Escherichia coli BW25113 around b2435

Experiment: P2 dilution 10-3 cacl2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	P2 dilution 10-3 cacl2
2,549,388	+	b2433	b2433	0.20	+0.2
2,549,645	+	b2433	b2433	0.77	-1.0
2,549,680	+	b2433	b2433	0.85	+0.3
2,549,680	+	b2433	b2433	0.85	-1.5
2,549,746	-				-0.1
2,549,795	+	b2434	b2434	0.14	+3.0
2,549,833	-	b2434	b2434	0.23	+0.4
2,549,864	+	b2434	b2434	0.30	-0.1
2,550,001	+	b2434	b2434	0.62	-0.9
2,550,037	+	b2434	b2434	0.71	+0.3
2,550,357	+				+0.9
2,550,357	+				-0.2
2,550,381	+				+0.5
2,550,597	-	amiA	b2435	0.26	-0.7
2,550,631	-	amiA	b2435	0.30	-0.4
2,550,747	+	amiA	b2435	0.43	-0.5
2,550,779	+	amiA	b2435	0.47	-0.3
2,550,779	+	amiA	b2435	0.47	-0.8
2,551,032	+	amiA	b2435	0.76	-0.4
2,551,066	-	amiA	b2435	0.80	-0.9
2,551,197	+				-0.3
2,551,266	+				+0.1
2,551,295	-				+1.3
2,551,295	-				-0.1
2,551,355	-	hemF	b2436	0.12	-0.5
2,551,444	+	hemF	b2436	0.22	+0.9
2,551,444	+	hemF	b2436	0.22	-1.0
2,551,531	-	hemF	b2436	0.32	-0.2
2,551,643	-	hemF	b2436	0.44	-0.3
2,551,645	+	hemF	b2436	0.44	+0.1
2,551,678	+	hemF	b2436	0.48	+0.0
2,551,678	+	hemF	b2436	0.48	+0.3
2,551,837	+	hemF	b2436	0.66	-0.3
2,551,837	+	hemF	b2436	0.66	+0.6
2,552,065	-				-0.1
2,552,065	-				-0.5
2,552,087	+				+0.2
2,552,087	+				-0.3
2,552,141	+				-0.2