Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfS and mfd are separated by 143 nucleotides mfd and ycfT are separated by 127 nucleotides
b1113: ycfS - hypothetical protein (NCBI), at 1,168,635 to 1,169,597
ycfS
b1114: mfd - transcription-repair coupling factor (NCBI), at 1,169,741 to 1,173,187
mfd
b1115: ycfT - predicted inner membrane protein (NCBI), at 1,173,315 to 1,174,388
ycfT
Position (kb)
1169
1170
1171
1172
1173
1174 Strain fitness (log2 ratio)
-1
0
1 at 1168.746 kb on + strand, within ycfS at 1168.766 kb on + strand, within ycfS at 1168.802 kb on + strand, within ycfS at 1168.805 kb on + strand, within ycfS at 1168.870 kb on + strand, within ycfS at 1168.925 kb on - strand, within ycfS at 1169.013 kb on + strand, within ycfS at 1169.180 kb on + strand, within ycfS at 1169.204 kb on + strand, within ycfS at 1169.204 kb on + strand, within ycfS at 1169.259 kb on - strand, within ycfS at 1169.270 kb on + strand, within ycfS at 1169.654 kb on - strand at 1169.664 kb on + strand at 1169.664 kb on + strand at 1169.666 kb on + strand at 1169.674 kb on + strand at 1169.674 kb on + strand at 1169.682 kb on - strand at 1170.000 kb on + strand at 1170.000 kb on + strand at 1170.148 kb on - strand, within mfd at 1170.304 kb on - strand, within mfd at 1170.667 kb on + strand, within mfd at 1170.747 kb on + strand, within mfd at 1170.747 kb on + strand, within mfd at 1170.806 kb on + strand, within mfd at 1170.880 kb on - strand, within mfd at 1170.970 kb on + strand, within mfd at 1170.970 kb on + strand, within mfd at 1171.193 kb on + strand, within mfd at 1171.218 kb on + strand, within mfd at 1171.228 kb on - strand, within mfd at 1171.228 kb on - strand, within mfd at 1171.252 kb on - strand, within mfd at 1171.252 kb on - strand, within mfd at 1171.294 kb on + strand, within mfd at 1171.294 kb on + strand, within mfd at 1171.403 kb on + strand, within mfd at 1171.451 kb on + strand, within mfd at 1171.655 kb on + strand, within mfd at 1171.939 kb on + strand, within mfd at 1172.040 kb on - strand, within mfd at 1172.040 kb on - strand, within mfd at 1172.210 kb on - strand, within mfd at 1172.574 kb on - strand, within mfd at 1172.758 kb on - strand, within mfd at 1172.772 kb on + strand, within mfd at 1172.872 kb on + strand at 1172.925 kb on + strand at 1172.941 kb on + strand at 1173.058 kb on + strand at 1173.058 kb on + strand at 1173.058 kb on + strand at 1173.058 kb on + strand at 1173.071 kb on + strand at 1173.089 kb on + strand at 1173.148 kb on - strand at 1173.173 kb on - strand at 1173.192 kb on + strand at 1173.218 kb on - strand at 1173.463 kb on - strand, within ycfT at 1173.506 kb on - strand, within ycfT at 1173.506 kb on - strand, within ycfT at 1173.541 kb on - strand, within ycfT at 1173.593 kb on + strand, within ycfT at 1173.602 kb on - strand, within ycfT at 1173.602 kb on - strand, within ycfT at 1173.710 kb on + strand, within ycfT at 1173.720 kb on + strand, within ycfT at 1173.720 kb on + strand, within ycfT at 1173.747 kb on + strand, within ycfT at 1173.757 kb on - strand, within ycfT at 1173.757 kb on - strand, within ycfT at 1173.767 kb on - strand, within ycfT at 1173.791 kb on + strand, within ycfT at 1173.791 kb on + strand, within ycfT at 1174.091 kb on - strand, within ycfT at 1174.091 kb on - strand, within ycfT at 1174.111 kb on + strand, within ycfT at 1174.176 kb on - strand, within ycfT at 1174.176 kb on - strand, within ycfT
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 1,168,746 + ycfS b1113 0.12 -0.1 1,168,766 + ycfS b1113 0.14 -0.2 1,168,802 + ycfS b1113 0.17 -0.2 1,168,805 + ycfS b1113 0.18 +1.3 1,168,870 + ycfS b1113 0.24 +0.1 1,168,925 - ycfS b1113 0.30 -0.4 1,169,013 + ycfS b1113 0.39 -0.7 1,169,180 + ycfS b1113 0.57 +0.7 1,169,204 + ycfS b1113 0.59 -0.7 1,169,204 + ycfS b1113 0.59 +0.9 1,169,259 - ycfS b1113 0.65 +0.4 1,169,270 + ycfS b1113 0.66 +0.7 1,169,654 - -0.2 1,169,664 + +0.1 1,169,664 + -0.1 1,169,666 + -0.7 1,169,674 + -0.2 1,169,674 + -0.4 1,169,682 - -0.4 1,170,000 + +0.9 1,170,000 + -1.6 1,170,148 - mfd b1114 0.12 +0.6 1,170,304 - mfd b1114 0.16 +0.8 1,170,667 + mfd b1114 0.27 +0.5 1,170,747 + mfd b1114 0.29 +0.9 1,170,747 + mfd b1114 0.29 -0.8 1,170,806 + mfd b1114 0.31 -0.3 1,170,880 - mfd b1114 0.33 -1.4 1,170,970 + mfd b1114 0.36 +0.6 1,170,970 + mfd b1114 0.36 -0.2 1,171,193 + mfd b1114 0.42 +0.2 1,171,218 + mfd b1114 0.43 +0.2 1,171,228 - mfd b1114 0.43 +0.3 1,171,228 - mfd b1114 0.43 -1.0 1,171,252 - mfd b1114 0.44 -0.4 1,171,252 - mfd b1114 0.44 -1.2 1,171,294 + mfd b1114 0.45 -0.1 1,171,294 + mfd b1114 0.45 -0.4 1,171,403 + mfd b1114 0.48 -0.4 1,171,451 + mfd b1114 0.50 -0.4 1,171,655 + mfd b1114 0.56 -1.3 1,171,939 + mfd b1114 0.64 +0.1 1,172,040 - mfd b1114 0.67 +1.1 1,172,040 - mfd b1114 0.67 +1.4 1,172,210 - mfd b1114 0.72 +0.7 1,172,574 - mfd b1114 0.82 -0.2 1,172,758 - mfd b1114 0.88 +0.1 1,172,772 + mfd b1114 0.88 -0.3 1,172,872 + +1.0 1,172,925 + -0.8 1,172,941 + -0.2 1,173,058 + -0.6 1,173,058 + -0.1 1,173,058 + -0.5 1,173,058 + -0.8 1,173,071 + -0.2 1,173,089 + +0.5 1,173,148 - +0.6 1,173,173 - -0.1 1,173,192 + -0.3 1,173,218 - -0.6 1,173,463 - ycfT b1115 0.14 +1.7 1,173,506 - ycfT b1115 0.18 -1.4 1,173,506 - ycfT b1115 0.18 +0.2 1,173,541 - ycfT b1115 0.21 -0.2 1,173,593 + ycfT b1115 0.26 +0.2 1,173,602 - ycfT b1115 0.27 -0.7 1,173,602 - ycfT b1115 0.27 -0.5 1,173,710 + ycfT b1115 0.37 +0.5 1,173,720 + ycfT b1115 0.38 -0.4 1,173,720 + ycfT b1115 0.38 +0.0 1,173,747 + ycfT b1115 0.40 +0.5 1,173,757 - ycfT b1115 0.41 +0.2 1,173,757 - ycfT b1115 0.41 -0.1 1,173,767 - ycfT b1115 0.42 +0.3 1,173,791 + ycfT b1115 0.44 -0.8 1,173,791 + ycfT b1115 0.44 +0.4 1,174,091 - ycfT b1115 0.72 -0.7 1,174,091 - ycfT b1115 0.72 -0.0 1,174,111 + ycfT b1115 0.74 +0.6 1,174,176 - ycfT b1115 0.80 +0.1 1,174,176 - ycfT b1115 0.80 +0.1
Or see this region's nucleotide sequence