Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobC and nadD are separated by 23 nucleotides nadD and holA are separated by 1 nucleotides holA and rlpB overlap by 1 nucleotides rlpB and leuS are separated by 14 nucleotides
b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130
cobC
b0639: nadD - nicotinic acid mononucleotide adenyltransferase (NCBI), at 669,154 to 669,795
nadD
b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828
holA
b0641: rlpB - minor lipoprotein (NCBI), at 670,828 to 671,409
rlpB
b0642: leuS - leucyl-tRNA synthetase (NCBI), at 671,424 to 674,006
leuS
Position (kb) 669
670
671 Strain fitness (log2 ratio) -2
-1
0
1 at 668.930 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.992 kb on + strand, within cobC at 669.031 kb on + strand, within cobC at 669.048 kb on + strand, within cobC at 669.057 kb on - strand, within cobC at 669.057 kb on - strand, within cobC at 669.095 kb on - strand at 669.153 kb on - strand at 669.158 kb on - strand at 669.158 kb on - strand at 669.179 kb on + strand at 669.187 kb on - strand at 669.852 kb on + strand at 670.785 kb on + strand at 670.837 kb on - strand at 670.880 kb on - strand at 670.915 kb on - strand, within rlpB at 670.922 kb on - strand, within rlpB at 671.394 kb on - strand at 671.404 kb on - strand at 671.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 668,930 + cobC b0638 0.67 +0.2 668,948 + cobC b0638 0.70 -1.9 668,948 + cobC b0638 0.70 +0.1 668,992 + cobC b0638 0.77 +0.3 669,031 + cobC b0638 0.84 -0.1 669,048 + cobC b0638 0.86 -0.4 669,057 - cobC b0638 0.88 -0.2 669,057 - cobC b0638 0.88 +0.6 669,095 - -0.5 669,153 - -0.9 669,158 - +0.5 669,158 - -0.7 669,179 + -1.6 669,187 - +0.1 669,852 + -0.5 670,785 + -0.8 670,837 - -0.6 670,880 - -0.4 670,915 - rlpB b0641 0.15 -0.4 670,922 - rlpB b0641 0.16 +0.6 671,394 - +0.1 671,404 - +0.1 671,404 - -0.6
Or see this region's nucleotide sequence