Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaN and apt are separated by 152 nucleotides apt and dnaX are separated by 128 nucleotides dnaX and ybaB are separated by 52 nucleotides ybaB and recR overlap by 1 nucleotides
b0468: ybaN - conserved inner membrane protein (NCBI), at 490,106 to 490,483
ybaN
b0469: apt - adenine phosphoribosyltransferase (NCBI), at 490,636 to 491,187
apt
b0470: dnaX - DNA polymerase III subunits gamma and tau (NCBI), at 491,316 to 493,247
dnaX
b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629
ybaB
b0472: recR - recombination protein RecR (NCBI), at 493,629 to 494,234
recR
Position (kb) 491
492
493
494 Strain fitness (log2 ratio) -2
-1
0
1 at 490.328 kb on - strand, within ybaN at 490.367 kb on + strand, within ybaN at 490.379 kb on + strand, within ybaN at 490.392 kb on + strand, within ybaN at 490.432 kb on + strand, within ybaN at 490.445 kb on - strand, within ybaN at 490.445 kb on - strand, within ybaN at 490.485 kb on - strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.625 kb on + strand at 490.695 kb on - strand, within apt at 490.731 kb on - strand, within apt at 490.731 kb on - strand, within apt at 490.734 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.828 kb on - strand, within apt at 490.836 kb on - strand, within apt at 490.836 kb on - strand, within apt at 490.866 kb on - strand, within apt at 490.866 kb on - strand, within apt at 490.983 kb on - strand, within apt at 491.004 kb on + strand, within apt at 491.004 kb on + strand, within apt at 491.017 kb on + strand, within apt at 491.017 kb on + strand, within apt at 491.024 kb on - strand, within apt at 491.038 kb on + strand, within apt at 491.046 kb on - strand, within apt at 491.064 kb on + strand, within apt at 491.082 kb on + strand, within apt at 491.197 kb on + strand at 491.212 kb on + strand at 491.221 kb on - strand at 491.221 kb on - strand at 491.223 kb on + strand at 491.224 kb on + strand at 491.224 kb on + strand at 491.224 kb on - strand at 491.308 kb on - strand at 491.308 kb on - strand at 493.296 kb on + strand at 493.296 kb on + strand at 493.320 kb on + strand at 493.327 kb on - strand at 493.392 kb on + strand, within ybaB at 493.488 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.517 kb on + strand, within ybaB at 493.713 kb on - strand, within recR at 493.724 kb on + strand, within recR at 493.879 kb on + strand, within recR at 493.882 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.949 kb on + strand, within recR at 493.993 kb on + strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.018 kb on + strand, within recR at 494.057 kb on + strand, within recR at 494.074 kb on + strand, within recR at 494.094 kb on - strand, within recR at 494.099 kb on - strand, within recR at 494.115 kb on + strand, within recR at 494.211 kb on - strand at 494.211 kb on - strand at 494.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 490,328 - ybaN b0468 0.59 -0.1 490,367 + ybaN b0468 0.69 +1.0 490,379 + ybaN b0468 0.72 -0.4 490,392 + ybaN b0468 0.76 +0.7 490,432 + ybaN b0468 0.86 +0.2 490,445 - ybaN b0468 0.90 +0.1 490,445 - ybaN b0468 0.90 +0.4 490,485 - -0.5 490,625 + -0.0 490,625 + +0.1 490,625 + +0.1 490,625 + +0.3 490,695 - apt b0469 0.11 -1.0 490,731 - apt b0469 0.17 -1.0 490,731 - apt b0469 0.17 +0.3 490,734 - apt b0469 0.18 -0.8 490,828 - apt b0469 0.35 -0.8 490,828 - apt b0469 0.35 +0.4 490,828 - apt b0469 0.35 -0.3 490,828 - apt b0469 0.35 -2.7 490,836 - apt b0469 0.36 -2.3 490,836 - apt b0469 0.36 -0.9 490,866 - apt b0469 0.42 -1.3 490,866 - apt b0469 0.42 -0.9 490,983 - apt b0469 0.63 -0.6 491,004 + apt b0469 0.67 +0.3 491,004 + apt b0469 0.67 -1.1 491,017 + apt b0469 0.69 +0.4 491,017 + apt b0469 0.69 -0.6 491,024 - apt b0469 0.70 -0.3 491,038 + apt b0469 0.73 -0.7 491,046 - apt b0469 0.74 -1.0 491,064 + apt b0469 0.78 +0.5 491,082 + apt b0469 0.81 -0.2 491,197 + -1.1 491,212 + -0.5 491,221 - +0.2 491,221 - -0.3 491,223 + +0.4 491,224 + -0.4 491,224 + +0.5 491,224 - +0.1 491,308 - +0.2 491,308 - -0.5 493,296 + +0.8 493,296 + -0.2 493,320 + -0.2 493,327 - +0.4 493,392 + ybaB b0471 0.28 -0.3 493,488 - ybaB b0471 0.57 +0.2 493,499 - ybaB b0471 0.60 -1.8 493,499 - ybaB b0471 0.60 -0.7 493,517 + ybaB b0471 0.66 +0.1 493,713 - recR b0472 0.14 -0.6 493,724 + recR b0472 0.16 -0.8 493,879 + recR b0472 0.41 -0.9 493,882 - recR b0472 0.42 -0.1 493,887 - recR b0472 0.43 -0.3 493,887 - recR b0472 0.43 -1.1 493,949 + recR b0472 0.53 -2.0 493,993 + recR b0472 0.60 +0.1 494,016 - recR b0472 0.64 -0.6 494,016 - recR b0472 0.64 +1.4 494,016 - recR b0472 0.64 -1.5 494,018 + recR b0472 0.64 -0.2 494,057 + recR b0472 0.71 -1.0 494,074 + recR b0472 0.73 -0.0 494,094 - recR b0472 0.77 -0.0 494,099 - recR b0472 0.78 -0.1 494,115 + recR b0472 0.80 -0.8 494,211 - -0.5 494,211 - -0.5 494,211 - -0.2
Or see this region's nucleotide sequence