Strain Fitness in Escherichia coli BW25113 around b0143

Experiment: P2 dilution 10-3 cacl2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyadN and folK are separated by 369 nucleotidesfolK and pcnB overlap by 4 nucleotidespcnB and yadB are separated by 59 nucleotides b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883 yadN b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732 folK b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126 pcnB b0144: yadB - glutamyl-Q tRNA(Asp) synthetase (RefSeq), at 159,186 to 160,112 yadB Position (kb) 157 158 159 160Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 156.734 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.744 kb on + strand, within yadNat 156.755 kb on + strand, within yadNat 156.757 kb on + strand, within yadNat 156.759 kb on + strand, within yadNat 156.786 kb on + strand, within yadNat 156.789 kb on + strand, within yadNat 156.793 kb on + strand, within yadNat 156.804 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.825 kb on + strandat 156.827 kb on + strandat 156.827 kb on + strandat 156.834 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.850 kb on + strandat 156.854 kb on + strandat 156.854 kb on + strandat 157.218 kb on - strandat 157.279 kb on - strandat 157.291 kb on - strandat 157.301 kb on - strand, within folKat 157.745 kb on + strandat 157.870 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.923 kb on - strand, within pcnBat 158.143 kb on + strand, within pcnBat 158.156 kb on + strand, within pcnBat 158.227 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.312 kb on - strand, within pcnBat 158.342 kb on - strand, within pcnBat 158.378 kb on - strand, within pcnBat 158.400 kb on + strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.426 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.447 kb on - strand, within pcnBat 158.516 kb on + strand, within pcnBat 158.522 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.864 kb on - strand, within pcnBat 159.038 kb on - strandat 159.093 kb on - strandat 159.093 kb on - strandat 159.133 kb on + strandat 159.347 kb on - strand, within yadBat 159.347 kb on - strand, within yadBat 159.352 kb on - strand, within yadBat 159.352 kb on - strand, within yadBat 159.581 kb on - strand, within yadBat 159.655 kb on + strand, within yadBat 159.699 kb on + strand, within yadBat 159.722 kb on - strand, within yadBat 159.722 kb on - strand, within yadBat 159.743 kb on - strand, within yadBat 159.754 kb on - strand, within yadBat 159.754 kb on - strand, within yadBat 159.770 kb on + strand, within yadBat 159.770 kb on + strand, within yadBat 159.803 kb on + strand, within yadBat 159.808 kb on + strand, within yadBat 159.905 kb on - strand, within yadBat 159.947 kb on + strand, within yadBat 160.046 kb on + strandat 160.068 kb on + strandat 160.068 kb on - strandat 160.068 kb on - strandat 160.070 kb on - strandat 160.078 kb on + strandat 160.078 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2
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156,734 + yadN b0141 0.74 -1.3
156,735 + yadN b0141 0.75 +0.9
156,735 + yadN b0141 0.75 +1.9
156,744 + yadN b0141 0.76 +0.6
156,755 + yadN b0141 0.78 -2.2
156,757 + yadN b0141 0.78 +1.0
156,759 + yadN b0141 0.79 -0.8
156,786 + yadN b0141 0.83 +1.6
156,789 + yadN b0141 0.84 -0.7
156,793 + yadN b0141 0.84 -0.6
156,804 + yadN b0141 0.86 -0.4
156,823 + yadN b0141 0.90 +0.6
156,823 + yadN b0141 0.90 +0.4
156,825 + +0.9
156,827 + +0.4
156,827 + +0.6
156,834 + +3.2
156,849 + +1.8
156,849 + +0.6
156,849 + -0.7
156,850 + -0.7
156,854 + -0.9
156,854 + -1.4
157,218 - -0.4
157,279 - +0.4
157,291 - -0.4
157,301 - folK b0142 0.10 -1.0
157,745 + -2.2
157,870 - pcnB b0143 0.10 -0.4
157,906 - pcnB b0143 0.13 -2.7
157,906 - pcnB b0143 0.13 +1.2
157,923 - pcnB b0143 0.14 -0.4
158,143 + pcnB b0143 0.30 -1.9
158,156 + pcnB b0143 0.31 -1.3
158,227 - pcnB b0143 0.36 +0.3
158,249 - pcnB b0143 0.37 +0.4
158,249 - pcnB b0143 0.37 -3.3
158,312 - pcnB b0143 0.42 -1.2
158,342 - pcnB b0143 0.44 -1.2
158,378 - pcnB b0143 0.46 -0.9
158,400 + pcnB b0143 0.48 -1.1
158,408 - pcnB b0143 0.49 -1.0
158,408 - pcnB b0143 0.49 +0.8
158,426 - pcnB b0143 0.50 +0.8
158,442 - pcnB b0143 0.51 -1.6
158,442 - pcnB b0143 0.51 -0.1
158,447 - pcnB b0143 0.51 -0.9
158,516 + pcnB b0143 0.56 -3.8
158,522 - pcnB b0143 0.57 -0.1
158,759 - pcnB b0143 0.74 -2.2
158,759 - pcnB b0143 0.74 -0.4
158,759 - pcnB b0143 0.74 -0.1
158,864 - pcnB b0143 0.81 -1.1
159,038 - -0.7
159,093 - +0.0
159,093 - -1.0
159,133 + +1.0
159,347 - yadB b0144 0.17 -0.4
159,347 - yadB b0144 0.17 -0.4
159,352 - yadB b0144 0.18 -0.1
159,352 - yadB b0144 0.18 -0.7
159,581 - yadB b0144 0.43 -0.4
159,655 + yadB b0144 0.51 +0.1
159,699 + yadB b0144 0.55 +0.6
159,722 - yadB b0144 0.58 +0.0
159,722 - yadB b0144 0.58 +0.2
159,743 - yadB b0144 0.60 -0.5
159,754 - yadB b0144 0.61 +0.7
159,754 - yadB b0144 0.61 +1.5
159,770 + yadB b0144 0.63 -1.3
159,770 + yadB b0144 0.63 +0.1
159,803 + yadB b0144 0.67 -0.6
159,808 + yadB b0144 0.67 +0.6
159,905 - yadB b0144 0.78 -0.4
159,947 + yadB b0144 0.82 +0.1
160,046 + -0.1
160,068 + -0.4
160,068 - +0.6
160,068 - +0.2
160,070 - -0.0
160,078 + -0.4
160,078 + +0.2

Or see this region's nucleotide sequence