Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5565 and PP_5566 are separated by 216 nucleotides PP_5566 and PP_5567 are separated by 485 nucleotides PP_5567 and PP_5568 are separated by 155 nucleotides PP_5568 and PP_5569 are separated by 33 nucleotides
PP_5565: PP_5565 - conserved protein of unknown function, at 3,500,053 to 3,500,325
_5565
PP_5566: PP_5566 - protein of unknown function, at 3,500,542 to 3,500,781
_5566
PP_5567: PP_5567 - protein of unknown function, at 3,501,267 to 3,501,458
_5567
PP_5568: PP_5568 - conserved membrane protein of unknown function, at 3,501,614 to 3,502,312
_5568
PP_5569: PP_5569 - protein of unknown function of phage origin, at 3,502,346 to 3,502,549
_5569
Position (kb)
3501
3502 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3500.285 kb on + strand, within PP_5565 at 3500.523 kb on + strand at 3500.593 kb on + strand, within PP_5566 at 3500.594 kb on - strand, within PP_5566 at 3500.594 kb on - strand, within PP_5566 at 3500.631 kb on + strand, within PP_5566 at 3500.631 kb on + strand, within PP_5566 at 3500.631 kb on + strand, within PP_5566 at 3500.632 kb on - strand, within PP_5566 at 3500.632 kb on - strand, within PP_5566 at 3500.654 kb on + strand, within PP_5566 at 3500.726 kb on + strand, within PP_5566 at 3500.742 kb on - strand, within PP_5566 at 3500.808 kb on + strand at 3500.808 kb on + strand at 3500.809 kb on - strand at 3500.897 kb on - strand at 3500.979 kb on + strand at 3500.980 kb on - strand at 3500.980 kb on - strand at 3501.026 kb on + strand at 3501.026 kb on + strand at 3501.027 kb on - strand at 3501.027 kb on - strand at 3501.195 kb on + strand at 3501.405 kb on + strand, within PP_5567 at 3501.510 kb on + strand at 3501.511 kb on - strand at 3501.525 kb on - strand at 3501.770 kb on + strand, within PP_5568 at 3501.804 kb on + strand, within PP_5568 at 3501.805 kb on - strand, within PP_5568 at 3501.931 kb on + strand, within PP_5568 at 3501.957 kb on - strand, within PP_5568 at 3502.005 kb on + strand, within PP_5568 at 3502.006 kb on - strand at 3502.011 kb on + strand, within PP_5568 at 3502.097 kb on + strand, within PP_5568 at 3502.097 kb on + strand, within PP_5568 at 3502.194 kb on + strand, within PP_5568 at 3502.226 kb on + strand, within PP_5568 at 3502.247 kb on + strand at 3502.260 kb on + strand at 3502.320 kb on + strand at 3502.320 kb on + strand at 3502.392 kb on + strand, within PP_5569 at 3502.402 kb on - strand, within PP_5569 at 3502.414 kb on - strand, within PP_5569
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 3,500,285 + PP_5565 0.85 -0.6 3,500,523 + -1.7 3,500,593 + PP_5566 0.21 +0.3 3,500,594 - PP_5566 0.22 -1.2 3,500,594 - PP_5566 0.22 -0.2 3,500,631 + PP_5566 0.37 -0.8 3,500,631 + PP_5566 0.37 +1.8 3,500,631 + PP_5566 0.37 +1.5 3,500,632 - PP_5566 0.38 -0.9 3,500,632 - PP_5566 0.38 +0.5 3,500,654 + PP_5566 0.47 +1.5 3,500,726 + PP_5566 0.77 +0.7 3,500,742 - PP_5566 0.83 +1.1 3,500,808 + -0.1 3,500,808 + +1.0 3,500,809 - -4.0 3,500,897 - +0.7 3,500,979 + +1.0 3,500,980 - +2.2 3,500,980 - +0.6 3,501,026 + +1.2 3,501,026 + +1.4 3,501,027 - -3.7 3,501,027 - -0.6 3,501,195 + +0.3 3,501,405 + PP_5567 0.72 +2.0 3,501,510 + +1.8 3,501,511 - +0.3 3,501,525 - -1.2 3,501,770 + PP_5568 0.22 -0.4 3,501,804 + PP_5568 0.27 -1.0 3,501,805 - PP_5568 0.27 +1.1 3,501,931 + PP_5568 0.45 +0.0 3,501,957 - PP_5568 0.49 -0.2 3,502,005 + PP_5568 0.56 +1.2 3,502,006 - +2.6 3,502,011 + PP_5568 0.57 +1.2 3,502,097 + PP_5568 0.69 -0.8 3,502,097 + PP_5568 0.69 +0.6 3,502,194 + PP_5568 0.83 -3.0 3,502,226 + PP_5568 0.88 -0.7 3,502,247 + +2.6 3,502,260 + +0.1 3,502,320 + +1.3 3,502,320 + +0.4 3,502,392 + PP_5569 0.23 -2.4 3,502,402 - PP_5569 0.27 -1.6 3,502,414 - PP_5569 0.33 -2.9
Or see this region's nucleotide sequence