Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4978 and PP_4979 are separated by 169 nucleotides PP_4979 and PP_4980 are separated by 218 nucleotides
PP_4978: PP_4978 - conserved protein of unknown function, at 5,670,496 to 5,671,581
_4978
PP_4979: PP_4979 - putative Periplasmic binding protein, at 5,671,751 to 5,672,539
_4979
PP_4980: PP_4980 - ATP-dependent RNA helicase, at 5,672,758 to 5,674,638
_4980
Position (kb) 5671
5672
5673 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5670.780 kb on - strand, within PP_4978 at 5670.780 kb on - strand, within PP_4978 at 5670.928 kb on + strand, within PP_4978 at 5670.928 kb on + strand, within PP_4978 at 5671.067 kb on - strand, within PP_4978 at 5671.088 kb on - strand, within PP_4978 at 5671.168 kb on + strand, within PP_4978 at 5671.168 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.315 kb on + strand, within PP_4978 at 5671.316 kb on - strand, within PP_4978 at 5671.316 kb on - strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.375 kb on + strand, within PP_4978 at 5671.376 kb on - strand, within PP_4978 at 5671.376 kb on - strand, within PP_4978 at 5671.384 kb on + strand, within PP_4978 at 5671.462 kb on + strand, within PP_4978 at 5671.515 kb on - strand at 5671.718 kb on - strand at 5671.740 kb on - strand at 5671.759 kb on + strand at 5671.759 kb on + strand at 5671.853 kb on + strand, within PP_4979 at 5671.853 kb on + strand, within PP_4979 at 5671.853 kb on + strand, within PP_4979 at 5671.853 kb on + strand, within PP_4979 at 5671.854 kb on - strand, within PP_4979 at 5671.854 kb on - strand, within PP_4979 at 5671.854 kb on - strand, within PP_4979 at 5671.893 kb on - strand, within PP_4979 at 5671.893 kb on - strand, within PP_4979 at 5672.036 kb on + strand, within PP_4979 at 5672.037 kb on - strand, within PP_4979 at 5672.045 kb on + strand, within PP_4979 at 5672.045 kb on + strand, within PP_4979 at 5672.045 kb on + strand, within PP_4979 at 5672.045 kb on + strand, within PP_4979 at 5672.046 kb on - strand, within PP_4979 at 5672.046 kb on - strand, within PP_4979 at 5672.046 kb on - strand, within PP_4979 at 5672.218 kb on + strand, within PP_4979 at 5672.218 kb on + strand, within PP_4979 at 5672.218 kb on + strand, within PP_4979 at 5672.219 kb on - strand, within PP_4979 at 5672.219 kb on - strand, within PP_4979 at 5672.294 kb on + strand, within PP_4979 at 5672.295 kb on - strand, within PP_4979 at 5672.347 kb on - strand, within PP_4979 at 5672.367 kb on - strand, within PP_4979 at 5672.454 kb on - strand, within PP_4979 at 5672.474 kb on + strand at 5672.476 kb on + strand at 5672.476 kb on + strand at 5672.477 kb on - strand at 5672.546 kb on + strand at 5672.546 kb on + strand at 5672.549 kb on - strand at 5672.549 kb on - strand at 5672.818 kb on + strand at 5672.850 kb on + strand at 5672.851 kb on - strand at 5672.902 kb on - strand at 5672.907 kb on + strand at 5673.061 kb on + strand, within PP_4980 at 5673.061 kb on + strand, within PP_4980 at 5673.062 kb on - strand, within PP_4980 at 5673.440 kb on + strand, within PP_4980 at 5673.440 kb on + strand, within PP_4980 at 5673.442 kb on + strand, within PP_4980 at 5673.442 kb on + strand, within PP_4980 at 5673.443 kb on - strand, within PP_4980 at 5673.443 kb on - strand, within PP_4980
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 5,670,780 - PP_4978 0.26 -2.6 5,670,780 - PP_4978 0.26 +0.5 5,670,928 + PP_4978 0.40 +1.0 5,670,928 + PP_4978 0.40 +4.2 5,671,067 - PP_4978 0.53 -3.3 5,671,088 - PP_4978 0.55 -3.3 5,671,168 + PP_4978 0.62 +0.5 5,671,168 + PP_4978 0.62 -2.7 5,671,315 + PP_4978 0.75 +0.8 5,671,315 + PP_4978 0.75 -0.3 5,671,315 + PP_4978 0.75 +0.4 5,671,316 - PP_4978 0.76 +0.8 5,671,316 - PP_4978 0.76 +0.4 5,671,375 + PP_4978 0.81 -1.1 5,671,375 + PP_4978 0.81 +0.1 5,671,375 + PP_4978 0.81 +0.8 5,671,376 - PP_4978 0.81 +0.8 5,671,376 - PP_4978 0.81 +1.1 5,671,384 + PP_4978 0.82 +0.2 5,671,462 + PP_4978 0.89 -0.8 5,671,515 - +0.6 5,671,718 - +1.5 5,671,740 - -0.2 5,671,759 + -3.9 5,671,759 + +0.4 5,671,853 + PP_4979 0.13 -2.2 5,671,853 + PP_4979 0.13 +0.3 5,671,853 + PP_4979 0.13 -0.5 5,671,853 + PP_4979 0.13 -3.6 5,671,854 - PP_4979 0.13 -1.7 5,671,854 - PP_4979 0.13 -1.1 5,671,854 - PP_4979 0.13 -0.5 5,671,893 - PP_4979 0.18 -0.4 5,671,893 - PP_4979 0.18 -0.5 5,672,036 + PP_4979 0.36 -0.3 5,672,037 - PP_4979 0.36 -0.1 5,672,045 + PP_4979 0.37 -2.7 5,672,045 + PP_4979 0.37 -3.8 5,672,045 + PP_4979 0.37 -2.4 5,672,045 + PP_4979 0.37 -0.9 5,672,046 - PP_4979 0.37 -2.4 5,672,046 - PP_4979 0.37 +0.2 5,672,046 - PP_4979 0.37 -0.5 5,672,218 + PP_4979 0.59 -0.0 5,672,218 + PP_4979 0.59 -1.4 5,672,218 + PP_4979 0.59 -0.4 5,672,219 - PP_4979 0.59 +0.3 5,672,219 - PP_4979 0.59 -0.0 5,672,294 + PP_4979 0.69 -0.7 5,672,295 - PP_4979 0.69 +0.6 5,672,347 - PP_4979 0.76 +1.2 5,672,367 - PP_4979 0.78 +0.8 5,672,454 - PP_4979 0.89 -0.2 5,672,474 + -0.2 5,672,476 + +1.7 5,672,476 + +0.9 5,672,477 - -2.6 5,672,546 + +1.5 5,672,546 + -0.5 5,672,549 - +1.7 5,672,549 - +1.0 5,672,818 + +1.9 5,672,850 + -1.8 5,672,851 - +0.7 5,672,902 - -2.5 5,672,907 + -0.7 5,673,061 + PP_4980 0.16 -0.2 5,673,061 + PP_4980 0.16 -1.0 5,673,062 - PP_4980 0.16 -0.1 5,673,440 + PP_4980 0.36 +2.8 5,673,440 + PP_4980 0.36 -2.0 5,673,442 + PP_4980 0.36 +0.9 5,673,442 + PP_4980 0.36 +0.4 5,673,443 - PP_4980 0.36 -2.4 5,673,443 - PP_4980 0.36 +1.3
Or see this region's nucleotide sequence