Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0832 and PP_0833 are separated by 14 nucleotides PP_0833 and PP_0834 are separated by 42 nucleotides PP_0834 and PP_0835 are separated by 63 nucleotides
PP_0832: PP_0832 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 969,088 to 970,137
_0832
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645
_0834
PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571
_0835
Position (kb) 970
971
972 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 969.232 kb on + strand, within PP_0832 at 969.232 kb on + strand, within PP_0832 at 969.233 kb on - strand, within PP_0832 at 969.395 kb on + strand, within PP_0832 at 969.396 kb on - strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.531 kb on + strand, within PP_0832 at 969.532 kb on - strand, within PP_0832 at 969.569 kb on - strand, within PP_0832 at 969.753 kb on + strand, within PP_0832 at 970.037 kb on - strand at 970.076 kb on - strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.435 kb on + strand, within PP_0833 at 970.567 kb on + strand, within PP_0833 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833 at 971.201 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 969,232 + PP_0832 0.14 -1.0 969,232 + PP_0832 0.14 -0.9 969,233 - PP_0832 0.14 +1.3 969,395 + PP_0832 0.29 +1.7 969,396 - PP_0832 0.29 +1.3 969,529 + PP_0832 0.42 -2.4 969,529 + PP_0832 0.42 +0.2 969,529 + PP_0832 0.42 -2.8 969,531 + PP_0832 0.42 -2.3 969,532 - PP_0832 0.42 -0.0 969,569 - PP_0832 0.46 +2.3 969,753 + PP_0832 0.63 -0.7 970,037 - +1.3 970,076 - +0.7 970,254 + +2.3 970,254 + -0.3 970,254 + +2.3 970,255 - +5.5 970,255 - +1.3 970,255 - +0.7 970,435 + PP_0833 0.25 -0.3 970,567 + PP_0833 0.37 +2.3 970,716 + PP_0833 0.51 -0.0 970,955 + PP_0833 0.72 +2.3 970,985 + PP_0833 0.75 +0.3 971,060 + PP_0833 0.81 -0.7 971,085 + PP_0833 0.84 +1.3 971,135 + PP_0833 0.88 +4.3 971,201 + +0.7 971,298 + -1.0 971,298 + -3.3 971,298 + +2.1 971,418 + PP_0834 0.32 -3.0 971,418 + PP_0834 0.32 -2.6 971,418 + PP_0834 0.32 -2.9 971,639 + -2.4
Or see this region's nucleotide sequence