Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1345 and PP_1346 are separated by 182 nucleotides PP_1346 and PP_1347 are separated by 155 nucleotides PP_1347 and PP_1348 overlap by 4 nucleotides
PP_1345: PP_1345 - protein translocation complex, ATPase subunit SecA, at 1,531,990 to 1,534,809
_1345
PP_1346: PP_1346 - Glutamate N-acetyltransferase/Amino-acid acetyltransferase, at 1,534,992 to 1,536,209
_1346
PP_1347: PP_1347 - Glutathione S-transferase family protein, at 1,536,365 to 1,536,997
_1347
PP_1348: PP_1348 - putative MutT/nudix family protein/thiamine-phosphate pyrophosphorylase, at 1,536,994 to 1,537,938
_1348
Position (kb) 1534
1535
1536
1537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1534.559 kb on + strand at 1534.559 kb on + strand at 1534.700 kb on + strand at 1534.819 kb on + strand at 1534.819 kb on + strand at 1534.819 kb on + strand at 1534.820 kb on - strand at 1534.820 kb on - strand at 1534.827 kb on - strand at 1534.827 kb on - strand at 1534.980 kb on - strand at 1534.980 kb on - strand at 1534.990 kb on + strand at 1534.990 kb on + strand at 1534.990 kb on + strand at 1534.990 kb on + strand at 1535.188 kb on + strand, within PP_1346 at 1535.188 kb on + strand, within PP_1346 at 1535.189 kb on - strand, within PP_1346 at 1535.206 kb on - strand, within PP_1346 at 1535.603 kb on - strand, within PP_1346 at 1536.016 kb on - strand, within PP_1346 at 1536.063 kb on + strand, within PP_1346 at 1536.064 kb on - strand, within PP_1346 at 1536.064 kb on - strand, within PP_1346 at 1536.064 kb on - strand, within PP_1346 at 1536.066 kb on - strand, within PP_1346 at 1536.177 kb on + strand at 1536.177 kb on + strand at 1536.178 kb on - strand at 1536.203 kb on + strand at 1536.204 kb on - strand at 1536.204 kb on - strand at 1536.204 kb on - strand at 1536.204 kb on - strand at 1536.212 kb on + strand at 1536.212 kb on + strand at 1536.213 kb on - strand at 1536.290 kb on + strand at 1536.371 kb on + strand at 1536.371 kb on + strand at 1536.372 kb on - strand at 1536.372 kb on - strand at 1536.372 kb on - strand at 1536.398 kb on + strand at 1536.399 kb on - strand at 1536.453 kb on - strand, within PP_1347 at 1536.453 kb on - strand, within PP_1347 at 1536.773 kb on - strand, within PP_1347 at 1536.898 kb on - strand, within PP_1347 at 1536.923 kb on + strand, within PP_1347 at 1536.923 kb on + strand, within PP_1347 at 1536.924 kb on - strand, within PP_1347 at 1536.924 kb on - strand, within PP_1347 at 1536.924 kb on - strand, within PP_1347 at 1536.924 kb on - strand, within PP_1347 at 1536.924 kb on - strand, within PP_1347 at 1536.938 kb on + strand at 1536.939 kb on - strand at 1536.939 kb on - strand at 1536.988 kb on + strand at 1536.989 kb on - strand at 1536.989 kb on - strand at 1537.010 kb on + strand at 1537.010 kb on + strand at 1537.011 kb on - strand at 1537.011 kb on - strand at 1537.013 kb on + strand at 1537.178 kb on + strand, within PP_1348
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 1,534,559 + -2.0 1,534,559 + +1.5 1,534,700 + +1.9 1,534,819 + -1.8 1,534,819 + +0.8 1,534,819 + -0.6 1,534,820 - +0.0 1,534,820 - -2.1 1,534,827 - -1.2 1,534,827 - +1.9 1,534,980 - -0.1 1,534,980 - +0.2 1,534,990 + -0.2 1,534,990 + -0.4 1,534,990 + -0.3 1,534,990 + -2.5 1,535,188 + PP_1346 0.16 +1.4 1,535,188 + PP_1346 0.16 -0.1 1,535,189 - PP_1346 0.16 -3.6 1,535,206 - PP_1346 0.18 -2.7 1,535,603 - PP_1346 0.50 -2.4 1,536,016 - PP_1346 0.84 -3.5 1,536,063 + PP_1346 0.88 -2.6 1,536,064 - PP_1346 0.88 +0.4 1,536,064 - PP_1346 0.88 -0.3 1,536,064 - PP_1346 0.88 -3.6 1,536,066 - PP_1346 0.88 -3.0 1,536,177 + -2.5 1,536,177 + +1.1 1,536,178 - +0.5 1,536,203 + -0.7 1,536,204 - +0.5 1,536,204 - +2.4 1,536,204 - -0.4 1,536,204 - +0.1 1,536,212 + -3.5 1,536,212 + -1.5 1,536,213 - -2.6 1,536,290 + -4.1 1,536,371 + -2.5 1,536,371 + -0.4 1,536,372 - -0.2 1,536,372 - -2.5 1,536,372 - +0.3 1,536,398 + +0.5 1,536,399 - +0.3 1,536,453 - PP_1347 0.14 -1.2 1,536,453 - PP_1347 0.14 -0.5 1,536,773 - PP_1347 0.64 -1.2 1,536,898 - PP_1347 0.84 -1.3 1,536,923 + PP_1347 0.88 +0.7 1,536,923 + PP_1347 0.88 +0.1 1,536,924 - PP_1347 0.88 +0.3 1,536,924 - PP_1347 0.88 -0.0 1,536,924 - PP_1347 0.88 +2.1 1,536,924 - PP_1347 0.88 -1.9 1,536,924 - PP_1347 0.88 -0.4 1,536,938 + -2.7 1,536,939 - +2.4 1,536,939 - -0.2 1,536,988 + +1.1 1,536,989 - +0.1 1,536,989 - +0.1 1,537,010 + +1.5 1,537,010 + +0.1 1,537,011 - +2.1 1,537,011 - -0.1 1,537,013 + +1.5 1,537,178 + PP_1348 0.19 +0.5
Or see this region's nucleotide sequence