Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0704 and PP_0705 are separated by 171 nucleotides PP_0705 and PP_0706 are separated by 47 nucleotides PP_0706 and PP_0707 are separated by 56 nucleotides
PP_0704: PP_0704 - RNA polymerase sigma-70 factor, ECF subfamily, at 818,160 to 818,675
_0704
PP_0705: PP_0705 - DNA-3-methyladenine glycosylase, at 818,847 to 819,473
_0705
PP_0706: PP_0706 - fused O(6)-alkylguanine DNA alkyltransferase - transcriptional activator, at 819,521 to 820,573
_0706
PP_0707: PP_0707 - putative membrane protein, at 820,630 to 823,041
_0707
Position (kb) 819
820
821 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 818.546 kb on + strand, within PP_0704 at 818.615 kb on + strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.630 kb on - strand at 818.678 kb on + strand at 818.679 kb on - strand at 818.742 kb on + strand at 818.743 kb on - strand at 818.743 kb on - strand at 818.770 kb on + strand at 818.770 kb on + strand at 818.771 kb on - strand at 818.812 kb on + strand at 818.878 kb on - strand at 818.930 kb on + strand, within PP_0705 at 818.964 kb on - strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 819.303 kb on + strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.456 kb on + strand at 819.457 kb on - strand at 819.457 kb on - strand at 819.491 kb on + strand at 819.491 kb on + strand at 819.506 kb on - strand at 819.530 kb on + strand at 819.530 kb on + strand at 819.606 kb on + strand at 819.606 kb on + strand at 819.606 kb on + strand at 819.607 kb on - strand at 819.610 kb on + strand at 819.610 kb on + strand at 819.610 kb on + strand at 819.611 kb on - strand at 819.611 kb on - strand at 819.661 kb on + strand, within PP_0706 at 819.662 kb on - strand, within PP_0706 at 819.677 kb on + strand, within PP_0706 at 819.730 kb on + strand, within PP_0706 at 819.731 kb on - strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.974 kb on - strand, within PP_0706 at 819.974 kb on - strand, within PP_0706 at 819.990 kb on - strand, within PP_0706 at 820.010 kb on + strand, within PP_0706 at 820.010 kb on + strand, within PP_0706 at 820.011 kb on - strand, within PP_0706 at 820.011 kb on - strand, within PP_0706 at 820.116 kb on + strand, within PP_0706 at 820.117 kb on - strand, within PP_0706 at 820.117 kb on - strand, within PP_0706 at 820.239 kb on - strand, within PP_0706 at 820.382 kb on + strand, within PP_0706 at 820.383 kb on - strand, within PP_0706 at 820.383 kb on - strand, within PP_0706 at 820.456 kb on + strand, within PP_0706 at 820.457 kb on - strand, within PP_0706 at 820.457 kb on - strand, within PP_0706 at 820.625 kb on - strand at 821.037 kb on - strand, within PP_0707 at 821.204 kb on + strand, within PP_0707 at 821.324 kb on + strand, within PP_0707 at 821.325 kb on - strand, within PP_0707 at 821.527 kb on - strand, within PP_0707
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 818,546 + PP_0704 0.75 +0.5 818,615 + PP_0704 0.88 -0.1 818,616 - PP_0704 0.88 -0.6 818,616 - PP_0704 0.88 -0.5 818,630 - -2.8 818,678 + +0.7 818,679 - -1.8 818,742 + +1.6 818,743 - +0.4 818,743 - -0.2 818,770 + -0.2 818,770 + +0.7 818,771 - +0.2 818,812 + -0.5 818,878 - +0.3 818,930 + PP_0705 0.13 -1.1 818,964 - PP_0705 0.19 -2.3 818,982 + PP_0705 0.22 -1.4 818,982 + PP_0705 0.22 -1.6 818,982 + PP_0705 0.22 -0.1 818,983 - PP_0705 0.22 -0.0 818,983 - PP_0705 0.22 -1.7 818,983 - PP_0705 0.22 -0.2 819,303 + PP_0705 0.73 -1.8 819,304 - PP_0705 0.73 -2.9 819,304 - PP_0705 0.73 -2.2 819,304 - PP_0705 0.73 +0.9 819,456 + -2.7 819,457 - -0.7 819,457 - -0.2 819,491 + +0.0 819,491 + -0.4 819,506 - +0.2 819,530 + -0.1 819,530 + -1.1 819,606 + -0.6 819,606 + -1.5 819,606 + +0.4 819,607 - +1.4 819,610 + -2.1 819,610 + -3.3 819,610 + +1.4 819,611 - -0.6 819,611 - +1.1 819,661 + PP_0706 0.13 +0.5 819,662 - PP_0706 0.13 +0.3 819,677 + PP_0706 0.15 -1.5 819,730 + PP_0706 0.20 -0.0 819,731 - PP_0706 0.20 -1.6 819,973 + PP_0706 0.43 +0.8 819,973 + PP_0706 0.43 +0.7 819,973 + PP_0706 0.43 -0.2 819,974 - PP_0706 0.43 +0.3 819,974 - PP_0706 0.43 -1.2 819,990 - PP_0706 0.45 -3.3 820,010 + PP_0706 0.46 -0.5 820,010 + PP_0706 0.46 -1.9 820,011 - PP_0706 0.47 -0.9 820,011 - PP_0706 0.47 -0.6 820,116 + PP_0706 0.57 -3.5 820,117 - PP_0706 0.57 +0.3 820,117 - PP_0706 0.57 -1.2 820,239 - PP_0706 0.68 +0.5 820,382 + PP_0706 0.82 -0.7 820,383 - PP_0706 0.82 -2.1 820,383 - PP_0706 0.82 -1.4 820,456 + PP_0706 0.89 -0.9 820,457 - PP_0706 0.89 -3.0 820,457 - PP_0706 0.89 -0.2 820,625 - -2.7 821,037 - PP_0707 0.17 +0.2 821,204 + PP_0707 0.24 +1.8 821,324 + PP_0707 0.29 -0.0 821,325 - PP_0707 0.29 -1.7 821,527 - PP_0707 0.37 -0.8
Or see this region's nucleotide sequence