Strain Fitness in Pseudomonas putida KT2440 around PP_4822

Experiment: Growth at pH6 and (C) Trisodium citrate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4820 and PP_4821 overlap by 4 nucleotidesPP_4821 and PP_4822 are separated by 81 nucleotidesPP_4822 and PP_4823 are separated by 146 nucleotides PP_4820: PP_4820 - tRNA-dihydrouridine synthase B, at 5,482,075 to 5,483,088 _4820 PP_4821: PP_4821 - putative Fis-like DNA-binding protein, at 5,483,085 to 5,483,405 _4821 PP_4822: PP_4822 - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 5,483,487 to 5,485,094 _4822 PP_4823: PP_4823 - Phosphoribosylamine--glycine ligase, at 5,485,241 to 5,486,536 _4823 Position (kb) 5483 5484 5485 5486Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 5482.630 kb on + strand, within PP_4820at 5482.801 kb on + strandat 5482.891 kb on + strand, within PP_4820at 5482.891 kb on + strand, within PP_4820at 5482.891 kb on + strand, within PP_4820at 5482.891 kb on + strand, within PP_4820at 5482.891 kb on + strand, within PP_4820at 5482.910 kb on + strand, within PP_4820at 5482.910 kb on + strand, within PP_4820at 5485.149 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate
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5,482,630 + PP_4820 0.55 +0.8
5,482,801 + +2.0
5,482,891 + PP_4820 0.80 +1.2
5,482,891 + PP_4820 0.80 -2.0
5,482,891 + PP_4820 0.80 +0.5
5,482,891 + PP_4820 0.80 +0.6
5,482,891 + PP_4820 0.80 +0.9
5,482,910 + PP_4820 0.82 -2.0
5,482,910 + PP_4820 0.82 -0.9
5,485,149 + -5.2

Or see this region's nucleotide sequence