Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4551 and PP_4552 are separated by 34 nucleotides PP_4552 and PP_4553 are separated by 116 nucleotides PP_4553 and PP_4554 are separated by 17 nucleotides PP_4554 and PP_4555 are separated by 21 nucleotides PP_4555 and PP_4556 are separated by 44 nucleotides
PP_4551: PP_4551 - Hydrolase, alpha/beta fold family, at 5,175,198 to 5,176,142
_4551
PP_4552: PP_4552 - putative conserved enzyme of unknown function, at 5,176,177 to 5,176,647
_4552
PP_4553: PP_4553 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,176,764 to 5,177,984
_4553
PP_4554: PP_4554 - conserved protein of unknown function, at 5,178,002 to 5,178,487
_4554
PP_4555: PP_4555 - conserved protein of unknown function, at 5,178,509 to 5,178,853
_4555
PP_4556: PP_4556 - conserved protein of unknown function, at 5,178,898 to 5,179,323
_4556
Position (kb) 5176
5177
5178 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5175.798 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.026 kb on + strand, within PP_4551 at 5176.048 kb on + strand at 5176.712 kb on + strand at 5176.794 kb on - strand at 5176.913 kb on - strand, within PP_4553 at 5176.913 kb on - strand, within PP_4553 at 5176.913 kb on - strand, within PP_4553 at 5177.047 kb on + strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.129 kb on + strand, within PP_4553 at 5177.130 kb on - strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553 at 5177.211 kb on - strand, within PP_4553 at 5177.211 kb on - strand, within PP_4553 at 5177.408 kb on - strand, within PP_4553 at 5177.428 kb on + strand, within PP_4553 at 5177.428 kb on + strand, within PP_4553 at 5177.429 kb on - strand, within PP_4553 at 5177.481 kb on + strand, within PP_4553 at 5177.489 kb on - strand, within PP_4553 at 5177.493 kb on + strand, within PP_4553 at 5177.494 kb on - strand, within PP_4553 at 5177.531 kb on + strand, within PP_4553 at 5177.938 kb on - strand at 5178.411 kb on - strand, within PP_4554 at 5178.548 kb on - strand, within PP_4555 at 5178.548 kb on - strand, within PP_4555 at 5178.548 kb on - strand, within PP_4555 at 5178.618 kb on + strand, within PP_4555 at 5178.692 kb on + strand, within PP_4555 at 5178.831 kb on + strand at 5178.831 kb on + strand at 5178.832 kb on - strand at 5178.832 kb on - strand at 5178.832 kb on - strand at 5178.846 kb on + strand at 5178.846 kb on + strand at 5178.847 kb on - strand at 5178.847 kb on - strand at 5178.869 kb on - strand at 5178.967 kb on + strand, within PP_4556 at 5178.967 kb on + strand, within PP_4556 at 5178.968 kb on - strand, within PP_4556 at 5178.968 kb on - strand, within PP_4556
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 5,175,798 + PP_4551 0.63 -0.4 5,176,012 + PP_4551 0.86 +0.7 5,176,012 + PP_4551 0.86 +1.9 5,176,012 + PP_4551 0.86 +0.3 5,176,012 + PP_4551 0.86 +2.2 5,176,012 + PP_4551 0.86 +1.4 5,176,013 - PP_4551 0.86 +1.0 5,176,013 - PP_4551 0.86 +1.7 5,176,013 - PP_4551 0.86 +1.6 5,176,013 - PP_4551 0.86 -0.9 5,176,013 - PP_4551 0.86 +1.6 5,176,013 - PP_4551 0.86 +1.8 5,176,013 - PP_4551 0.86 +2.3 5,176,013 - PP_4551 0.86 +0.3 5,176,013 - PP_4551 0.86 +2.3 5,176,026 + PP_4551 0.88 -3.4 5,176,048 + -0.4 5,176,712 + +0.2 5,176,794 - +0.7 5,176,913 - PP_4553 0.12 +0.0 5,176,913 - PP_4553 0.12 +0.8 5,176,913 - PP_4553 0.12 +0.9 5,177,047 + PP_4553 0.23 +0.2 5,177,048 - PP_4553 0.23 +1.5 5,177,048 - PP_4553 0.23 +0.9 5,177,048 - PP_4553 0.23 +2.3 5,177,129 + PP_4553 0.30 -2.4 5,177,130 - PP_4553 0.30 +0.6 5,177,131 + PP_4553 0.30 +0.5 5,177,131 + PP_4553 0.30 -0.6 5,177,131 + PP_4553 0.30 -0.3 5,177,132 - PP_4553 0.30 -0.6 5,177,132 - PP_4553 0.30 -2.8 5,177,211 - PP_4553 0.37 -0.1 5,177,211 - PP_4553 0.37 +1.3 5,177,408 - PP_4553 0.53 -0.6 5,177,428 + PP_4553 0.54 +0.1 5,177,428 + PP_4553 0.54 +0.0 5,177,429 - PP_4553 0.54 +0.2 5,177,481 + PP_4553 0.59 +0.8 5,177,489 - PP_4553 0.59 +0.4 5,177,493 + PP_4553 0.60 +0.5 5,177,494 - PP_4553 0.60 +1.1 5,177,531 + PP_4553 0.63 -1.3 5,177,938 - +0.9 5,178,411 - PP_4554 0.84 +0.3 5,178,548 - PP_4555 0.11 -0.0 5,178,548 - PP_4555 0.11 -2.7 5,178,548 - PP_4555 0.11 -0.3 5,178,618 + PP_4555 0.32 -0.0 5,178,692 + PP_4555 0.53 +0.6 5,178,831 + +1.4 5,178,831 + +0.8 5,178,832 - -2.0 5,178,832 - -1.7 5,178,832 - -0.3 5,178,846 + +2.2 5,178,846 + +0.5 5,178,847 - +0.2 5,178,847 - +1.3 5,178,869 - -0.0 5,178,967 + PP_4556 0.16 -3.1 5,178,967 + PP_4556 0.16 -3.0 5,178,968 - PP_4556 0.16 -2.6 5,178,968 - PP_4556 0.16 -2.6
Or see this region's nucleotide sequence