Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4532 and PP_4533 are separated by 142 nucleotides PP_4533 and PP_5695 are separated by 72 nucleotides PP_5695 and PP_4534 are separated by 95 nucleotides
PP_4532: PP_4532 - ATP-dependent DEAD-box RNA helicase require for 50S ribosomal subunit biogenesis, at 5,145,431 to 5,146,753
_4532
PP_4533: PP_4533 - mechanosensitive channel of miniconductance, at 5,146,896 to 5,148,197
_4533
PP_5695: PP_5695 - conserved protein of unknown function, at 5,148,270 to 5,148,539
_5695
PP_4534: PP_4534 - conserved protein of unknown function, at 5,148,635 to 5,148,877
_4534
Position (kb) 5146
5147
5148
5149 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5146.793 kb on - strand at 5146.856 kb on + strand at 5146.856 kb on + strand at 5146.857 kb on - strand at 5146.857 kb on - strand at 5146.886 kb on - strand at 5146.886 kb on - strand at 5146.886 kb on - strand at 5146.886 kb on - strand at 5147.008 kb on + strand at 5147.009 kb on - strand at 5147.009 kb on - strand at 5147.036 kb on - strand, within PP_4533 at 5147.223 kb on - strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.280 kb on + strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.281 kb on - strand, within PP_4533 at 5147.322 kb on + strand, within PP_4533 at 5147.323 kb on - strand, within PP_4533 at 5147.323 kb on - strand, within PP_4533 at 5147.437 kb on + strand, within PP_4533 at 5147.438 kb on - strand, within PP_4533 at 5147.451 kb on + strand, within PP_4533 at 5147.452 kb on - strand, within PP_4533 at 5147.452 kb on - strand, within PP_4533 at 5147.542 kb on + strand, within PP_4533 at 5147.542 kb on + strand, within PP_4533 at 5147.543 kb on - strand, within PP_4533 at 5147.543 kb on - strand, within PP_4533 at 5147.997 kb on + strand, within PP_4533 at 5148.061 kb on - strand, within PP_4533 at 5148.226 kb on - strand at 5148.271 kb on + strand at 5148.272 kb on - strand at 5148.272 kb on - strand at 5148.334 kb on + strand, within PP_5695 at 5148.334 kb on + strand, within PP_5695 at 5148.351 kb on + strand, within PP_5695 at 5148.351 kb on + strand, within PP_5695 at 5148.351 kb on + strand, within PP_5695 at 5148.400 kb on + strand, within PP_5695 at 5148.493 kb on + strand, within PP_5695 at 5148.493 kb on + strand, within PP_5695 at 5148.493 kb on + strand, within PP_5695 at 5148.494 kb on - strand, within PP_5695 at 5148.494 kb on - strand, within PP_5695 at 5148.547 kb on + strand at 5148.547 kb on + strand at 5148.547 kb on + strand at 5148.547 kb on + strand at 5148.548 kb on - strand at 5148.548 kb on - strand at 5148.548 kb on - strand at 5148.548 kb on - strand at 5148.548 kb on - strand at 5148.805 kb on - strand, within PP_4534 at 5148.805 kb on - strand, within PP_4534 at 5148.805 kb on - strand, within PP_4534 at 5148.849 kb on + strand, within PP_4534 at 5148.849 kb on + strand, within PP_4534 at 5148.850 kb on - strand, within PP_4534 at 5148.850 kb on - strand, within PP_4534 at 5148.882 kb on + strand at 5148.882 kb on + strand at 5148.883 kb on - strand at 5148.883 kb on - strand at 5148.907 kb on - strand at 5149.019 kb on + strand at 5149.020 kb on - strand at 5149.020 kb on - strand at 5149.020 kb on - strand at 5149.020 kb on - strand at 5149.020 kb on - strand at 5149.020 kb on - strand at 5149.025 kb on + strand at 5149.025 kb on + strand at 5149.025 kb on + strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.026 kb on - strand at 5149.089 kb on + strand at 5149.090 kb on - strand at 5149.124 kb on + strand at 5149.124 kb on + strand at 5149.125 kb on - strand at 5149.125 kb on - strand at 5149.125 kb on - strand at 5149.142 kb on + strand at 5149.143 kb on - strand at 5149.194 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 5,146,793 - -3.7 5,146,856 + -0.2 5,146,856 + +0.8 5,146,857 - +1.3 5,146,857 - -0.1 5,146,886 - -1.3 5,146,886 - -0.4 5,146,886 - +0.3 5,146,886 - +1.1 5,147,008 + +0.2 5,147,009 - +0.0 5,147,009 - -1.2 5,147,036 - PP_4533 0.11 -2.4 5,147,223 - PP_4533 0.25 -0.7 5,147,280 + PP_4533 0.29 +0.1 5,147,280 + PP_4533 0.29 +1.4 5,147,280 + PP_4533 0.29 -2.1 5,147,280 + PP_4533 0.29 -0.2 5,147,280 + PP_4533 0.29 -0.2 5,147,280 + PP_4533 0.29 -0.5 5,147,281 - PP_4533 0.30 -0.1 5,147,281 - PP_4533 0.30 -0.7 5,147,281 - PP_4533 0.30 -0.1 5,147,281 - PP_4533 0.30 -1.7 5,147,281 - PP_4533 0.30 -0.2 5,147,281 - PP_4533 0.30 -1.2 5,147,281 - PP_4533 0.30 +0.3 5,147,281 - PP_4533 0.30 -3.0 5,147,281 - PP_4533 0.30 +1.4 5,147,322 + PP_4533 0.33 -0.2 5,147,323 - PP_4533 0.33 +0.5 5,147,323 - PP_4533 0.33 -1.7 5,147,437 + PP_4533 0.42 -0.3 5,147,438 - PP_4533 0.42 -3.6 5,147,451 + PP_4533 0.43 +2.7 5,147,452 - PP_4533 0.43 +1.6 5,147,452 - PP_4533 0.43 -0.8 5,147,542 + PP_4533 0.50 -1.5 5,147,542 + PP_4533 0.50 -0.0 5,147,543 - PP_4533 0.50 +0.0 5,147,543 - PP_4533 0.50 -1.2 5,147,997 + PP_4533 0.85 +0.3 5,148,061 - PP_4533 0.89 -1.6 5,148,226 - -0.7 5,148,271 + +1.2 5,148,272 - -3.7 5,148,272 - +0.2 5,148,334 + PP_5695 0.24 +0.5 5,148,334 + PP_5695 0.24 +2.0 5,148,351 + PP_5695 0.30 -0.3 5,148,351 + PP_5695 0.30 +0.2 5,148,351 + PP_5695 0.30 -0.0 5,148,400 + PP_5695 0.48 +1.7 5,148,493 + PP_5695 0.83 +1.2 5,148,493 + PP_5695 0.83 -1.3 5,148,493 + PP_5695 0.83 +1.5 5,148,494 - PP_5695 0.83 -0.9 5,148,494 - PP_5695 0.83 +1.0 5,148,547 + +0.7 5,148,547 + -3.0 5,148,547 + +0.2 5,148,547 + -0.7 5,148,548 - -0.6 5,148,548 - +0.8 5,148,548 - +0.4 5,148,548 - -0.1 5,148,548 - +1.0 5,148,805 - PP_4534 0.70 +0.5 5,148,805 - PP_4534 0.70 -3.8 5,148,805 - PP_4534 0.70 +0.2 5,148,849 + PP_4534 0.88 -2.7 5,148,849 + PP_4534 0.88 -0.9 5,148,850 - PP_4534 0.88 +0.2 5,148,850 - PP_4534 0.88 +1.2 5,148,882 + +0.9 5,148,882 + +0.2 5,148,883 - -0.9 5,148,883 - -1.8 5,148,907 - -1.4 5,149,019 + -0.8 5,149,020 - -3.6 5,149,020 - -0.1 5,149,020 - +0.5 5,149,020 - +0.5 5,149,020 - +1.9 5,149,020 - +0.5 5,149,025 + +0.6 5,149,025 + +2.2 5,149,025 + +0.9 5,149,026 - -0.0 5,149,026 - -1.9 5,149,026 - -0.1 5,149,026 - +1.6 5,149,026 - +1.6 5,149,026 - +1.3 5,149,026 - -0.3 5,149,026 - +1.3 5,149,089 + -0.9 5,149,090 - +2.0 5,149,124 + +0.5 5,149,124 + -0.5 5,149,125 - -3.8 5,149,125 - -0.7 5,149,125 - +0.6 5,149,142 + +0.8 5,149,143 - -0.0 5,149,194 + +0.6
Or see this region's nucleotide sequence