Strain Fitness in Pseudomonas putida KT2440 around PP_3281

Experiment: Growth at pH6 and (C) Trisodium citrate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_3280 and PP_3281 overlap by 8 nucleotidesPP_3281 and PP_3282 overlap by 8 nucleotides PP_3280: PP_3280 - 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, at 3,714,635 to 3,715,855 _3280 PP_3281: PP_3281 - hydroxyphenylacetyl-CoA thioesterase, at 3,715,848 to 3,716,288 _3281 PP_3282: PP_3282 - 3-hydroxyadipyl-CoA dehydrogenase, at 3,716,281 to 3,717,798 _3282 Position (kb) 3715 3716 3717Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3715.097 kb on + strand, within PP_3280at 3715.457 kb on - strand, within PP_3280at 3715.599 kb on - strand, within PP_3280at 3715.684 kb on + strand, within PP_3280at 3715.684 kb on + strand, within PP_3280at 3715.684 kb on + strand, within PP_3280at 3715.685 kb on - strand, within PP_3280at 3715.895 kb on - strand, within PP_3281at 3715.954 kb on + strand, within PP_3281at 3715.955 kb on - strand, within PP_3281at 3715.955 kb on - strand, within PP_3281at 3716.032 kb on + strand, within PP_3281at 3716.033 kb on - strand, within PP_3281at 3716.072 kb on - strand, within PP_3281at 3716.072 kb on - strand, within PP_3281at 3716.075 kb on + strand, within PP_3281at 3716.075 kb on + strand, within PP_3281at 3716.075 kb on + strand, within PP_3281at 3716.076 kb on - strand, within PP_3281at 3716.164 kb on - strand, within PP_3281at 3716.290 kb on + strandat 3716.290 kb on + strandat 3716.291 kb on - strandat 3716.291 kb on - strandat 3716.291 kb on - strandat 3716.291 kb on - strandat 3716.436 kb on - strand, within PP_3282at 3716.673 kb on - strand, within PP_3282at 3716.940 kb on - strand, within PP_3282at 3716.945 kb on + strand, within PP_3282at 3716.945 kb on + strand, within PP_3282at 3716.946 kb on - strand, within PP_3282at 3716.946 kb on - strand, within PP_3282at 3716.946 kb on - strand, within PP_3282at 3717.047 kb on - strand, within PP_3282at 3717.065 kb on + strand, within PP_3282at 3717.065 kb on + strand, within PP_3282at 3717.065 kb on + strand, within PP_3282at 3717.065 kb on + strand, within PP_3282at 3717.065 kb on + strand, within PP_3282at 3717.066 kb on - strand, within PP_3282at 3717.066 kb on - strand, within PP_3282at 3717.066 kb on - strand, within PP_3282at 3717.066 kb on - strand, within PP_3282at 3717.184 kb on + strand, within PP_3282at 3717.185 kb on - strand, within PP_3282at 3717.198 kb on - strand, within PP_3282at 3717.198 kb on - strandat 3717.238 kb on - strand, within PP_3282at 3717.284 kb on + strand, within PP_3282at 3717.285 kb on - strand, within PP_3282at 3717.285 kb on - strand, within PP_3282

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate
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3,715,097 + PP_3280 0.38 -1.7
3,715,457 - PP_3280 0.67 -0.8
3,715,599 - PP_3280 0.79 -0.1
3,715,684 + PP_3280 0.86 -0.2
3,715,684 + PP_3280 0.86 -4.0
3,715,684 + PP_3280 0.86 -1.1
3,715,685 - PP_3280 0.86 -0.6
3,715,895 - PP_3281 0.11 -0.3
3,715,954 + PP_3281 0.24 -0.1
3,715,955 - PP_3281 0.24 -0.3
3,715,955 - PP_3281 0.24 -0.9
3,716,032 + PP_3281 0.42 -1.5
3,716,033 - PP_3281 0.42 -0.4
3,716,072 - PP_3281 0.51 -1.9
3,716,072 - PP_3281 0.51 -0.1
3,716,075 + PP_3281 0.51 +0.2
3,716,075 + PP_3281 0.51 -2.4
3,716,075 + PP_3281 0.51 +1.1
3,716,076 - PP_3281 0.52 -0.3
3,716,164 - PP_3281 0.72 -4.1
3,716,290 + +0.9
3,716,290 + -0.2
3,716,291 - -0.6
3,716,291 - -0.5
3,716,291 - +0.8
3,716,291 - +0.6
3,716,436 - PP_3282 0.10 +0.3
3,716,673 - PP_3282 0.26 -2.1
3,716,940 - PP_3282 0.43 -3.2
3,716,945 + PP_3282 0.44 -0.7
3,716,945 + PP_3282 0.44 +1.6
3,716,946 - PP_3282 0.44 +1.4
3,716,946 - PP_3282 0.44 +1.8
3,716,946 - PP_3282 0.44 -4.1
3,717,047 - PP_3282 0.50 +1.0
3,717,065 + PP_3282 0.52 -1.2
3,717,065 + PP_3282 0.52 -0.4
3,717,065 + PP_3282 0.52 -3.4
3,717,065 + PP_3282 0.52 -1.8
3,717,065 + PP_3282 0.52 -0.1
3,717,066 - PP_3282 0.52 +0.5
3,717,066 - PP_3282 0.52 +0.0
3,717,066 - PP_3282 0.52 -3.2
3,717,066 - PP_3282 0.52 -1.2
3,717,184 + PP_3282 0.59 +1.4
3,717,185 - PP_3282 0.60 -0.3
3,717,198 - PP_3282 0.60 -0.7
3,717,198 - -1.8
3,717,238 - PP_3282 0.63 +1.0
3,717,284 + PP_3282 0.66 +1.1
3,717,285 - PP_3282 0.66 +0.3
3,717,285 - PP_3282 0.66 -3.0

Or see this region's nucleotide sequence