Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1511 and PP_1512 are separated by 2 nucleotides PP_1512 and PP_1513 are separated by 104 nucleotides PP_1513 and PP_1514 are separated by 192 nucleotides
PP_1511: PP_1511 - conserved protein of unknown function, at 1,715,885 to 1,716,574
_1511
PP_1512: PP_1512 - conserved protein of unknown function, at 1,716,577 to 1,717,197
_1512
PP_1513: PP_1513 - Ribosomal RNA small subunit methyltransferase J, at 1,717,302 to 1,718,078
_1513
PP_1514: PP_1514 - conserved protein of unknown function, at 1,718,271 to 1,719,239
_1514
Position (kb)
1717
1718
1719 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1716.320 kb on + strand, within PP_1511 at 1716.512 kb on + strand at 1717.023 kb on - strand, within PP_1512 at 1717.023 kb on - strand, within PP_1512 at 1717.023 kb on - strand, within PP_1512 at 1717.091 kb on - strand, within PP_1512 at 1717.145 kb on + strand at 1717.146 kb on - strand at 1717.166 kb on + strand at 1717.166 kb on + strand at 1717.167 kb on - strand at 1717.167 kb on - strand at 1717.167 kb on - strand at 1717.188 kb on + strand at 1717.189 kb on - strand at 1717.189 kb on - strand at 1717.189 kb on - strand at 1717.270 kb on - strand at 1717.351 kb on - strand at 1717.351 kb on - strand at 1717.594 kb on + strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1718.019 kb on - strand at 1718.031 kb on - strand at 1718.044 kb on - strand at 1718.052 kb on + strand at 1718.052 kb on + strand at 1718.053 kb on - strand at 1718.087 kb on + strand at 1718.088 kb on - strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.176 kb on + strand at 1718.188 kb on + strand at 1718.188 kb on + strand at 1718.189 kb on - strand at 1718.189 kb on - strand at 1718.202 kb on - strand at 1718.202 kb on - strand at 1718.214 kb on - strand at 1718.214 kb on - strand at 1718.220 kb on + strand at 1718.220 kb on + strand at 1718.255 kb on - strand at 1718.255 kb on - strand at 1718.270 kb on - strand at 1718.292 kb on + strand at 1718.292 kb on + strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.479 kb on - strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.616 kb on + strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.697 kb on + strand, within PP_1514 at 1718.698 kb on - strand at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.756 kb on + strand, within PP_1514 at 1718.809 kb on + strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.828 kb on - strand, within PP_1514 at 1718.870 kb on + strand, within PP_1514 at 1718.871 kb on - strand, within PP_1514 at 1718.876 kb on - strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.923 kb on + strand, within PP_1514 at 1718.924 kb on - strand, within PP_1514 at 1718.972 kb on + strand, within PP_1514
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 1,716,320 + PP_1511 0.63 -1.3 1,716,512 + -3.3 1,717,023 - PP_1512 0.72 -0.7 1,717,023 - PP_1512 0.72 -3.8 1,717,023 - PP_1512 0.72 -1.1 1,717,091 - PP_1512 0.83 -1.4 1,717,145 + +0.6 1,717,146 - +0.5 1,717,166 + +1.9 1,717,166 + -1.2 1,717,167 - -0.0 1,717,167 - +0.4 1,717,167 - -4.1 1,717,188 + +0.5 1,717,189 - -0.0 1,717,189 - +0.2 1,717,189 - +0.7 1,717,270 - +0.6 1,717,351 - +0.7 1,717,351 - -0.9 1,717,594 + PP_1513 0.38 +0.9 1,717,825 - PP_1513 0.67 +1.0 1,717,825 - PP_1513 0.67 +0.6 1,718,019 - -0.6 1,718,031 - -1.6 1,718,044 - +1.0 1,718,052 + +1.4 1,718,052 + +0.1 1,718,053 - +0.1 1,718,087 + +1.0 1,718,088 - +0.0 1,718,110 + +1.0 1,718,110 + +0.1 1,718,110 + +1.7 1,718,111 - +0.7 1,718,111 - +0.4 1,718,111 - -0.6 1,718,111 - -0.0 1,718,176 + +0.5 1,718,188 + +2.0 1,718,188 + +1.0 1,718,189 - -1.2 1,718,189 - -2.1 1,718,202 - -2.7 1,718,202 - -2.2 1,718,214 - -0.3 1,718,214 - +0.9 1,718,220 + -0.6 1,718,220 + -0.8 1,718,255 - +0.9 1,718,255 - -3.6 1,718,270 - -0.1 1,718,292 + +0.9 1,718,292 + +0.5 1,718,339 - +0.5 1,718,339 - +1.5 1,718,339 - +1.3 1,718,479 - PP_1514 0.21 -0.8 1,718,508 + PP_1514 0.24 +0.8 1,718,508 + PP_1514 0.24 +0.1 1,718,509 - PP_1514 0.25 -0.9 1,718,509 - PP_1514 0.25 -0.6 1,718,598 + PP_1514 0.34 -2.2 1,718,598 + PP_1514 0.34 -1.3 1,718,599 - PP_1514 0.34 +0.3 1,718,599 - PP_1514 0.34 +1.7 1,718,616 + PP_1514 0.36 +0.7 1,718,617 - PP_1514 0.36 -0.6 1,718,617 - PP_1514 0.36 +1.6 1,718,697 + PP_1514 0.44 -1.0 1,718,698 - -0.3 1,718,698 - PP_1514 0.44 +2.2 1,718,698 - PP_1514 0.44 +1.0 1,718,698 - PP_1514 0.44 +0.6 1,718,703 + PP_1514 0.45 +0.4 1,718,703 + PP_1514 0.45 +0.7 1,718,756 + PP_1514 0.50 -1.3 1,718,809 + PP_1514 0.56 +0.2 1,718,820 - PP_1514 0.57 +1.0 1,718,820 - PP_1514 0.57 +1.3 1,718,828 - PP_1514 0.57 +0.2 1,718,870 + PP_1514 0.62 +0.5 1,718,871 - PP_1514 0.62 +0.4 1,718,876 - PP_1514 0.62 +0.0 1,718,885 + PP_1514 0.63 -1.0 1,718,885 + PP_1514 0.63 +0.7 1,718,923 + PP_1514 0.67 +0.6 1,718,924 - PP_1514 0.67 +0.3 1,718,972 + PP_1514 0.72 -1.3
Or see this region's nucleotide sequence