Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0409 and PP_0410 are separated by 10 nucleotides PP_0410 and PP_0411 are separated by 295 nucleotides PP_0411 and PP_0412 are separated by 40 nucleotides
PP_0409: PP_0409 - Sensory box histidine kinase, at 496,129 to 498,516
_0409
PP_0410: PP_0410 - DNA-binding response regulator UhpA, at 498,527 to 499,156
_0410
PP_0411: PP_0411 - Polyamine ABC transporter, ATP-binding protein, at 499,452 to 500,576
_0411
PP_0412: PP_0412 - Polyamine ABC transporter, periplasmic polyamine-binding protein, at 500,617 to 501,651
_0412
Position (kb) 499
500
501 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 498.515 kb on - strand at 498.515 kb on - strand at 498.538 kb on - strand at 498.538 kb on - strand at 498.678 kb on + strand, within PP_0410 at 498.678 kb on + strand, within PP_0410 at 498.896 kb on + strand, within PP_0410 at 498.897 kb on - strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.898 kb on + strand, within PP_0410 at 498.899 kb on - strand, within PP_0410 at 498.899 kb on - strand, within PP_0410 at 498.936 kb on + strand, within PP_0410 at 498.937 kb on - strand, within PP_0410 at 498.937 kb on - strand, within PP_0410 at 499.130 kb on + strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.131 kb on - strand at 499.165 kb on + strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.166 kb on - strand at 499.192 kb on + strand at 499.193 kb on - strand at 499.193 kb on - strand at 499.239 kb on + strand at 499.240 kb on - strand at 499.240 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.378 kb on + strand at 499.410 kb on + strand at 499.411 kb on - strand at 499.422 kb on + strand at 499.521 kb on + strand at 499.522 kb on - strand at 499.522 kb on - strand at 499.522 kb on - strand at 499.716 kb on - strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.737 kb on + strand, within PP_0411 at 499.738 kb on - strand, within PP_0411 at 499.951 kb on + strand, within PP_0411 at 499.952 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.053 kb on - strand, within PP_0411 at 500.327 kb on - strand, within PP_0411 at 500.406 kb on + strand, within PP_0411 at 500.407 kb on - strand, within PP_0411 at 500.512 kb on + strand at 500.512 kb on + strand at 500.512 kb on + strand at 500.512 kb on + strand at 500.513 kb on - strand at 500.513 kb on - strand at 500.515 kb on + strand at 500.515 kb on + strand at 500.516 kb on - strand at 500.598 kb on + strand at 500.598 kb on + strand at 500.598 kb on + strand at 500.599 kb on - strand at 500.612 kb on + strand at 500.613 kb on - strand at 500.613 kb on - strand at 500.616 kb on - strand at 500.743 kb on + strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.744 kb on - strand, within PP_0412 at 500.791 kb on + strand, within PP_0412 at 500.792 kb on - strand, within PP_0412 at 500.792 kb on - strand, within PP_0412 at 500.856 kb on - strand, within PP_0412 at 500.944 kb on + strand, within PP_0412 at 500.945 kb on - strand, within PP_0412 at 501.007 kb on + strand, within PP_0412 at 501.008 kb on - strand, within PP_0412 at 501.008 kb on - strand, within PP_0412 at 501.106 kb on + strand, within PP_0412 at 501.107 kb on - strand, within PP_0412 at 501.158 kb on - strand, within PP_0412 at 501.158 kb on - strand, within PP_0412 at 501.298 kb on + strand, within PP_0412 at 501.310 kb on - strand, within PP_0412 at 501.364 kb on + strand, within PP_0412 at 501.365 kb on - strand, within PP_0412 at 501.422 kb on - strand, within PP_0412 at 501.433 kb on + strand, within PP_0412 at 501.463 kb on + strand, within PP_0412 at 501.463 kb on + strand, within PP_0412 at 501.482 kb on - strand, within PP_0412
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 498,515 - +1.5 498,515 - -1.2 498,538 - -1.9 498,538 - +1.1 498,678 + PP_0410 0.24 -1.0 498,678 + PP_0410 0.24 +0.1 498,896 + PP_0410 0.59 -0.3 498,897 - PP_0410 0.59 +1.5 498,898 + PP_0410 0.59 +1.0 498,898 + PP_0410 0.59 -4.2 498,898 + PP_0410 0.59 +0.5 498,899 - PP_0410 0.59 -0.2 498,899 - PP_0410 0.59 +0.1 498,936 + PP_0410 0.65 +1.5 498,937 - PP_0410 0.65 -0.5 498,937 - PP_0410 0.65 -1.2 499,130 + +0.5 499,131 - -3.7 499,131 - +0.5 499,131 - +0.1 499,131 - -0.4 499,131 - -3.3 499,131 - +0.2 499,165 + -3.0 499,166 - -0.2 499,166 - +0.3 499,166 - -1.4 499,166 - +0.1 499,166 - +1.3 499,192 + -3.2 499,193 - +0.9 499,193 - -2.6 499,239 + +0.7 499,240 - +0.1 499,240 - -1.9 499,340 - +0.1 499,340 - -0.6 499,340 - +0.2 499,378 + +0.8 499,410 + +0.5 499,411 - +1.9 499,422 + +0.6 499,521 + +0.9 499,522 - +0.6 499,522 - -0.1 499,522 - -2.3 499,716 - PP_0411 0.23 -1.9 499,737 + PP_0411 0.25 +1.2 499,737 + PP_0411 0.25 -2.4 499,737 + PP_0411 0.25 -0.4 499,738 - PP_0411 0.25 +1.9 499,951 + PP_0411 0.44 +1.1 499,952 - PP_0411 0.44 +0.5 500,053 - PP_0411 0.53 +0.6 500,053 - PP_0411 0.53 +1.2 500,053 - PP_0411 0.53 +2.2 500,327 - PP_0411 0.78 +1.5 500,406 + PP_0411 0.85 +0.7 500,407 - PP_0411 0.85 +0.5 500,512 + -0.3 500,512 + +0.4 500,512 + -2.7 500,512 + +1.7 500,513 - -0.9 500,513 - -0.4 500,515 + +0.5 500,515 + -3.9 500,516 - -1.2 500,598 + -1.7 500,598 + -0.2 500,598 + -3.0 500,599 - +1.0 500,612 + -1.1 500,613 - -0.4 500,613 - +0.5 500,616 - +0.7 500,743 + PP_0412 0.12 -1.4 500,744 - PP_0412 0.12 -0.3 500,744 - PP_0412 0.12 -1.7 500,744 - PP_0412 0.12 +1.0 500,744 - PP_0412 0.12 -0.9 500,791 + PP_0412 0.17 -3.5 500,792 - PP_0412 0.17 +0.7 500,792 - PP_0412 0.17 +1.6 500,856 - PP_0412 0.23 +0.8 500,944 + PP_0412 0.32 +0.5 500,945 - PP_0412 0.32 +0.3 501,007 + PP_0412 0.38 -0.6 501,008 - PP_0412 0.38 -3.3 501,008 - PP_0412 0.38 +1.8 501,106 + PP_0412 0.47 +0.9 501,107 - PP_0412 0.47 -1.9 501,158 - PP_0412 0.52 +0.4 501,158 - PP_0412 0.52 +0.4 501,298 + PP_0412 0.66 +1.0 501,310 - PP_0412 0.67 -1.7 501,364 + PP_0412 0.72 +0.5 501,365 - PP_0412 0.72 +2.1 501,422 - PP_0412 0.78 +3.0 501,433 + PP_0412 0.79 -0.8 501,463 + PP_0412 0.82 -1.8 501,463 + PP_0412 0.82 +0.1 501,482 - PP_0412 0.84 +0.5
Or see this region's nucleotide sequence