Experiment: Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23440 and PS417_23445 overlap by 1 nucleotides PS417_23445 and PS417_23450 are separated by 149 nucleotides PS417_23450 and PS417_23455 overlap by 11 nucleotides PS417_23455 and PS417_23460 are separated by 98 nucleotides
PS417_23440: PS417_23440 - alpha/beta hydrolase, at 5,077,754 to 5,078,659
_23440
PS417_23445: PS417_23445 - aldolase, at 5,078,659 to 5,079,441
_23445
PS417_23450: PS417_23450 - membrane protein, at 5,079,591 to 5,080,277
_23450
PS417_23455: PS417_23455 - LrgA, at 5,080,267 to 5,080,638
_23455
PS417_23460: PS417_23460 - LysR family transcriptional regulator, at 5,080,737 to 5,081,633
_23460
Position (kb)
5079
5080
5081 Strain fitness (log2 ratio)
-2
-1
0
1 at 5078.667 kb on + strand at 5078.670 kb on - strand at 5079.115 kb on + strand, within PS417_23445 at 5079.115 kb on + strand, within PS417_23445 at 5079.115 kb on + strand at 5079.153 kb on + strand at 5079.153 kb on + strand, within PS417_23445 at 5079.153 kb on + strand, within PS417_23445 at 5079.154 kb on - strand, within PS417_23445 at 5079.155 kb on + strand, within PS417_23445 at 5079.176 kb on + strand, within PS417_23445 at 5079.176 kb on + strand, within PS417_23445 at 5079.177 kb on - strand, within PS417_23445 at 5079.303 kb on + strand, within PS417_23445 at 5079.497 kb on - strand at 5079.497 kb on - strand at 5079.497 kb on - strand at 5079.567 kb on + strand at 5080.109 kb on - strand, within PS417_23450 at 5080.152 kb on + strand, within PS417_23450 at 5080.153 kb on - strand, within PS417_23450 at 5080.182 kb on + strand, within PS417_23450 at 5080.182 kb on + strand, within PS417_23450 at 5080.182 kb on + strand, within PS417_23450 at 5080.183 kb on - strand, within PS417_23450 at 5080.183 kb on - strand, within PS417_23450 at 5080.183 kb on - strand, within PS417_23450 at 5080.223 kb on + strand at 5080.223 kb on + strand at 5080.223 kb on + strand at 5080.225 kb on + strand at 5080.225 kb on + strand at 5080.226 kb on - strand at 5080.226 kb on - strand at 5080.226 kb on - strand at 5080.226 kb on - strand at 5080.226 kb on - strand at 5080.226 kb on - strand at 5080.282 kb on + strand at 5080.283 kb on - strand at 5080.397 kb on + strand, within PS417_23455 at 5080.397 kb on + strand, within PS417_23455 at 5080.398 kb on - strand, within PS417_23455 at 5080.586 kb on + strand, within PS417_23455 at 5080.586 kb on + strand, within PS417_23455 at 5080.989 kb on - strand, within PS417_23460 at 5080.989 kb on - strand, within PS417_23460
Per-strain Table
Position Strand Gene LocusTag Fraction Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 remove 5,078,667 + +0.5 5,078,670 - +0.3 5,079,115 + PS417_23445 0.58 -0.4 5,079,115 + PS417_23445 0.58 -0.5 5,079,115 + -0.1 5,079,153 + -0.3 5,079,153 + PS417_23445 0.63 -0.3 5,079,153 + PS417_23445 0.63 -0.9 5,079,154 - PS417_23445 0.63 +0.2 5,079,155 + PS417_23445 0.63 -0.6 5,079,176 + PS417_23445 0.66 -0.6 5,079,176 + PS417_23445 0.66 +0.2 5,079,177 - PS417_23445 0.66 -0.1 5,079,303 + PS417_23445 0.82 +1.2 5,079,497 - +0.0 5,079,497 - -0.1 5,079,497 - +0.1 5,079,567 + -0.6 5,080,109 - PS417_23450 0.75 -0.8 5,080,152 + PS417_23450 0.82 -0.1 5,080,153 - PS417_23450 0.82 -0.7 5,080,182 + PS417_23450 0.86 -0.1 5,080,182 + PS417_23450 0.86 -1.2 5,080,182 + PS417_23450 0.86 -1.4 5,080,183 - PS417_23450 0.86 -0.3 5,080,183 - PS417_23450 0.86 -1.4 5,080,183 - PS417_23450 0.86 -2.0 5,080,223 + +0.6 5,080,223 + -0.0 5,080,223 + +1.3 5,080,225 + +0.3 5,080,225 + +0.6 5,080,226 - -0.1 5,080,226 - -1.2 5,080,226 - +0.6 5,080,226 - -0.4 5,080,226 - -0.8 5,080,226 - +1.2 5,080,282 + +0.4 5,080,283 - +0.7 5,080,397 + PS417_23455 0.35 -1.1 5,080,397 + PS417_23455 0.35 -0.0 5,080,398 - PS417_23455 0.35 -0.6 5,080,586 + PS417_23455 0.86 -1.9 5,080,586 + PS417_23455 0.86 +0.0 5,080,989 - PS417_23460 0.28 -0.6 5,080,989 - PS417_23460 0.28 +0.6
Or see this region's nucleotide sequence