Experiment: Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02065 and PS417_02070 overlap by 11 nucleotides PS417_02070 and PS417_02075 are separated by 29 nucleotides PS417_02075 and PS417_02080 overlap by 8 nucleotides
PS417_02065: PS417_02065 - membrane protein, at 462,463 to 464,775
_02065
PS417_02070: PS417_02070 - membrane protein, at 464,765 to 465,427
_02070
PS417_02075: PS417_02075 - thioesterase, at 465,457 to 465,882
_02075
PS417_02080: PS417_02080 - histidine ammonia-lyase, at 465,875 to 467,416
_02080
Position (kb) 464
465
466 Strain fitness (log2 ratio) -2
-1
0
1 at 463.811 kb on + strand, within PS417_02065 at 463.812 kb on - strand, within PS417_02065 at 463.835 kb on - strand, within PS417_02065 at 463.879 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 463.982 kb on - strand, within PS417_02065 at 464.083 kb on + strand, within PS417_02065 at 464.084 kb on - strand, within PS417_02065 at 464.573 kb on - strand at 464.578 kb on + strand at 464.610 kb on + strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.611 kb on - strand at 464.828 kb on - strand at 464.828 kb on - strand at 465.330 kb on - strand, within PS417_02070 at 465.476 kb on - strand at 465.596 kb on + strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.597 kb on - strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.734 kb on + strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 465.735 kb on - strand, within PS417_02075 at 466.142 kb on + strand, within PS417_02080 at 466.142 kb on + strand, within PS417_02080 at 466.317 kb on + strand, within PS417_02080
Per-strain Table
Position Strand Gene LocusTag Fraction Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 remove 463,811 + PS417_02065 0.58 +0.2 463,812 - PS417_02065 0.58 +0.1 463,835 - PS417_02065 0.59 -0.9 463,879 - PS417_02065 0.61 +0.3 463,982 - PS417_02065 0.66 -0.4 463,982 - PS417_02065 0.66 +0.7 464,083 + PS417_02065 0.70 +0.1 464,084 - PS417_02065 0.70 -0.1 464,573 - -0.1 464,578 + +1.7 464,610 + +0.1 464,611 - +0.8 464,611 - -0.2 464,611 - -0.4 464,611 - +1.3 464,828 - -0.4 464,828 - -0.4 465,330 - PS417_02070 0.85 -0.8 465,476 - +0.2 465,596 + PS417_02075 0.33 +0.5 465,597 - PS417_02075 0.33 -0.7 465,597 - PS417_02075 0.33 -0.7 465,734 + PS417_02075 0.65 +0.1 465,734 + PS417_02075 0.65 +0.7 465,734 + PS417_02075 0.65 +0.4 465,735 - PS417_02075 0.65 +1.4 465,735 - PS417_02075 0.65 -0.3 466,142 + PS417_02080 0.17 -2.1 466,142 + PS417_02080 0.17 -0.1 466,317 + PS417_02080 0.29 +1.1
Or see this region's nucleotide sequence