Strain Fitness in Pseudomonas simiae WCS417 around PS417_19805

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_19800 and PS417_19805 overlap by 8 nucleotidesPS417_19805 and PS417_19810 are separated by 16 nucleotidesPS417_19810 and PS417_19815 are separated by 131 nucleotides PS417_19800: PS417_19800 - flagellar motor switch protein FliG, at 4,298,448 to 4,299,467 _19800 PS417_19805: PS417_19805 - flagellar M-ring protein FliF, at 4,299,460 to 4,301,238 _19805 PS417_19810: PS417_19810 - flagellar hook-basal body protein FliE, at 4,301,255 to 4,301,584 _19810 PS417_19815: PS417_19815 - Fis family transcriptional regulator, at 4,301,716 to 4,303,092 _19815 Position (kb) 4299 4300 4301 4302Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4299.220 kb on + strandat 4299.221 kb on - strand, within PS417_19800at 4299.221 kb on - strand, within PS417_19800at 4299.555 kb on - strandat 4299.800 kb on + strand, within PS417_19805at 4300.702 kb on + strand, within PS417_19805at 4301.244 kb on - strandat 4301.283 kb on + strandat 4301.303 kb on - strand, within PS417_19810at 4301.303 kb on - strand, within PS417_19810at 4301.634 kb on + strandat 4301.634 kb on + strandat 4301.635 kb on - strandat 4301.645 kb on + strandat 4301.718 kb on - strandat 4301.718 kb on - strandat 4301.728 kb on + strandat 4301.729 kb on - strandat 4301.729 kb on - strandat 4301.732 kb on + strandat 4301.732 kb on + strandat 4301.733 kb on - strandat 4301.733 kb on - strandat 4301.733 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
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4,299,220 + -0.6
4,299,221 - PS417_19800 0.76 -5.5
4,299,221 - PS417_19800 0.76 -5.1
4,299,555 - -5.2
4,299,800 + PS417_19805 0.19 -4.0
4,300,702 + PS417_19805 0.70 -5.3
4,301,244 - -2.9
4,301,283 + -6.0
4,301,303 - PS417_19810 0.15 -3.2
4,301,303 - PS417_19810 0.15 -2.9
4,301,634 + -1.9
4,301,634 + -5.0
4,301,635 - -0.2
4,301,645 + -3.5
4,301,718 - -0.2
4,301,718 - -0.2
4,301,728 + -4.5
4,301,729 - -1.0
4,301,729 - -1.0
4,301,732 + -4.1
4,301,732 + -5.0
4,301,733 - +0.4
4,301,733 - +0.8
4,301,733 - +1.2

Or see this region's nucleotide sequence