Strain Fitness in Pseudomonas simiae WCS417 around PS417_00725

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00715 and PS417_00720 overlap by 4 nucleotidesPS417_00720 and PS417_00725 overlap by 4 nucleotidesPS417_00725 and PS417_00730 are separated by 81 nucleotidesPS417_00730 and PS417_00735 are separated by 147 nucleotides PS417_00715: PS417_00715 - required for octanoate transport, together with NodT, MFP, and FUSC proteins (PS417_00725, PS417_00720, PS417_00710) (from data), at 149,595 to 149,804 _00715 PS417_00720: PS417_00720 - membrane protein, at 149,801 to 150,691 _00720 PS417_00725: PS417_00725 - RND transporter, at 150,688 to 152,130 _00725 PS417_00730: PS417_00730 - NADH:ubiquinone oxidoreductase subunit N, at 152,212 to 152,844 _00730 PS417_00735: PS417_00735 - hypothetical protein, at 152,992 to 154,488 _00735 Position (kb) 150 151 152 153Strain fitness (log2 ratio) -2 -1 0 1 2at 149.709 kb on + strand, within PS417_00715at 149.891 kb on + strand, within PS417_00720at 149.891 kb on + strand, within PS417_00720at 149.891 kb on + strandat 149.891 kb on + strand, within PS417_00720at 149.891 kb on + strand, within PS417_00720at 149.892 kb on - strand, within PS417_00720at 149.892 kb on - strand, within PS417_00720at 149.892 kb on - strand, within PS417_00720at 149.892 kb on - strand, within PS417_00720at 149.894 kb on + strand, within PS417_00720at 149.894 kb on + strand, within PS417_00720at 149.894 kb on + strand, within PS417_00720at 149.894 kb on + strand, within PS417_00720at 149.894 kb on + strand, within PS417_00720at 150.670 kb on + strandat 150.757 kb on + strandat 150.772 kb on - strandat 150.830 kb on + strandat 150.830 kb on + strandat 150.831 kb on - strandat 150.831 kb on - strandat 150.868 kb on + strand, within PS417_00725at 150.868 kb on + strand, within PS417_00725at 150.868 kb on + strand, within PS417_00725at 150.869 kb on - strand, within PS417_00725at 150.961 kb on + strand, within PS417_00725at 151.375 kb on + strand, within PS417_00725at 151.376 kb on - strand, within PS417_00725at 151.376 kb on - strand, within PS417_00725at 151.376 kb on - strand, within PS417_00725at 151.865 kb on - strand, within PS417_00725at 151.865 kb on - strand, within PS417_00725at 152.000 kb on - strandat 152.141 kb on - strandat 152.224 kb on + strandat 152.224 kb on + strandat 152.261 kb on + strandat 152.278 kb on + strand, within PS417_00730at 152.278 kb on + strand, within PS417_00730at 152.278 kb on + strand, within PS417_00730at 152.279 kb on - strand, within PS417_00730at 152.389 kb on + strand, within PS417_00730at 152.497 kb on + strand, within PS417_00730at 152.512 kb on + strand, within PS417_00730at 152.513 kb on - strand, within PS417_00730at 152.698 kb on + strand, within PS417_00730at 152.836 kb on + strandat 152.837 kb on - strandat 152.899 kb on + strandat 152.899 kb on + strandat 152.997 kb on + strandat 152.997 kb on + strandat 153.009 kb on - strandat 153.009 kb on - strandat 153.064 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
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149,709 + PS417_00715 0.54 +0.8
149,891 + PS417_00720 0.10 +0.3
149,891 + PS417_00720 0.10 -0.1
149,891 + +0.2
149,891 + PS417_00720 0.10 +0.0
149,891 + PS417_00720 0.10 +1.5
149,892 - PS417_00720 0.10 -1.5
149,892 - PS417_00720 0.10 +0.4
149,892 - PS417_00720 0.10 -0.1
149,892 - PS417_00720 0.10 +0.4
149,894 + PS417_00720 0.10 +1.9
149,894 + PS417_00720 0.10 -0.7
149,894 + PS417_00720 0.10 -1.5
149,894 + PS417_00720 0.10 +0.8
149,894 + PS417_00720 0.10 -2.7
150,670 + +0.7
150,757 + +0.4
150,772 - -1.3
150,830 + -0.6
150,830 + -2.3
150,831 - -1.3
150,831 - +0.7
150,868 + PS417_00725 0.12 +0.3
150,868 + PS417_00725 0.12 -0.5
150,868 + PS417_00725 0.12 +0.4
150,869 - PS417_00725 0.13 -2.1
150,961 + PS417_00725 0.19 -1.0
151,375 + PS417_00725 0.48 -0.8
151,376 - PS417_00725 0.48 -0.8
151,376 - PS417_00725 0.48 +0.6
151,376 - PS417_00725 0.48 -0.8
151,865 - PS417_00725 0.82 +1.9
151,865 - PS417_00725 0.82 -0.6
152,000 - -0.6
152,141 - +0.1
152,224 + +0.3
152,224 + +1.2
152,261 + +0.1
152,278 + PS417_00730 0.10 +0.1
152,278 + PS417_00730 0.10 +0.4
152,278 + PS417_00730 0.10 +0.7
152,279 - PS417_00730 0.11 -2.2
152,389 + PS417_00730 0.28 +0.4
152,497 + PS417_00730 0.45 -1.0
152,512 + PS417_00730 0.47 -1.4
152,513 - PS417_00730 0.48 +0.3
152,698 + PS417_00730 0.77 -0.3
152,836 + +1.2
152,837 - -0.3
152,899 + +0.4
152,899 + +0.1
152,997 + -0.2
152,997 + +0.7
153,009 - -0.2
153,009 - -0.8
153,064 + -1.7

Or see this region's nucleotide sequence