Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4501 and PP_4502 overlap by 32 nucleotides PP_4502 and PP_4503 are separated by 164 nucleotides PP_4503 and PP_4504 are separated by 162 nucleotides PP_4504 and PP_4505 are separated by 58 nucleotides
PP_4501: PP_4501 - Probable intracellular septation protein A, at 5,115,853 to 5,116,446
_4501
PP_4502: PP_4502 - putative enzyme, at 5,116,415 to 5,116,747
_4502
PP_4503: PP_4503 - DNA-binding response regulator, at 5,116,912 to 5,117,589
_4503
PP_4504: PP_4504 - conserved exported protein of unknown function, at 5,117,752 to 5,118,168
_4504
PP_4505: PP_4505 - Sensor histidine kinase, at 5,118,227 to 5,119,567
_4505
Position (kb) 5116
5117
5118 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5116.425 kb on + strand at 5116.425 kb on + strand at 5116.426 kb on - strand at 5116.426 kb on - strand at 5116.454 kb on + strand, within PP_4502 at 5116.621 kb on + strand, within PP_4502 at 5116.685 kb on + strand, within PP_4502 at 5116.685 kb on + strand at 5116.686 kb on - strand, within PP_4502 at 5116.686 kb on - strand, within PP_4502 at 5116.686 kb on - strand, within PP_4502 at 5116.703 kb on + strand, within PP_4502 at 5116.737 kb on + strand at 5116.738 kb on - strand at 5116.738 kb on - strand at 5116.774 kb on - strand at 5116.832 kb on + strand at 5116.832 kb on + strand at 5116.832 kb on + strand at 5116.833 kb on - strand at 5116.833 kb on - strand at 5116.835 kb on - strand at 5116.835 kb on - strand at 5117.060 kb on + strand, within PP_4503 at 5117.259 kb on - strand, within PP_4503 at 5117.318 kb on - strand, within PP_4503 at 5117.473 kb on + strand, within PP_4503 at 5117.474 kb on - strand, within PP_4503 at 5117.549 kb on + strand at 5117.550 kb on - strand at 5117.550 kb on - strand at 5117.572 kb on + strand at 5117.573 kb on - strand at 5117.575 kb on + strand at 5117.575 kb on + strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.578 kb on + strand at 5117.579 kb on - strand at 5117.579 kb on - strand at 5117.587 kb on + strand at 5117.587 kb on + strand at 5117.658 kb on + strand at 5117.658 kb on + strand at 5117.659 kb on - strand at 5117.669 kb on + strand at 5117.670 kb on - strand at 5117.960 kb on - strand, within PP_4504 at 5117.960 kb on - strand, within PP_4504 at 5118.166 kb on + strand at 5118.166 kb on + strand at 5118.167 kb on - strand at 5118.167 kb on - strand at 5118.167 kb on - strand at 5118.221 kb on + strand at 5118.222 kb on - strand at 5118.393 kb on - strand, within PP_4505 at 5118.587 kb on + strand, within PP_4505
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 5,116,425 + -0.0 5,116,425 + +1.6 5,116,426 - -1.0 5,116,426 - -2.3 5,116,454 + PP_4502 0.12 +0.6 5,116,621 + PP_4502 0.62 -0.9 5,116,685 + PP_4502 0.81 -1.6 5,116,685 + +1.4 5,116,686 - PP_4502 0.81 -0.8 5,116,686 - PP_4502 0.81 +0.3 5,116,686 - PP_4502 0.81 -0.2 5,116,703 + PP_4502 0.86 -0.7 5,116,737 + -0.4 5,116,738 - -0.6 5,116,738 - -2.7 5,116,774 - -0.5 5,116,832 + +1.1 5,116,832 + +0.5 5,116,832 + +0.1 5,116,833 - +1.7 5,116,833 - -0.5 5,116,835 - +0.1 5,116,835 - -3.2 5,117,060 + PP_4503 0.22 +0.8 5,117,259 - PP_4503 0.51 -0.5 5,117,318 - PP_4503 0.60 -3.7 5,117,473 + PP_4503 0.83 +0.8 5,117,474 - PP_4503 0.83 +1.3 5,117,549 + -0.7 5,117,550 - +0.0 5,117,550 - -0.4 5,117,572 + -3.7 5,117,573 - -0.6 5,117,575 + +0.6 5,117,575 + -1.0 5,117,576 - -0.4 5,117,576 - -0.1 5,117,576 - +0.3 5,117,576 - +0.2 5,117,576 - +0.3 5,117,576 - +0.1 5,117,578 + +0.3 5,117,579 - -0.5 5,117,579 - -0.3 5,117,587 + +0.7 5,117,587 + +0.4 5,117,658 + +0.6 5,117,658 + +0.7 5,117,659 - +0.6 5,117,669 + +0.2 5,117,670 - +0.9 5,117,960 - PP_4504 0.50 -0.0 5,117,960 - PP_4504 0.50 -1.3 5,118,166 + -4.1 5,118,166 + +0.4 5,118,167 - -0.3 5,118,167 - +0.1 5,118,167 - -0.1 5,118,221 + -3.4 5,118,222 - +0.2 5,118,393 - PP_4505 0.12 +0.3 5,118,587 + PP_4505 0.27 -1.8
Or see this region's nucleotide sequence