Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3914 and PP_5646 overlap by 4 nucleotides PP_5646 and PP_3915 overlap by 4 nucleotides PP_3915 and PP_3916 overlap by 4 nucleotides PP_3916 and PP_5647 are separated by 189 nucleotides
PP_3914: PP_3914 - conserved protein of unknown function, at 4,423,113 to 4,423,496
_3914
PP_5646: PP_5646 - protein of unknown function, at 4,423,493 to 4,423,771
_5646
PP_3915: PP_3915 - conserved protein of unknown function, at 4,423,768 to 4,424,091
_3915
PP_3916: PP_3916 - conserved protein of unknown function, at 4,424,088 to 4,424,480
_3916
PP_5647: PP_5647 - conserved protein of unknown function, at 4,424,670 to 4,424,942
_5647
Position (kb) 4424
4425 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4423.183 kb on + strand, within PP_3914 at 4423.183 kb on + strand, within PP_3914 at 4423.406 kb on + strand, within PP_3914 at 4423.406 kb on + strand, within PP_3914 at 4423.406 kb on + strand, within PP_3914 at 4423.407 kb on - strand, within PP_3914 at 4423.407 kb on - strand, within PP_3914 at 4423.407 kb on - strand, within PP_3914 at 4423.407 kb on - strand, within PP_3914 at 4423.432 kb on - strand, within PP_3914 at 4423.455 kb on + strand, within PP_3914 at 4423.455 kb on + strand, within PP_3914 at 4423.455 kb on + strand, within PP_3914 at 4423.456 kb on - strand, within PP_3914 at 4423.552 kb on + strand, within PP_5646 at 4423.553 kb on - strand, within PP_5646 at 4423.553 kb on - strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.563 kb on + strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.564 kb on - strand, within PP_5646 at 4423.594 kb on + strand, within PP_5646 at 4423.594 kb on + strand, within PP_5646 at 4423.595 kb on - strand, within PP_5646 at 4423.607 kb on + strand, within PP_5646 at 4423.607 kb on + strand, within PP_5646 at 4423.607 kb on + strand, within PP_5646 at 4423.641 kb on + strand, within PP_5646 at 4423.641 kb on + strand, within PP_5646 at 4423.641 kb on + strand, within PP_5646 at 4423.642 kb on - strand, within PP_5646 at 4423.642 kb on - strand, within PP_5646 at 4423.642 kb on - strand, within PP_5646 at 4423.720 kb on + strand, within PP_5646 at 4423.760 kb on + strand at 4423.760 kb on + strand at 4423.761 kb on - strand at 4423.761 kb on - strand at 4423.942 kb on + strand, within PP_3915 at 4423.942 kb on + strand, within PP_3915 at 4423.942 kb on + strand, within PP_3915 at 4423.943 kb on - strand, within PP_3915 at 4423.981 kb on + strand, within PP_3915 at 4423.981 kb on + strand, within PP_3915 at 4423.981 kb on + strand, within PP_3915 at 4423.981 kb on + strand, within PP_3915 at 4423.982 kb on - strand, within PP_3915 at 4423.982 kb on - strand, within PP_3915 at 4423.982 kb on - strand, within PP_3915 at 4424.032 kb on + strand, within PP_3915 at 4424.062 kb on + strand at 4424.062 kb on + strand at 4424.062 kb on + strand at 4424.062 kb on + strand at 4424.063 kb on - strand at 4424.224 kb on - strand, within PP_3916 at 4424.344 kb on - strand, within PP_3916 at 4424.425 kb on + strand, within PP_3916 at 4424.426 kb on - strand, within PP_3916 at 4424.501 kb on + strand at 4424.501 kb on + strand at 4424.501 kb on + strand at 4424.502 kb on - strand at 4424.502 kb on - strand at 4424.502 kb on - strand at 4424.502 kb on - strand at 4424.502 kb on - strand at 4424.510 kb on + strand at 4424.510 kb on + strand at 4424.511 kb on - strand at 4424.511 kb on - strand at 4424.519 kb on + strand at 4424.519 kb on + strand at 4424.520 kb on - strand at 4424.660 kb on - strand at 4424.660 kb on - strand at 4424.672 kb on - strand at 4424.735 kb on + strand, within PP_5647 at 4424.735 kb on + strand, within PP_5647 at 4424.735 kb on + strand, within PP_5647 at 4424.736 kb on - strand, within PP_5647 at 4424.758 kb on + strand, within PP_5647 at 4424.759 kb on - strand, within PP_5647 at 4424.759 kb on - strand, within PP_5647 at 4424.794 kb on + strand, within PP_5647 at 4424.794 kb on + strand, within PP_5647 at 4424.794 kb on + strand, within PP_5647 at 4424.795 kb on - strand, within PP_5647 at 4424.795 kb on - strand, within PP_5647 at 4424.942 kb on + strand at 4424.942 kb on + strand at 4424.942 kb on + strand at 4424.942 kb on + strand at 4424.942 kb on + strand at 4424.943 kb on - strand at 4424.943 kb on - strand at 4424.943 kb on - strand at 4424.943 kb on - strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.956 kb on + strand at 4424.957 kb on - strand at 4424.957 kb on - strand at 4424.957 kb on - strand at 4424.957 kb on - strand at 4424.961 kb on - strand at 4424.961 kb on - strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.989 kb on + strand at 4424.990 kb on - strand at 4424.990 kb on - strand at 4424.990 kb on - strand at 4424.990 kb on - strand at 4424.990 kb on - strand at 4425.006 kb on + strand at 4425.036 kb on + strand at 4425.049 kb on + strand at 4425.049 kb on + strand at 4425.049 kb on + strand at 4425.049 kb on + strand at 4425.049 kb on + strand at 4425.050 kb on - strand at 4425.050 kb on - strand at 4425.053 kb on + strand at 4425.053 kb on + strand at 4425.053 kb on + strand at 4425.053 kb on + strand at 4425.054 kb on - strand at 4425.054 kb on - strand at 4425.188 kb on + strand at 4425.188 kb on + strand at 4425.189 kb on - strand at 4425.189 kb on - strand at 4425.201 kb on + strand at 4425.202 kb on - strand at 4425.222 kb on + strand at 4425.222 kb on + strand at 4425.222 kb on + strand at 4425.222 kb on + strand at 4425.222 kb on + strand at 4425.223 kb on - strand at 4425.223 kb on - strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.226 kb on + strand at 4425.227 kb on - strand at 4425.227 kb on - strand at 4425.227 kb on - strand at 4425.227 kb on - strand at 4425.227 kb on - strand at 4425.227 kb on - strand at 4425.229 kb on - strand at 4425.229 kb on - strand at 4425.229 kb on - strand at 4425.229 kb on - strand at 4425.299 kb on - strand at 4425.306 kb on + strand at 4425.307 kb on - strand at 4425.307 kb on - strand at 4425.373 kb on + strand at 4425.374 kb on - strand at 4425.374 kb on - strand at 4425.406 kb on + strand at 4425.406 kb on + strand at 4425.406 kb on + strand at 4425.406 kb on + strand at 4425.406 kb on + strand at 4425.418 kb on + strand at 4425.418 kb on + strand at 4425.418 kb on + strand at 4425.419 kb on - strand at 4425.419 kb on - strand at 4425.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 4,423,183 + PP_3914 0.18 -1.7 4,423,183 + PP_3914 0.18 +0.7 4,423,406 + PP_3914 0.76 +0.8 4,423,406 + PP_3914 0.76 +0.6 4,423,406 + PP_3914 0.76 +0.5 4,423,407 - PP_3914 0.77 +0.2 4,423,407 - PP_3914 0.77 +0.4 4,423,407 - PP_3914 0.77 -0.9 4,423,407 - PP_3914 0.77 -1.3 4,423,432 - PP_3914 0.83 -0.2 4,423,455 + PP_3914 0.89 -2.2 4,423,455 + PP_3914 0.89 +1.0 4,423,455 + PP_3914 0.89 +0.0 4,423,456 - PP_3914 0.89 -0.5 4,423,552 + PP_5646 0.21 +0.6 4,423,553 - PP_5646 0.22 -1.2 4,423,553 - PP_5646 0.22 +0.1 4,423,563 + PP_5646 0.25 -0.1 4,423,563 + PP_5646 0.25 +0.3 4,423,563 + PP_5646 0.25 +0.7 4,423,563 + PP_5646 0.25 +0.0 4,423,563 + PP_5646 0.25 +1.0 4,423,563 + PP_5646 0.25 -0.8 4,423,563 + PP_5646 0.25 +0.3 4,423,564 - PP_5646 0.25 +0.7 4,423,564 - PP_5646 0.25 +1.5 4,423,564 - PP_5646 0.25 -1.2 4,423,564 - PP_5646 0.25 -0.3 4,423,564 - PP_5646 0.25 +0.3 4,423,564 - PP_5646 0.25 -1.4 4,423,564 - PP_5646 0.25 -0.4 4,423,564 - PP_5646 0.25 +0.5 4,423,594 + PP_5646 0.36 +0.7 4,423,594 + PP_5646 0.36 +0.8 4,423,595 - PP_5646 0.37 -0.1 4,423,607 + PP_5646 0.41 +1.1 4,423,607 + PP_5646 0.41 +0.6 4,423,607 + PP_5646 0.41 -0.4 4,423,641 + PP_5646 0.53 +1.2 4,423,641 + PP_5646 0.53 +0.6 4,423,641 + PP_5646 0.53 +0.4 4,423,642 - PP_5646 0.53 -3.3 4,423,642 - PP_5646 0.53 -0.2 4,423,642 - PP_5646 0.53 +0.8 4,423,720 + PP_5646 0.81 -0.4 4,423,760 + -1.8 4,423,760 + -0.1 4,423,761 - -0.4 4,423,761 - +1.0 4,423,942 + PP_3915 0.54 -0.7 4,423,942 + PP_3915 0.54 -1.1 4,423,942 + PP_3915 0.54 -1.0 4,423,943 - PP_3915 0.54 -0.9 4,423,981 + PP_3915 0.66 -3.1 4,423,981 + PP_3915 0.66 -0.8 4,423,981 + PP_3915 0.66 +0.1 4,423,981 + PP_3915 0.66 -3.3 4,423,982 - PP_3915 0.66 +0.6 4,423,982 - PP_3915 0.66 -0.2 4,423,982 - PP_3915 0.66 -0.3 4,424,032 + PP_3915 0.81 -0.6 4,424,062 + -0.1 4,424,062 + -3.1 4,424,062 + -1.3 4,424,062 + +0.2 4,424,063 - +0.9 4,424,224 - PP_3916 0.35 +2.4 4,424,344 - PP_3916 0.65 +0.8 4,424,425 + PP_3916 0.86 +0.5 4,424,426 - PP_3916 0.86 -0.3 4,424,501 + +0.4 4,424,501 + +0.0 4,424,501 + +0.8 4,424,502 - +0.3 4,424,502 - +0.5 4,424,502 - +0.4 4,424,502 - +2.0 4,424,502 - -3.1 4,424,510 + -0.8 4,424,510 + +1.8 4,424,511 - -3.5 4,424,511 - +0.4 4,424,519 + +0.1 4,424,519 + +0.3 4,424,520 - -1.8 4,424,660 - -0.8 4,424,660 - -0.2 4,424,672 - -0.9 4,424,735 + PP_5647 0.24 -4.1 4,424,735 + PP_5647 0.24 +0.4 4,424,735 + PP_5647 0.24 +0.0 4,424,736 - PP_5647 0.24 -0.1 4,424,758 + PP_5647 0.32 -1.2 4,424,759 - PP_5647 0.33 +0.8 4,424,759 - PP_5647 0.33 +0.3 4,424,794 + PP_5647 0.45 -0.2 4,424,794 + PP_5647 0.45 +0.0 4,424,794 + PP_5647 0.45 -1.5 4,424,795 - PP_5647 0.46 +0.4 4,424,795 - PP_5647 0.46 -3.3 4,424,942 + -0.3 4,424,942 + -0.2 4,424,942 + -0.7 4,424,942 + -0.1 4,424,942 + -0.1 4,424,943 - +0.2 4,424,943 - +1.0 4,424,943 - +0.5 4,424,943 - -0.5 4,424,956 + +0.6 4,424,956 + +0.8 4,424,956 + +0.6 4,424,956 + +0.5 4,424,956 + +0.5 4,424,956 + -3.8 4,424,956 + -0.5 4,424,956 + -0.5 4,424,956 + +1.1 4,424,957 - -1.7 4,424,957 - -0.1 4,424,957 - +0.7 4,424,957 - +0.0 4,424,961 - +1.1 4,424,961 - +0.6 4,424,989 + -0.2 4,424,989 + -0.8 4,424,989 + +0.2 4,424,989 + -1.5 4,424,989 + +0.5 4,424,989 + +0.7 4,424,989 + -0.2 4,424,990 - +1.2 4,424,990 - +0.5 4,424,990 - +0.3 4,424,990 - +0.7 4,424,990 - +1.1 4,425,006 + +0.7 4,425,036 + -0.3 4,425,049 + +1.0 4,425,049 + -0.9 4,425,049 + -0.4 4,425,049 + +0.1 4,425,049 + -1.6 4,425,050 - -2.0 4,425,050 - +0.6 4,425,053 + -1.2 4,425,053 + -0.4 4,425,053 + +0.2 4,425,053 + -0.2 4,425,054 - +0.3 4,425,054 - +0.0 4,425,188 + +1.5 4,425,188 + +0.7 4,425,189 - -0.8 4,425,189 - -0.7 4,425,201 + +0.6 4,425,202 - +1.0 4,425,222 + -1.2 4,425,222 + -0.1 4,425,222 + +0.5 4,425,222 + +0.0 4,425,222 + -0.7 4,425,223 - -1.8 4,425,223 - -0.6 4,425,226 + +1.4 4,425,226 + +0.4 4,425,226 + +2.5 4,425,226 + -0.5 4,425,226 + +0.2 4,425,226 + -3.6 4,425,226 + +1.6 4,425,226 + +2.3 4,425,226 + +0.2 4,425,227 - -0.8 4,425,227 - -0.3 4,425,227 - +0.8 4,425,227 - -0.4 4,425,227 - +0.2 4,425,227 - +0.3 4,425,229 - -1.8 4,425,229 - -1.0 4,425,229 - -1.4 4,425,229 - +1.4 4,425,299 - +0.6 4,425,306 + -0.2 4,425,307 - -0.6 4,425,307 - +1.8 4,425,373 + +0.4 4,425,374 - -0.7 4,425,374 - +0.4 4,425,406 + -0.1 4,425,406 + -0.0 4,425,406 + -1.6 4,425,406 + +0.2 4,425,406 + -1.0 4,425,418 + +0.6 4,425,418 + -0.6 4,425,418 + -0.3 4,425,419 - -0.5 4,425,419 - -1.9 4,425,450 + +1.0
Or see this region's nucleotide sequence