Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3741 and PP_3742 are separated by 42 nucleotides PP_3742 and PP_3743 are separated by 281 nucleotides PP_3743 and PP_3744 are separated by 60 nucleotides
PP_3741: PP_3741 - transpeptidase (penicillin-binding protein 2), at 4,269,861 to 4,271,756
_3741
PP_3742: PP_3742 - Glutathione S-transferase family protein, at 4,271,799 to 4,272,425
_3742
PP_3743: PP_3743 - conserved exported protein of unknown function, at 4,272,707 to 4,273,024
_3743
PP_3744: PP_3744 - transcriptional dual regulator GlcC-Glycolate, at 4,273,085 to 4,273,876
_3744
Position (kb) 4272
4273
4274 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4271.827 kb on + strand at 4271.828 kb on - strand at 4271.930 kb on - strand, within PP_3742 at 4272.009 kb on - strand, within PP_3742 at 4272.009 kb on - strand, within PP_3742 at 4272.014 kb on - strand, within PP_3742 at 4272.100 kb on + strand, within PP_3742 at 4272.101 kb on - strand, within PP_3742 at 4272.103 kb on + strand, within PP_3742 at 4272.103 kb on + strand, within PP_3742 at 4272.104 kb on - strand, within PP_3742 at 4272.104 kb on - strand, within PP_3742 at 4272.104 kb on - strand, within PP_3742 at 4272.104 kb on - strand, within PP_3742 at 4272.116 kb on + strand, within PP_3742 at 4272.116 kb on + strand, within PP_3742 at 4272.380 kb on - strand at 4272.380 kb on - strand at 4272.380 kb on - strand at 4272.633 kb on + strand at 4272.633 kb on + strand at 4272.655 kb on + strand at 4272.656 kb on - strand at 4272.672 kb on - strand at 4272.672 kb on - strand at 4272.692 kb on - strand at 4272.717 kb on + strand at 4272.718 kb on - strand at 4272.718 kb on - strand at 4272.806 kb on - strand, within PP_3743 at 4272.822 kb on - strand, within PP_3743 at 4272.911 kb on + strand, within PP_3743 at 4272.911 kb on + strand, within PP_3743 at 4272.911 kb on + strand, within PP_3743 at 4272.912 kb on - strand, within PP_3743 at 4272.912 kb on - strand, within PP_3743 at 4272.912 kb on - strand, within PP_3743 at 4272.912 kb on - strand, within PP_3743 at 4273.073 kb on + strand at 4273.074 kb on - strand at 4273.102 kb on + strand at 4273.103 kb on - strand at 4273.163 kb on + strand at 4273.163 kb on + strand at 4273.174 kb on + strand, within PP_3744 at 4273.174 kb on + strand, within PP_3744 at 4273.174 kb on + strand, within PP_3744 at 4273.175 kb on - strand, within PP_3744 at 4273.175 kb on - strand, within PP_3744 at 4273.175 kb on - strand, within PP_3744 at 4273.282 kb on - strand, within PP_3744 at 4273.343 kb on - strand, within PP_3744 at 4273.361 kb on - strand, within PP_3744 at 4273.361 kb on - strand, within PP_3744 at 4273.479 kb on - strand, within PP_3744 at 4273.606 kb on + strand, within PP_3744 at 4273.606 kb on + strand, within PP_3744 at 4273.884 kb on + strand at 4273.885 kb on + strand at 4273.885 kb on - strand at 4273.917 kb on + strand at 4273.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 4,271,827 + +0.2 4,271,828 - -0.1 4,271,930 - PP_3742 0.21 +1.0 4,272,009 - PP_3742 0.33 -1.3 4,272,009 - PP_3742 0.33 +1.9 4,272,014 - PP_3742 0.34 -0.8 4,272,100 + PP_3742 0.48 -1.7 4,272,101 - PP_3742 0.48 -1.3 4,272,103 + PP_3742 0.48 +0.9 4,272,103 + PP_3742 0.48 -0.3 4,272,104 - PP_3742 0.49 -1.8 4,272,104 - PP_3742 0.49 -0.0 4,272,104 - PP_3742 0.49 +0.7 4,272,104 - PP_3742 0.49 -0.2 4,272,116 + PP_3742 0.51 -0.2 4,272,116 + PP_3742 0.51 -0.0 4,272,380 - +1.0 4,272,380 - +0.4 4,272,380 - -1.1 4,272,633 + +0.4 4,272,633 + +0.6 4,272,655 + +0.1 4,272,656 - +0.1 4,272,672 - -0.0 4,272,672 - -0.6 4,272,692 - +1.3 4,272,717 + +0.8 4,272,718 - +0.6 4,272,718 - +0.5 4,272,806 - PP_3743 0.31 +0.7 4,272,822 - PP_3743 0.36 +0.7 4,272,911 + PP_3743 0.64 +0.4 4,272,911 + PP_3743 0.64 +0.2 4,272,911 + PP_3743 0.64 -0.3 4,272,912 - PP_3743 0.64 -4.0 4,272,912 - PP_3743 0.64 +0.4 4,272,912 - PP_3743 0.64 +0.8 4,272,912 - PP_3743 0.64 +2.7 4,273,073 + -0.7 4,273,074 - -1.0 4,273,102 + -0.5 4,273,103 - -0.5 4,273,163 + -0.1 4,273,163 + +0.5 4,273,174 + PP_3744 0.11 +1.1 4,273,174 + PP_3744 0.11 +0.3 4,273,174 + PP_3744 0.11 +1.1 4,273,175 - PP_3744 0.11 +0.3 4,273,175 - PP_3744 0.11 -0.4 4,273,175 - PP_3744 0.11 +0.0 4,273,282 - PP_3744 0.25 +0.3 4,273,343 - PP_3744 0.33 +0.6 4,273,361 - PP_3744 0.35 +0.2 4,273,361 - PP_3744 0.35 -1.3 4,273,479 - PP_3744 0.50 +1.0 4,273,606 + PP_3744 0.66 +0.9 4,273,606 + PP_3744 0.66 +0.7 4,273,884 + -0.2 4,273,885 + -0.1 4,273,885 - -0.3 4,273,917 + -1.4 4,273,917 + -0.1
Or see this region's nucleotide sequence