Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3538 and PP_3539 are separated by 75 nucleotides PP_3539 and PP_3540 are separated by 19 nucleotides
PP_3538: PP_3538 - transcriptional regulator, at 4,010,793 to 4,011,671
_3538
PP_3539: PP_3539 - putative Transcriptional regulator, at 4,011,747 to 4,012,145
_3539
PP_3540: PP_3540 - Hydroxymethylglutaryl-CoA lyase, at 4,012,165 to 4,013,064
_3540
Position (kb)
4011
4012
4013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4010.773 kb on - strand at 4010.833 kb on - strand at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.955 kb on + strand, within PP_3538 at 4010.956 kb on - strand, within PP_3538 at 4010.956 kb on - strand, within PP_3538 at 4010.956 kb on - strand, within PP_3538 at 4010.956 kb on - strand, within PP_3538 at 4010.956 kb on - strand, within PP_3538 at 4010.959 kb on + strand, within PP_3538 at 4010.960 kb on - strand, within PP_3538 at 4010.960 kb on - strand, within PP_3538 at 4011.082 kb on - strand, within PP_3538 at 4011.082 kb on - strand, within PP_3538 at 4011.098 kb on + strand, within PP_3538 at 4011.099 kb on - strand, within PP_3538 at 4011.109 kb on + strand, within PP_3538 at 4011.109 kb on + strand, within PP_3538 at 4011.110 kb on - strand, within PP_3538 at 4011.110 kb on - strand, within PP_3538 at 4011.229 kb on - strand, within PP_3538 at 4011.366 kb on + strand, within PP_3538 at 4011.367 kb on - strand, within PP_3538 at 4011.367 kb on - strand, within PP_3538 at 4011.536 kb on + strand at 4011.536 kb on + strand, within PP_3538 at 4011.536 kb on + strand, within PP_3538 at 4011.536 kb on + strand, within PP_3538 at 4011.537 kb on - strand, within PP_3538 at 4011.537 kb on - strand, within PP_3538 at 4011.540 kb on + strand, within PP_3538 at 4011.670 kb on - strand at 4011.708 kb on - strand at 4011.762 kb on + strand at 4011.762 kb on + strand at 4011.762 kb on + strand at 4011.870 kb on + strand, within PP_3539 at 4011.870 kb on + strand, within PP_3539 at 4011.870 kb on + strand, within PP_3539 at 4012.294 kb on + strand, within PP_3540 at 4012.458 kb on - strand, within PP_3540 at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.062 kb on + strand at 4013.063 kb on - strand at 4013.100 kb on + strand at 4013.100 kb on + strand at 4013.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 4,010,773 - -1.5 4,010,833 - +0.9 4,010,955 + PP_3538 0.18 +0.2 4,010,955 + PP_3538 0.18 +1.3 4,010,955 + PP_3538 0.18 -2.1 4,010,955 + PP_3538 0.18 -0.2 4,010,955 + PP_3538 0.18 +0.8 4,010,955 + PP_3538 0.18 -0.3 4,010,955 + PP_3538 0.18 +0.1 4,010,955 + PP_3538 0.18 +1.0 4,010,955 + PP_3538 0.18 -0.9 4,010,955 + PP_3538 0.18 -0.6 4,010,955 + PP_3538 0.18 +0.2 4,010,956 - PP_3538 0.19 +1.0 4,010,956 - PP_3538 0.19 +1.1 4,010,956 - PP_3538 0.19 +0.0 4,010,956 - PP_3538 0.19 +0.3 4,010,956 - PP_3538 0.19 -0.8 4,010,959 + PP_3538 0.19 +0.6 4,010,960 - PP_3538 0.19 +0.8 4,010,960 - PP_3538 0.19 +0.9 4,011,082 - PP_3538 0.33 -2.2 4,011,082 - PP_3538 0.33 +0.0 4,011,098 + PP_3538 0.35 +0.6 4,011,099 - PP_3538 0.35 -2.2 4,011,109 + PP_3538 0.36 +0.9 4,011,109 + PP_3538 0.36 -3.4 4,011,110 - PP_3538 0.36 -1.3 4,011,110 - PP_3538 0.36 +0.6 4,011,229 - PP_3538 0.50 +0.4 4,011,366 + PP_3538 0.65 +0.2 4,011,367 - PP_3538 0.65 -0.4 4,011,367 - PP_3538 0.65 +1.4 4,011,536 + +0.2 4,011,536 + PP_3538 0.85 -0.3 4,011,536 + PP_3538 0.85 -1.6 4,011,536 + PP_3538 0.85 +0.1 4,011,537 - PP_3538 0.85 -0.4 4,011,537 - PP_3538 0.85 +2.1 4,011,540 + PP_3538 0.85 -1.6 4,011,670 - +0.5 4,011,708 - +1.6 4,011,762 + -0.9 4,011,762 + +1.6 4,011,762 + +0.4 4,011,870 + PP_3539 0.31 -0.0 4,011,870 + PP_3539 0.31 +1.0 4,011,870 + PP_3539 0.31 +0.8 4,012,294 + PP_3540 0.14 +2.2 4,012,458 - PP_3540 0.33 -0.4 4,013,062 + +0.6 4,013,062 + -0.3 4,013,062 + +0.8 4,013,062 + -0.3 4,013,063 - +1.3 4,013,100 + +0.7 4,013,100 + +0.2 4,013,101 - -0.9
Or see this region's nucleotide sequence