Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2723 and PP_2724 are separated by 194 nucleotides PP_2724 and PP_2725 are separated by 56 nucleotides PP_2725 and PP_2726 are separated by 89 nucleotides PP_2726 and PP_2727 are separated by 95 nucleotides
PP_2723: PP_2723 - Uncharacterized oxidoreductase YhdF, at 3,109,323 to 3,110,180
_2723
PP_2724: PP_2724 - conserved protein of unknown function, at 3,110,375 to 3,110,899
_2724
PP_2725: PP_2725 - Protease PfpI, at 3,110,956 to 3,111,498
_2725
PP_2726: PP_2726 - conserved membrane protein of unknown function, at 3,111,588 to 3,112,280
_2726
PP_2727: PP_2727 - putative C-factor, at 3,112,376 to 3,113,146
_2727
Position (kb)
3110
3111
3112 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3109.964 kb on - strand, within PP_2723 at 3109.996 kb on + strand, within PP_2723 at 3109.997 kb on - strand, within PP_2723 at 3110.081 kb on - strand, within PP_2723 at 3110.166 kb on - strand at 3110.166 kb on - strand at 3110.313 kb on + strand at 3110.487 kb on - strand, within PP_2724 at 3110.555 kb on + strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.690 kb on - strand, within PP_2724 at 3110.753 kb on + strand, within PP_2724 at 3110.753 kb on + strand, within PP_2724 at 3111.063 kb on + strand, within PP_2725 at 3111.102 kb on - strand, within PP_2725 at 3111.102 kb on - strand, within PP_2725 at 3111.355 kb on + strand, within PP_2725 at 3111.516 kb on - strand at 3111.526 kb on + strand at 3111.526 kb on + strand at 3111.574 kb on - strand at 3111.629 kb on + strand at 3111.629 kb on + strand at 3111.629 kb on + strand at 3111.630 kb on - strand at 3111.630 kb on - strand at 3111.790 kb on + strand, within PP_2726 at 3111.797 kb on - strand, within PP_2726 at 3111.871 kb on - strand, within PP_2726 at 3111.922 kb on - strand, within PP_2726 at 3111.928 kb on + strand, within PP_2726 at 3111.929 kb on - strand, within PP_2726 at 3112.065 kb on + strand, within PP_2726 at 3112.232 kb on + strand at 3112.248 kb on + strand at 3112.248 kb on + strand at 3112.249 kb on - strand at 3112.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 3,109,964 - PP_2723 0.75 -0.2 3,109,996 + PP_2723 0.78 +0.4 3,109,997 - PP_2723 0.79 -0.3 3,110,081 - PP_2723 0.88 +0.5 3,110,166 - -0.0 3,110,166 - +0.2 3,110,313 + -3.9 3,110,487 - PP_2724 0.21 +0.2 3,110,555 + PP_2724 0.34 +1.3 3,110,556 - PP_2724 0.34 -1.2 3,110,556 - PP_2724 0.34 +0.3 3,110,556 - PP_2724 0.34 +1.3 3,110,690 - PP_2724 0.60 +0.0 3,110,753 + PP_2724 0.72 +1.1 3,110,753 + PP_2724 0.72 -0.4 3,111,063 + PP_2725 0.20 -0.3 3,111,102 - PP_2725 0.27 +0.8 3,111,102 - PP_2725 0.27 +1.0 3,111,355 + PP_2725 0.73 +2.2 3,111,516 - -0.7 3,111,526 + -0.1 3,111,526 + -0.8 3,111,574 - +1.2 3,111,629 + -0.3 3,111,629 + -0.8 3,111,629 + -0.3 3,111,630 - +0.8 3,111,630 - +1.2 3,111,790 + PP_2726 0.29 -0.8 3,111,797 - PP_2726 0.30 +0.2 3,111,871 - PP_2726 0.41 -1.3 3,111,922 - PP_2726 0.48 +1.8 3,111,928 + PP_2726 0.49 +0.7 3,111,929 - PP_2726 0.49 -0.6 3,112,065 + PP_2726 0.69 -0.4 3,112,232 + +0.2 3,112,248 + -0.4 3,112,248 + -0.4 3,112,249 - -1.5 3,112,312 + -2.9
Or see this region's nucleotide sequence