Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1419 and PP_1420 are separated by 200 nucleotides PP_1420 and PP_1421 overlap by 20 nucleotides
PP_1419: PP_1419 - tricarboxylate-specific outer membrane porin, at 1,618,945 to 1,620,234
_1419
PP_1420: PP_1420 - Transcriptional regulatory protein tctD, at 1,620,435 to 1,621,106
_1420
PP_1421: PP_1421 - putative Sensor histidine kinase TctE, at 1,621,087 to 1,622,478
_1421
Position (kb)
1620
1621
1622 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1619.501 kb on + strand, within PP_1419 at 1619.506 kb on - strand, within PP_1419 at 1619.506 kb on - strand, within PP_1419 at 1619.528 kb on + strand, within PP_1419 at 1619.528 kb on + strand, within PP_1419 at 1619.558 kb on + strand, within PP_1419 at 1619.559 kb on - strand, within PP_1419 at 1619.855 kb on + strand, within PP_1419 at 1620.077 kb on + strand, within PP_1419 at 1620.078 kb on - strand, within PP_1419 at 1620.078 kb on - strand, within PP_1419 at 1620.190 kb on - strand at 1620.261 kb on - strand at 1620.318 kb on + strand at 1620.318 kb on + strand at 1620.318 kb on + strand at 1620.318 kb on + strand at 1620.394 kb on + strand at 1620.445 kb on + strand at 1620.446 kb on - strand at 1620.446 kb on - strand at 1620.514 kb on + strand, within PP_1420 at 1620.514 kb on + strand, within PP_1420 at 1620.514 kb on + strand, within PP_1420 at 1620.515 kb on - strand, within PP_1420 at 1620.515 kb on - strand, within PP_1420 at 1620.515 kb on - strand, within PP_1420 at 1620.567 kb on + strand, within PP_1420 at 1620.567 kb on + strand, within PP_1420 at 1620.567 kb on + strand, within PP_1420 at 1620.568 kb on - strand, within PP_1420 at 1620.619 kb on + strand, within PP_1420 at 1620.619 kb on + strand, within PP_1420 at 1620.620 kb on - strand, within PP_1420 at 1620.662 kb on + strand, within PP_1420 at 1620.663 kb on - strand, within PP_1420 at 1620.663 kb on - strand, within PP_1420 at 1620.782 kb on - strand, within PP_1420 at 1620.782 kb on - strand, within PP_1420 at 1620.782 kb on - strand, within PP_1420 at 1620.834 kb on + strand, within PP_1420 at 1620.834 kb on + strand, within PP_1420 at 1620.834 kb on + strand, within PP_1420 at 1620.835 kb on - strand, within PP_1420 at 1621.011 kb on + strand, within PP_1420 at 1621.137 kb on + strand at 1621.182 kb on + strand at 1621.183 kb on - strand at 1621.223 kb on - strand at 1621.223 kb on - strand at 1621.306 kb on + strand, within PP_1421 at 1621.307 kb on - strand, within PP_1421 at 1621.307 kb on - strand, within PP_1421 at 1621.330 kb on + strand, within PP_1421 at 1621.331 kb on - strand, within PP_1421 at 1621.331 kb on - strand, within PP_1421 at 1621.331 kb on - strand, within PP_1421 at 1621.351 kb on + strand, within PP_1421 at 1621.351 kb on + strand, within PP_1421 at 1621.352 kb on - strand, within PP_1421 at 1621.355 kb on - strand, within PP_1421 at 1621.463 kb on - strand, within PP_1421 at 1621.463 kb on - strand, within PP_1421 at 1621.474 kb on + strand, within PP_1421 at 1621.474 kb on + strand, within PP_1421 at 1621.474 kb on + strand, within PP_1421 at 1621.474 kb on + strand, within PP_1421 at 1621.475 kb on - strand, within PP_1421
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 1,619,501 + PP_1419 0.43 -3.1 1,619,506 - PP_1419 0.43 -2.1 1,619,506 - PP_1419 0.43 -1.0 1,619,528 + PP_1419 0.45 -2.2 1,619,528 + PP_1419 0.45 -1.2 1,619,558 + PP_1419 0.48 -0.5 1,619,559 - PP_1419 0.48 -0.9 1,619,855 + PP_1419 0.71 -4.0 1,620,077 + PP_1419 0.88 -2.1 1,620,078 - PP_1419 0.88 -0.6 1,620,078 - PP_1419 0.88 -1.7 1,620,190 - -1.1 1,620,261 - +1.8 1,620,318 + -2.2 1,620,318 + -1.5 1,620,318 + -1.4 1,620,318 + -0.2 1,620,394 + -0.0 1,620,445 + -3.0 1,620,446 - -2.8 1,620,446 - -2.6 1,620,514 + PP_1420 0.12 -4.2 1,620,514 + PP_1420 0.12 -3.1 1,620,514 + PP_1420 0.12 -4.2 1,620,515 - PP_1420 0.12 -4.3 1,620,515 - PP_1420 0.12 -1.6 1,620,515 - PP_1420 0.12 -2.4 1,620,567 + PP_1420 0.20 -4.1 1,620,567 + PP_1420 0.20 -4.6 1,620,567 + PP_1420 0.20 -2.9 1,620,568 - PP_1420 0.20 -4.3 1,620,619 + PP_1420 0.27 -4.5 1,620,619 + PP_1420 0.27 -2.4 1,620,620 - PP_1420 0.28 -2.4 1,620,662 + PP_1420 0.34 -2.1 1,620,663 - PP_1420 0.34 -2.9 1,620,663 - PP_1420 0.34 -1.5 1,620,782 - PP_1420 0.52 -2.8 1,620,782 - PP_1420 0.52 -3.2 1,620,782 - PP_1420 0.52 -3.3 1,620,834 + PP_1420 0.59 -4.6 1,620,834 + PP_1420 0.59 -2.4 1,620,834 + PP_1420 0.59 -4.8 1,620,835 - PP_1420 0.60 -4.0 1,621,011 + PP_1420 0.86 -3.9 1,621,137 + -3.4 1,621,182 + -3.8 1,621,183 - -3.6 1,621,223 - -3.8 1,621,223 - -4.6 1,621,306 + PP_1421 0.16 -4.3 1,621,307 - PP_1421 0.16 -4.6 1,621,307 - PP_1421 0.16 -2.7 1,621,330 + PP_1421 0.17 -2.8 1,621,331 - PP_1421 0.18 -4.5 1,621,331 - PP_1421 0.18 -4.6 1,621,331 - PP_1421 0.18 -4.6 1,621,351 + PP_1421 0.19 -4.2 1,621,351 + PP_1421 0.19 -4.8 1,621,352 - PP_1421 0.19 -2.0 1,621,355 - PP_1421 0.19 -3.3 1,621,463 - PP_1421 0.27 -3.6 1,621,463 - PP_1421 0.27 -2.8 1,621,474 + PP_1421 0.28 -2.0 1,621,474 + PP_1421 0.28 -1.4 1,621,474 + PP_1421 0.28 -4.4 1,621,474 + PP_1421 0.28 -4.1 1,621,475 - PP_1421 0.28 -4.1
Or see this region's nucleotide sequence