Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1365 and PP_1366 are separated by 73 nucleotides PP_1366 and PP_1367 are separated by 283 nucleotides PP_1367 and PP_5468 are separated by 92 nucleotides PP_5468 and PP_1368 overlap by 95 nucleotides
PP_1365: PP_1365 - exodeoxyribonuclease I, at 1,553,929 to 1,555,362
_1365
PP_1366: PP_1366 - Transcriptional regulator MvaT, P16 subunit, at 1,555,436 to 1,555,813
_1366
PP_1367: PP_1367 - formyltetrahydrofolate hydrolase, at 1,556,097 to 1,556,948
_1367
PP_5468: PP_5468 - conserved protein of unknown function, at 1,557,041 to 1,557,274
_5468
PP_1368: PP_1368 - putative membrane protein containing a glycosyltransferase domain, at 1,557,180 to 1,558,172
_1368
Position (kb)
1556
1557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1555.349 kb on - strand at 1555.349 kb on - strand at 1555.370 kb on + strand at 1555.371 kb on - strand at 1555.425 kb on + strand at 1555.557 kb on + strand at 1555.592 kb on - strand, within PP_1366 at 1555.592 kb on - strand, within PP_1366 at 1555.854 kb on + strand at 1555.855 kb on - strand at 1555.857 kb on + strand at 1555.857 kb on + strand at 1555.858 kb on - strand at 1555.868 kb on + strand at 1555.869 kb on - strand at 1555.885 kb on - strand at 1555.885 kb on - strand at 1555.899 kb on - strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.012 kb on - strand at 1556.012 kb on - strand at 1556.034 kb on + strand at 1556.064 kb on + strand at 1556.064 kb on + strand at 1556.064 kb on + strand at 1556.106 kb on + strand at 1556.106 kb on + strand at 1556.107 kb on - strand at 1556.137 kb on + strand at 1556.138 kb on - strand at 1556.138 kb on - strand at 1556.138 kb on - strand at 1556.175 kb on + strand at 1556.175 kb on + strand at 1556.175 kb on + strand at 1556.176 kb on - strand at 1556.176 kb on - strand at 1556.176 kb on - strand at 1556.365 kb on + strand, within PP_1367 at 1556.366 kb on - strand, within PP_1367 at 1556.366 kb on - strand, within PP_1367 at 1556.516 kb on - strand, within PP_1367 at 1556.516 kb on - strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.608 kb on - strand, within PP_1367 at 1556.643 kb on + strand, within PP_1367 at 1556.643 kb on + strand, within PP_1367 at 1556.644 kb on - strand, within PP_1367 at 1556.706 kb on + strand, within PP_1367 at 1556.707 kb on - strand, within PP_1367 at 1556.757 kb on + strand, within PP_1367 at 1556.758 kb on - strand, within PP_1367 at 1556.758 kb on - strand, within PP_1367 at 1556.924 kb on + strand at 1556.925 kb on - strand at 1556.925 kb on - strand at 1557.259 kb on + strand at 1557.265 kb on + strand at 1557.265 kb on + strand at 1557.265 kb on + strand at 1557.266 kb on - strand at 1557.268 kb on + strand at 1557.269 kb on - strand at 1557.269 kb on - strand at 1557.273 kb on - strand at 1557.285 kb on + strand, within PP_1368 at 1557.444 kb on + strand, within PP_1368 at 1557.469 kb on + strand, within PP_1368 at 1557.469 kb on + strand, within PP_1368 at 1557.470 kb on - strand, within PP_1368 at 1557.470 kb on - strand, within PP_1368 at 1557.470 kb on - strand, within PP_1368 at 1557.482 kb on - strand, within PP_1368 at 1557.711 kb on + strand, within PP_1368
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 1,555,349 - +1.1 1,555,349 - +1.7 1,555,370 + +1.4 1,555,371 - +1.0 1,555,425 + -0.9 1,555,557 + +1.5 1,555,592 - PP_1366 0.41 -0.1 1,555,592 - PP_1366 0.41 +0.4 1,555,854 + -1.3 1,555,855 - +0.5 1,555,857 + +0.7 1,555,857 + +1.4 1,555,858 - +1.0 1,555,868 + +1.0 1,555,869 - -1.2 1,555,885 - -0.1 1,555,885 - -0.3 1,555,899 - -1.1 1,556,011 + +0.6 1,556,011 + +1.0 1,556,011 + +1.0 1,556,011 + -0.6 1,556,012 - +0.2 1,556,012 - +0.8 1,556,034 + +1.1 1,556,064 + -1.9 1,556,064 + +2.0 1,556,064 + +1.4 1,556,106 + +1.2 1,556,106 + -1.1 1,556,107 - +0.2 1,556,137 + +1.6 1,556,138 - +0.1 1,556,138 - +0.3 1,556,138 - +1.6 1,556,175 + -1.9 1,556,175 + -0.9 1,556,175 + -0.3 1,556,176 - -2.5 1,556,176 - +0.3 1,556,176 - +1.7 1,556,365 + PP_1367 0.31 -2.8 1,556,366 - PP_1367 0.32 +1.1 1,556,366 - PP_1367 0.32 +1.0 1,556,516 - PP_1367 0.49 +1.2 1,556,516 - PP_1367 0.49 +2.1 1,556,607 + PP_1367 0.60 +2.0 1,556,607 + PP_1367 0.60 +0.1 1,556,607 + PP_1367 0.60 -2.6 1,556,607 + PP_1367 0.60 -0.2 1,556,608 - PP_1367 0.60 +1.2 1,556,643 + PP_1367 0.64 +0.4 1,556,643 + PP_1367 0.64 +1.1 1,556,644 - PP_1367 0.64 -0.2 1,556,706 + PP_1367 0.71 +2.2 1,556,707 - PP_1367 0.72 +0.1 1,556,757 + PP_1367 0.77 -1.2 1,556,758 - PP_1367 0.78 -1.5 1,556,758 - PP_1367 0.78 +0.6 1,556,924 + +0.5 1,556,925 - -0.5 1,556,925 - +0.4 1,557,259 + -0.1 1,557,265 + +0.6 1,557,265 + +0.2 1,557,265 + +1.1 1,557,266 - +0.6 1,557,268 + +0.0 1,557,269 - +0.4 1,557,269 - +0.5 1,557,273 - -0.2 1,557,285 + PP_1368 0.11 +1.4 1,557,444 + PP_1368 0.27 +0.5 1,557,469 + PP_1368 0.29 +0.3 1,557,469 + PP_1368 0.29 +0.7 1,557,470 - PP_1368 0.29 +0.1 1,557,470 - PP_1368 0.29 -0.1 1,557,470 - PP_1368 0.29 -1.5 1,557,482 - PP_1368 0.30 +0.2 1,557,711 + PP_1368 0.53 -0.1
Or see this region's nucleotide sequence