Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1021 and PP_1022 are separated by 211 nucleotides PP_1022 and PP_1023 overlap by 29 nucleotides PP_1023 and PP_1024 overlap by 10 nucleotides
PP_1021: PP_1021 - DNA-binding transcriptional regulator, at 1,164,541 to 1,165,404
_1021
PP_1022: PP_1022 - glucose 6-phosphate-1-dehydrogenase, at 1,165,616 to 1,167,085
_1022
PP_1023: PP_1023 - 6-phosphogluconolactonase, at 1,167,057 to 1,167,785
_1023
PP_1024: PP_1024 - KHG/KDPG aldolase, at 1,167,776 to 1,168,483
_1024
Position (kb)
1165
1166
1167
1168 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1164.655 kb on + strand, within PP_1021 at 1164.656 kb on - strand, within PP_1021 at 1164.656 kb on - strand, within PP_1021 at 1164.701 kb on + strand, within PP_1021 at 1164.701 kb on + strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1165.148 kb on - strand, within PP_1021 at 1165.163 kb on + strand, within PP_1021 at 1165.163 kb on + strand, within PP_1021 at 1165.479 kb on - strand at 1165.503 kb on + strand at 1165.529 kb on + strand at 1165.552 kb on + strand at 1165.834 kb on + strand, within PP_1022 at 1165.834 kb on + strand, within PP_1022 at 1165.877 kb on + strand, within PP_1022 at 1165.910 kb on + strand, within PP_1022 at 1165.958 kb on + strand, within PP_1022 at 1165.958 kb on + strand, within PP_1022 at 1166.141 kb on + strand, within PP_1022 at 1166.242 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.582 kb on - strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.716 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.871 kb on - strand, within PP_1022 at 1166.981 kb on - strand at 1166.981 kb on - strand at 1166.981 kb on - strand at 1167.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 1,164,655 + PP_1021 0.13 +0.0 1,164,656 - PP_1021 0.13 +0.3 1,164,656 - PP_1021 0.13 -0.4 1,164,701 + PP_1021 0.19 +0.9 1,164,701 + PP_1021 0.19 -0.7 1,164,702 - PP_1021 0.19 +0.4 1,164,702 - PP_1021 0.19 -1.0 1,164,702 - PP_1021 0.19 +0.9 1,165,148 - PP_1021 0.70 +0.6 1,165,163 + PP_1021 0.72 +0.6 1,165,163 + PP_1021 0.72 -2.7 1,165,479 - +0.6 1,165,503 + -0.3 1,165,529 + +0.8 1,165,552 + -0.8 1,165,834 + PP_1022 0.15 +0.9 1,165,834 + PP_1022 0.15 -0.7 1,165,877 + PP_1022 0.18 -2.7 1,165,910 + PP_1022 0.20 -2.7 1,165,958 + PP_1022 0.23 -1.2 1,165,958 + PP_1022 0.23 -0.5 1,166,141 + PP_1022 0.36 +1.3 1,166,242 + PP_1022 0.43 +0.8 1,166,471 + PP_1022 0.58 -2.4 1,166,471 + PP_1022 0.58 +0.9 1,166,582 - PP_1022 0.66 +1.6 1,166,681 + PP_1022 0.72 +0.6 1,166,681 + PP_1022 0.72 -1.7 1,166,681 + PP_1022 0.72 +1.0 1,166,681 + PP_1022 0.72 +1.5 1,166,681 + PP_1022 0.72 +1.6 1,166,716 + PP_1022 0.75 +3.1 1,166,870 + PP_1022 0.85 +1.4 1,166,870 + PP_1022 0.85 +2.2 1,166,871 - PP_1022 0.85 +0.0 1,166,981 - +1.6 1,166,981 - +1.6 1,166,981 - -0.4 1,167,079 + -1.2
Or see this region's nucleotide sequence