Experiment: P2 dilution 10-3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnV and metU are separated by 47 nucleotides metU and glnW are separated by 15 nucleotides glnW and glnU are separated by 34 nucleotides glnU and leuW are separated by 23 nucleotides leuW and metT are separated by 9 nucleotides metT and asnB are separated by 379 nucleotides asnB and nagD are separated by 396 nucleotides
b0665: glnV - tRNA-Gln (NCBI), at 695,765 to 695,839
glnV
b0666: metU - tRNA-Met (NCBI), at 695,887 to 695,963
metU
b0668: glnW - tRNA-Gln (NCBI), at 695,979 to 696,053
glnW
b0670: glnU - tRNA-Gln (NCBI), at 696,088 to 696,162
glnU
b0672: leuW - tRNA-Leu (NCBI), at 696,186 to 696,270
leuW
b0673: metT - tRNA-Met (NCBI), at 696,280 to 696,356
metT
b0674: asnB - asparagine synthetase B (NCBI), at 696,736 to 698,400
asnB
b0675: nagD - UMP phosphatase (NCBI), at 698,797 to 699,549
nagD
Position (kb) 696
697
698
699 Strain fitness (log2 ratio) -1
0
1
2 at 695.779 kb on - strand, within glnV at 695.885 kb on - strand at 695.885 kb on - strand at 695.919 kb on - strand, within metU at 696.470 kb on + strand at 696.513 kb on - strand at 696.513 kb on - strand at 696.625 kb on + strand at 696.625 kb on + strand at 696.630 kb on - strand at 696.639 kb on - strand at 696.639 kb on - strand at 696.747 kb on - strand at 696.753 kb on + strand at 696.753 kb on + strand at 696.854 kb on + strand at 696.902 kb on + strand at 697.076 kb on + strand, within asnB at 697.157 kb on - strand, within asnB at 697.205 kb on + strand, within asnB at 697.246 kb on + strand, within asnB at 697.254 kb on - strand, within asnB at 697.260 kb on - strand, within asnB at 697.278 kb on + strand, within asnB at 697.349 kb on - strand, within asnB at 697.353 kb on - strand, within asnB at 697.393 kb on - strand, within asnB at 697.393 kb on - strand, within asnB at 697.448 kb on + strand, within asnB at 697.488 kb on - strand, within asnB at 697.578 kb on + strand, within asnB at 697.602 kb on - strand, within asnB at 697.611 kb on + strand, within asnB at 697.846 kb on + strand, within asnB at 697.928 kb on + strand, within asnB at 697.933 kb on + strand, within asnB at 697.955 kb on + strand, within asnB at 697.955 kb on + strand, within asnB at 697.956 kb on + strand, within asnB at 697.956 kb on - strand, within asnB at 697.963 kb on - strand, within asnB at 698.039 kb on + strand, within asnB at 698.044 kb on - strand, within asnB at 698.044 kb on - strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.318 kb on - strand at 698.318 kb on - strand at 698.352 kb on + strand at 698.472 kb on - strand at 698.472 kb on - strand at 698.472 kb on - strand at 698.720 kb on + strand at 698.737 kb on + strand at 698.745 kb on - strand at 698.763 kb on - strand at 698.857 kb on + strand at 698.920 kb on - strand, within nagD at 698.950 kb on + strand, within nagD at 698.950 kb on + strand, within nagD at 698.951 kb on - strand, within nagD at 699.008 kb on + strand, within nagD at 699.024 kb on + strand, within nagD at 699.028 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.285 kb on - strand, within nagD at 699.291 kb on + strand, within nagD at 699.349 kb on - strand, within nagD at 699.349 kb on - strand, within nagD at 699.361 kb on + strand, within nagD
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 remove 695,779 - glnV b0665 0.19 +0.1 695,885 - -0.1 695,885 - -0.2 695,919 - metU b0666 0.42 -0.8 696,470 + +1.2 696,513 - -0.1 696,513 - -0.4 696,625 + +0.9 696,625 + -1.1 696,630 - +0.6 696,639 - -1.0 696,639 - -0.7 696,747 - -0.5 696,753 + +1.4 696,753 + +0.2 696,854 + +0.1 696,902 + +0.1 697,076 + asnB b0674 0.20 +0.6 697,157 - asnB b0674 0.25 +0.5 697,205 + asnB b0674 0.28 -0.0 697,246 + asnB b0674 0.31 -0.7 697,254 - asnB b0674 0.31 +0.2 697,260 - asnB b0674 0.31 -0.9 697,278 + asnB b0674 0.33 +0.1 697,349 - asnB b0674 0.37 -0.3 697,353 - asnB b0674 0.37 +0.4 697,393 - asnB b0674 0.39 +0.2 697,393 - asnB b0674 0.39 +0.7 697,448 + asnB b0674 0.43 +0.3 697,488 - asnB b0674 0.45 +0.8 697,578 + asnB b0674 0.51 +2.1 697,602 - asnB b0674 0.52 +0.9 697,611 + asnB b0674 0.53 +0.0 697,846 + asnB b0674 0.67 -0.8 697,928 + asnB b0674 0.72 -0.7 697,933 + asnB b0674 0.72 -0.2 697,955 + asnB b0674 0.73 -0.8 697,955 + asnB b0674 0.73 +0.6 697,956 + asnB b0674 0.73 -0.2 697,956 - asnB b0674 0.73 -0.5 697,963 - asnB b0674 0.74 -0.2 698,039 + asnB b0674 0.78 +1.5 698,044 - asnB b0674 0.79 +0.0 698,044 - asnB b0674 0.79 -0.3 698,151 + asnB b0674 0.85 +0.1 698,151 + asnB b0674 0.85 +0.1 698,151 + asnB b0674 0.85 +0.1 698,151 + asnB b0674 0.85 -0.1 698,318 - -0.6 698,318 - -0.7 698,352 + +0.1 698,472 - -0.8 698,472 - +0.4 698,472 - +0.9 698,720 + -0.6 698,737 + -0.8 698,745 - +1.0 698,763 - -0.3 698,857 + +0.2 698,920 - nagD b0675 0.16 -0.1 698,950 + nagD b0675 0.20 -1.1 698,950 + nagD b0675 0.20 +0.1 698,951 - nagD b0675 0.20 +0.7 699,008 + nagD b0675 0.28 +0.2 699,024 + nagD b0675 0.30 +0.4 699,028 - nagD b0675 0.31 -0.5 699,263 - nagD b0675 0.62 +0.0 699,263 - nagD b0675 0.62 +0.9 699,285 - nagD b0675 0.65 +0.2 699,291 + nagD b0675 0.66 +0.7 699,349 - nagD b0675 0.73 -0.2 699,349 - nagD b0675 0.73 -0.4 699,361 + nagD b0675 0.75 -1.2
Or see this region's nucleotide sequence