Experiment: Growth at pH8 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4225 and PP_4226 are separated by 240 nucleotides PP_4226 and PP_5664 are separated by 11 nucleotides PP_5664 and PP_4227 overlap by 51 nucleotides PP_4227 and PP_4228 are separated by 58 nucleotides
PP_4225: PP_4225 - two component system DNA-binding transcriptional activator QseB, at 4,797,979 to 4,798,662
_4225
PP_4226: PP_4226 - conserved protein of unknown function, at 4,798,903 to 4,799,388
_4226
PP_5664: PP_5664 - conserved protein of unknown function, at 4,799,400 to 4,800,017
_5664
PP_4227: PP_4227 - conserved protein of unknown function, at 4,799,967 to 4,800,437
_4227
PP_4228: PP_4228 - conserved protein of unknown function, at 4,800,496 to 4,801,113
_4228
Position (kb) 4799
4800
4801 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4798.691 kb on + strand at 4798.691 kb on + strand at 4798.711 kb on - strand at 4798.721 kb on + strand at 4798.721 kb on + strand at 4798.721 kb on + strand at 4798.845 kb on + strand at 4799.014 kb on - strand, within PP_4226 at 4799.083 kb on + strand, within PP_4226 at 4799.084 kb on - strand, within PP_4226 at 4799.084 kb on - strand, within PP_4226 at 4799.084 kb on - strand, within PP_4226 at 4799.115 kb on - strand, within PP_4226 at 4799.425 kb on + strand at 4799.426 kb on - strand at 4799.426 kb on - strand at 4799.471 kb on + strand, within PP_5664 at 4799.471 kb on + strand, within PP_5664 at 4799.472 kb on - strand, within PP_5664 at 4799.472 kb on - strand, within PP_5664 at 4799.476 kb on + strand, within PP_5664 at 4799.477 kb on - strand, within PP_5664 at 4799.477 kb on - strand, within PP_5664 at 4799.517 kb on + strand, within PP_5664 at 4799.557 kb on + strand, within PP_5664 at 4799.636 kb on + strand, within PP_5664 at 4799.636 kb on + strand, within PP_5664 at 4799.641 kb on - strand, within PP_5664 at 4799.677 kb on - strand, within PP_5664 at 4799.797 kb on + strand, within PP_5664 at 4799.884 kb on - strand, within PP_5664 at 4799.962 kb on + strand at 4799.963 kb on - strand at 4799.963 kb on - strand at 4799.968 kb on + strand at 4799.968 kb on + strand at 4800.024 kb on + strand, within PP_4227 at 4800.025 kb on - strand, within PP_4227 at 4800.392 kb on + strand at 4800.468 kb on + strand at 4800.506 kb on + strand at 4800.528 kb on + strand at 4800.530 kb on + strand at 4800.530 kb on + strand at 4800.531 kb on - strand at 4800.580 kb on + strand, within PP_4228 at 4800.605 kb on + strand, within PP_4228 at 4800.654 kb on - strand, within PP_4228 at 4800.680 kb on - strand, within PP_4228 at 4800.829 kb on + strand, within PP_4228 at 4800.979 kb on + strand, within PP_4228 at 4800.979 kb on + strand, within PP_4228 at 4800.979 kb on + strand, within PP_4228
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) Trisodium citrate remove 4,798,691 + -1.4 4,798,691 + +0.4 4,798,711 - -0.8 4,798,721 + -2.1 4,798,721 + -1.1 4,798,721 + +0.7 4,798,845 + +0.6 4,799,014 - PP_4226 0.23 -3.6 4,799,083 + PP_4226 0.37 -0.6 4,799,084 - PP_4226 0.37 -1.1 4,799,084 - PP_4226 0.37 -3.6 4,799,084 - PP_4226 0.37 +0.7 4,799,115 - PP_4226 0.44 +0.0 4,799,425 + +0.3 4,799,426 - -0.6 4,799,426 - -1.8 4,799,471 + PP_5664 0.11 -1.2 4,799,471 + PP_5664 0.11 -1.9 4,799,472 - PP_5664 0.12 -0.6 4,799,472 - PP_5664 0.12 -0.0 4,799,476 + PP_5664 0.12 -1.1 4,799,477 - PP_5664 0.12 +0.1 4,799,477 - PP_5664 0.12 -1.4 4,799,517 + PP_5664 0.19 -0.3 4,799,557 + PP_5664 0.25 -0.5 4,799,636 + PP_5664 0.38 +0.8 4,799,636 + PP_5664 0.38 +1.4 4,799,641 - PP_5664 0.39 +0.2 4,799,677 - PP_5664 0.45 -2.9 4,799,797 + PP_5664 0.64 -0.1 4,799,884 - PP_5664 0.78 -1.6 4,799,962 + -0.1 4,799,963 - +0.2 4,799,963 - +0.8 4,799,968 + +1.1 4,799,968 + +0.7 4,800,024 + PP_4227 0.12 -3.2 4,800,025 - PP_4227 0.12 -0.9 4,800,392 + -0.5 4,800,468 + -0.0 4,800,506 + +0.6 4,800,528 + -0.7 4,800,530 + -1.5 4,800,530 + -2.1 4,800,531 - +1.7 4,800,580 + PP_4228 0.14 -0.2 4,800,605 + PP_4228 0.18 -1.0 4,800,654 - PP_4228 0.26 +0.6 4,800,680 - PP_4228 0.30 -1.1 4,800,829 + PP_4228 0.54 -3.2 4,800,979 + PP_4228 0.78 +0.1 4,800,979 + PP_4228 0.78 +1.2 4,800,979 + PP_4228 0.78 +0.4
Or see this region's nucleotide sequence