Strain Fitness in Pseudomonas putida KT2440 around PP_5520

Experiment: Growth at pH7 and (C) Trisodium citrate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2352 and PP_5520 are separated by 75 nucleotidesPP_5520 and PP_t41 are separated by 259 nucleotidesPP_t41 and PP_2354 are separated by 117 nucleotides PP_2352: PP_2352 - conserved protein of unknown function, at 2,683,437 to 2,684,564 _2352 PP_5520: PP_5520 - conserved protein of unknown function, at 2,684,640 to 2,684,882 _5520 PP_t41: PP_t41 - tRNA-Gln, at 2,685,142 to 2,685,216 _t41 PP_2354: PP_2354 - Sensory box histidine kinase/response regulator, at 2,685,334 to 2,687,721 _2354 Position (kb) 2684 2685Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2683.651 kb on + strand, within PP_2352at 2683.651 kb on + strand, within PP_2352at 2683.672 kb on + strand, within PP_2352at 2683.672 kb on + strand, within PP_2352at 2683.672 kb on + strand, within PP_2352at 2683.776 kb on + strand, within PP_2352at 2683.840 kb on + strand, within PP_2352at 2683.840 kb on + strand, within PP_2352at 2683.840 kb on + strand, within PP_2352at 2683.840 kb on + strand, within PP_2352at 2683.840 kb on + strand, within PP_2352at 2683.841 kb on - strand, within PP_2352at 2683.841 kb on - strand, within PP_2352at 2683.841 kb on - strand, within PP_2352at 2683.841 kb on - strand, within PP_2352at 2683.841 kb on - strand, within PP_2352at 2683.841 kb on - strandat 2683.841 kb on - strand, within PP_2352at 2683.853 kb on + strand, within PP_2352at 2684.007 kb on - strand, within PP_2352at 2684.023 kb on + strand, within PP_2352at 2684.024 kb on - strand, within PP_2352at 2684.024 kb on - strand, within PP_2352at 2684.080 kb on - strand, within PP_2352at 2684.080 kb on - strand, within PP_2352at 2684.299 kb on + strand, within PP_2352at 2684.300 kb on - strand, within PP_2352at 2684.306 kb on - strand, within PP_2352at 2684.571 kb on + strandat 2684.571 kb on + strandat 2684.575 kb on - strandat 2684.575 kb on - strandat 2684.575 kb on - strandat 2684.610 kb on - strandat 2684.641 kb on + strandat 2684.642 kb on - strandat 2684.674 kb on + strand, within PP_5520at 2684.674 kb on + strand, within PP_5520at 2684.674 kb on + strand, within PP_5520at 2684.674 kb on + strand, within PP_5520at 2684.730 kb on - strand, within PP_5520at 2684.780 kb on - strand, within PP_5520at 2684.780 kb on - strand, within PP_5520at 2684.780 kb on - strand, within PP_5520at 2684.912 kb on + strandat 2684.912 kb on + strandat 2685.052 kb on + strandat 2685.229 kb on - strandat 2685.236 kb on - strandat 2685.236 kb on - strandat 2685.236 kb on - strandat 2685.444 kb on - strandat 2685.634 kb on + strand, within PP_2354at 2685.635 kb on - strand, within PP_2354at 2685.673 kb on + strand, within PP_2354at 2685.674 kb on - strand, within PP_2354at 2685.674 kb on - strand, within PP_2354at 2685.752 kb on - strand, within PP_2354at 2685.765 kb on - strand, within PP_2354at 2685.765 kb on - strand, within PP_2354

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH7 and (C) Trisodium citrate
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2,683,651 + PP_2352 0.19 +0.2
2,683,651 + PP_2352 0.19 -0.3
2,683,672 + PP_2352 0.21 +0.8
2,683,672 + PP_2352 0.21 +0.9
2,683,672 + PP_2352 0.21 -0.6
2,683,776 + PP_2352 0.30 -0.2
2,683,840 + PP_2352 0.36 +0.4
2,683,840 + PP_2352 0.36 +0.3
2,683,840 + PP_2352 0.36 -0.8
2,683,840 + PP_2352 0.36 +0.0
2,683,840 + PP_2352 0.36 +1.7
2,683,841 - PP_2352 0.36 -2.8
2,683,841 - PP_2352 0.36 +0.7
2,683,841 - PP_2352 0.36 -1.0
2,683,841 - PP_2352 0.36 +0.6
2,683,841 - PP_2352 0.36 +0.6
2,683,841 - +0.2
2,683,841 - PP_2352 0.36 -0.5
2,683,853 + PP_2352 0.37 +0.5
2,684,007 - PP_2352 0.51 +0.8
2,684,023 + PP_2352 0.52 +0.0
2,684,024 - PP_2352 0.52 -0.1
2,684,024 - PP_2352 0.52 -0.7
2,684,080 - PP_2352 0.57 +0.7
2,684,080 - PP_2352 0.57 -1.5
2,684,299 + PP_2352 0.76 -0.8
2,684,300 - PP_2352 0.77 +1.0
2,684,306 - PP_2352 0.77 +0.4
2,684,571 + -0.7
2,684,571 + +1.6
2,684,575 - +0.5
2,684,575 - +0.2
2,684,575 - +0.3
2,684,610 - +0.8
2,684,641 + +1.0
2,684,642 - +0.4
2,684,674 + PP_5520 0.14 +1.0
2,684,674 + PP_5520 0.14 +1.2
2,684,674 + PP_5520 0.14 -0.2
2,684,674 + PP_5520 0.14 +0.4
2,684,730 - PP_5520 0.37 -0.3
2,684,780 - PP_5520 0.58 +0.0
2,684,780 - PP_5520 0.58 +1.7
2,684,780 - PP_5520 0.58 +1.2
2,684,912 + -0.6
2,684,912 + -0.4
2,685,052 + -1.6
2,685,229 - +2.0
2,685,236 - -0.6
2,685,236 - +0.0
2,685,236 - -1.8
2,685,444 - -0.1
2,685,634 + PP_2354 0.13 +0.8
2,685,635 - PP_2354 0.13 -1.0
2,685,673 + PP_2354 0.14 -0.3
2,685,674 - PP_2354 0.14 +0.3
2,685,674 - PP_2354 0.14 -0.3
2,685,752 - PP_2354 0.18 -0.8
2,685,765 - PP_2354 0.18 -0.5
2,685,765 - PP_2354 0.18 +0.5

Or see this region's nucleotide sequence