Experiment: Growth at pH7 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5014 and PP_5015 are separated by 1 nucleotides PP_5015 and PP_5016 are separated by 81 nucleotides PP_5016 and PP_5017 are separated by 3 nucleotides PP_5017 and PP_5018 overlap by 4 nucleotides PP_5018 and PP_5019 overlap by 4 nucleotides
PP_5014: PP_5014 - Phosphoribosyl-AMP cyclohydrolase, at 5,714,247 to 5,714,639
_5014
PP_5015: PP_5015 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) <small>(from data)</small>, at 5,714,641 to 5,714,976
_5015
PP_5016: PP_5016 - twin arginine protein export complex subunit, at 5,715,058 to 5,715,330
_5016
PP_5017: PP_5017 - Sec-independent protein translocase protein TatB, at 5,715,334 to 5,715,711
_5017
PP_5018: PP_5018 - sec-independent protein translocase protein, at 5,715,708 to 5,716,496
_5018
PP_5019: PP_5019 - Ribosomal RNA small subunit methyltransferase E, at 5,716,493 to 5,717,200
_5019
Position (kb) 5715
5716 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5714.395 kb on - strand, within PP_5014 at 5714.494 kb on + strand, within PP_5014 at 5714.523 kb on - strand, within PP_5014 at 5714.556 kb on + strand, within PP_5014 at 5714.557 kb on - strand, within PP_5014 at 5714.565 kb on + strand, within PP_5014 at 5714.566 kb on - strand, within PP_5014 at 5714.566 kb on - strand, within PP_5014 at 5714.622 kb on + strand at 5714.623 kb on - strand at 5714.623 kb on - strand at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.710 kb on + strand, within PP_5015 at 5714.711 kb on - strand, within PP_5015 at 5714.711 kb on - strand, within PP_5015 at 5714.725 kb on + strand at 5714.818 kb on + strand, within PP_5015 at 5714.834 kb on - strand, within PP_5015 at 5715.026 kb on + strand at 5715.033 kb on + strand at 5715.564 kb on + strand, within PP_5017 at 5715.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH7 and (C) Trisodium citrate remove 5,714,395 - PP_5014 0.38 -1.3 5,714,494 + PP_5014 0.63 -2.7 5,714,523 - PP_5014 0.70 -2.4 5,714,556 + PP_5014 0.79 -1.2 5,714,557 - PP_5014 0.79 -1.3 5,714,565 + PP_5014 0.81 -3.2 5,714,566 - PP_5014 0.81 -0.5 5,714,566 - PP_5014 0.81 -1.7 5,714,622 + -0.2 5,714,623 - -2.1 5,714,623 - -1.6 5,714,710 + PP_5015 0.21 -2.3 5,714,710 + PP_5015 0.21 -2.4 5,714,710 + PP_5015 0.21 -2.8 5,714,710 + PP_5015 0.21 -0.7 5,714,711 - PP_5015 0.21 +0.1 5,714,711 - PP_5015 0.21 +1.1 5,714,725 + +1.7 5,714,818 + PP_5015 0.53 -3.8 5,714,834 - PP_5015 0.57 -2.7 5,715,026 + +0.9 5,715,033 + -0.0 5,715,564 + PP_5017 0.61 +0.6 5,715,564 + +0.1
Or see this region's nucleotide sequence