Strain Fitness in Pseudomonas putida KT2440 around PP_1979

Experiment: Growth at pH7 and (C) Trisodium citrate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_t28 and PP_t29 are separated by 61 nucleotidesPP_t29 and PP_t30 are separated by 42 nucleotidesPP_t30 and PP_1978 are separated by 170 nucleotidesPP_1978 and PP_1979 are separated by 15 nucleotidesPP_1979 and PP_1980 are separated by 104 nucleotidesPP_1980 and PP_1981 are separated by 51 nucleotides PP_t28: PP_t28 - tRNA-Glu, at 2,241,927 to 2,242,002 _t28 PP_t29: PP_t29 - tRNA-Ala, at 2,242,064 to 2,242,139 _t29 PP_t30: PP_t30 - tRNA-Glu, at 2,242,182 to 2,242,257 _t30 PP_1978: PP_1978 - Transcriptional regulator, TetR family, at 2,242,428 to 2,242,967 _1978 PP_1979: PP_1979 - putative Hydrolase, at 2,242,983 to 2,243,864 _1979 PP_1980: PP_1980 - conserved protein of unknown function, at 2,243,969 to 2,244,403 _1980 PP_1981: PP_1981 - tRNA-dihydrouridine synthase C, at 2,244,455 to 2,245,426 _1981 Position (kb) 2242 2243 2244Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2242.026 kb on + strandat 2242.072 kb on - strand, within PP_t29at 2242.072 kb on - strandat 2242.072 kb on - strand, within PP_t29at 2242.072 kb on - strand, within PP_t29at 2242.129 kb on + strand, within PP_t29at 2242.180 kb on - strandat 2242.284 kb on - strandat 2242.379 kb on - strandat 2242.560 kb on + strand, within PP_1978at 2242.560 kb on + strand, within PP_1978at 2242.560 kb on + strand, within PP_1978at 2242.560 kb on + strand, within PP_1978at 2242.561 kb on - strand, within PP_1978at 2242.561 kb on - strand, within PP_1978at 2242.589 kb on + strand, within PP_1978at 2242.589 kb on + strand, within PP_1978at 2242.589 kb on + strand, within PP_1978at 2242.589 kb on + strand, within PP_1978at 2242.590 kb on - strand, within PP_1978at 2242.590 kb on - strand, within PP_1978at 2242.590 kb on - strand, within PP_1978at 2242.590 kb on - strand, within PP_1978at 2242.695 kb on + strand, within PP_1978at 2242.695 kb on + strand, within PP_1978at 2242.696 kb on - strand, within PP_1978at 2242.726 kb on + strand, within PP_1978at 2242.726 kb on + strand, within PP_1978at 2242.727 kb on - strand, within PP_1978at 2242.988 kb on + strandat 2242.989 kb on - strandat 2242.989 kb on - strandat 2243.139 kb on - strand, within PP_1979at 2243.139 kb on - strand, within PP_1979at 2243.264 kb on + strand, within PP_1979at 2243.265 kb on - strand, within PP_1979at 2243.265 kb on - strand, within PP_1979at 2243.798 kb on + strandat 2243.832 kb on - strandat 2243.886 kb on - strandat 2243.887 kb on + strandat 2243.887 kb on + strandat 2243.888 kb on - strandat 2244.510 kb on + strandat 2244.510 kb on + strandat 2244.510 kb on + strandat 2244.510 kb on + strandat 2244.511 kb on - strandat 2244.511 kb on - strandat 2244.511 kb on - strandat 2244.678 kb on + strand, within PP_1981at 2244.678 kb on + strand, within PP_1981at 2244.679 kb on - strand, within PP_1981

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH7 and (C) Trisodium citrate
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2,242,026 + -0.2
2,242,072 - PP_t29 0.11 +1.0
2,242,072 - -1.4
2,242,072 - PP_t29 0.11 +0.6
2,242,072 - PP_t29 0.11 +1.2
2,242,129 + PP_t29 0.86 -1.6
2,242,180 - -0.2
2,242,284 - +2.1
2,242,379 - -0.3
2,242,560 + PP_1978 0.24 +0.5
2,242,560 + PP_1978 0.24 +0.1
2,242,560 + PP_1978 0.24 -0.5
2,242,560 + PP_1978 0.24 -2.8
2,242,561 - PP_1978 0.25 +1.2
2,242,561 - PP_1978 0.25 -1.4
2,242,589 + PP_1978 0.30 -1.0
2,242,589 + PP_1978 0.30 +0.2
2,242,589 + PP_1978 0.30 -0.3
2,242,589 + PP_1978 0.30 +1.6
2,242,590 - PP_1978 0.30 -0.1
2,242,590 - PP_1978 0.30 +0.7
2,242,590 - PP_1978 0.30 -1.0
2,242,590 - PP_1978 0.30 +0.3
2,242,695 + PP_1978 0.49 -0.6
2,242,695 + PP_1978 0.49 -1.1
2,242,696 - PP_1978 0.50 +0.4
2,242,726 + PP_1978 0.55 -0.9
2,242,726 + PP_1978 0.55 -0.5
2,242,727 - PP_1978 0.55 +0.0
2,242,988 + +0.4
2,242,989 - -2.2
2,242,989 - +1.4
2,243,139 - PP_1979 0.18 +0.4
2,243,139 - PP_1979 0.18 +1.4
2,243,264 + PP_1979 0.32 +1.0
2,243,265 - PP_1979 0.32 -3.4
2,243,265 - PP_1979 0.32 -1.0
2,243,798 + +0.6
2,243,832 - +0.1
2,243,886 - +0.5
2,243,887 + -0.6
2,243,887 + -0.3
2,243,888 - -0.4
2,244,510 + +1.0
2,244,510 + +0.3
2,244,510 + +0.8
2,244,510 + -1.3
2,244,511 - -2.3
2,244,511 - +0.5
2,244,511 - +0.8
2,244,678 + PP_1981 0.23 -1.3
2,244,678 + PP_1981 0.23 -1.3
2,244,679 - PP_1981 0.23 +1.4

Or see this region's nucleotide sequence