Experiment: Growth at pH7 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5644 and PP_3899 are separated by 167 nucleotides PP_3899 and PP_3900 are separated by 8 nucleotides PP_3900 and PP_3901 are separated by 149 nucleotides PP_3901 and PP_3902 are separated by 118 nucleotides PP_3902 and PP_3903 overlap by 11 nucleotides
PP_5644: PP_5644 - conserved membrane protein of unknown function, at 4,414,325 to 4,414,891
_5644
PP_3899: PP_3899 - conserved protein of unknown function, hicB family, at 4,415,059 to 4,415,391
_3899
PP_3900: PP_3900 - hypothetical protein, at 4,415,400 to 4,415,648
_3900
PP_3901: PP_3901 - conserved protein of unknown function, at 4,415,798 to 4,416,058
_3901
PP_3902: PP_3902 - conserved protein of unknown function, at 4,416,177 to 4,416,485
_3902
PP_3903: PP_3903 - conserved protein of unknown function, at 4,416,475 to 4,416,639
_3903
Position (kb) 4415
4416 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4414.432 kb on - strand, within PP_5644 at 4414.437 kb on + strand, within PP_5644 at 4414.489 kb on - strand, within PP_5644 at 4414.814 kb on + strand, within PP_5644 at 4414.863 kb on - strand at 4414.863 kb on - strand at 4414.866 kb on - strand at 4414.869 kb on + strand at 4414.870 kb on - strand at 4414.874 kb on + strand at 4415.018 kb on + strand at 4415.018 kb on + strand at 4415.019 kb on - strand at 4415.078 kb on + strand at 4415.079 kb on - strand at 4415.079 kb on - strand at 4415.150 kb on - strand, within PP_3899 at 4415.150 kb on - strand, within PP_3899 at 4415.430 kb on + strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.434 kb on - strand, within PP_3900 at 4415.445 kb on - strand, within PP_3900 at 4415.450 kb on - strand, within PP_3900 at 4415.642 kb on + strand at 4415.643 kb on - strand at 4415.643 kb on - strand at 4415.736 kb on + strand at 4415.736 kb on + strand at 4415.737 kb on - strand at 4415.746 kb on + strand at 4415.746 kb on + strand at 4415.747 kb on - strand at 4415.775 kb on - strand at 4415.792 kb on - strand at 4415.792 kb on - strand at 4415.803 kb on + strand at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.994 kb on + strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4416.049 kb on - strand at 4416.107 kb on + strand at 4416.126 kb on + strand at 4416.433 kb on + strand, within PP_3902 at 4416.433 kb on + strand, within PP_3902 at 4416.433 kb on + strand, within PP_3902 at 4416.434 kb on - strand, within PP_3902 at 4416.434 kb on - strand, within PP_3902 at 4416.518 kb on + strand, within PP_3903 at 4416.518 kb on + strand, within PP_3903 at 4416.519 kb on - strand, within PP_3903 at 4416.551 kb on + strand, within PP_3903 at 4416.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH7 and (C) Trisodium citrate remove 4,414,432 - PP_5644 0.19 -3.1 4,414,437 + PP_5644 0.20 +1.3 4,414,489 - PP_5644 0.29 -3.4 4,414,814 + PP_5644 0.86 +2.0 4,414,863 - -2.5 4,414,863 - +1.2 4,414,866 - +0.5 4,414,869 + -1.6 4,414,870 - +0.1 4,414,874 + -3.2 4,415,018 + -2.9 4,415,018 + -2.8 4,415,019 - -1.5 4,415,078 + -3.6 4,415,079 - +0.5 4,415,079 - +0.2 4,415,150 - PP_3899 0.27 +0.0 4,415,150 - PP_3899 0.27 -3.4 4,415,430 + PP_3900 0.12 +0.4 4,415,431 - PP_3900 0.12 -0.1 4,415,431 - PP_3900 0.12 +0.8 4,415,431 - PP_3900 0.12 +0.3 4,415,433 + PP_3900 0.13 +2.0 4,415,433 + PP_3900 0.13 +2.3 4,415,434 - PP_3900 0.14 +0.7 4,415,445 - PP_3900 0.18 +2.4 4,415,450 - PP_3900 0.20 +0.6 4,415,642 + +2.6 4,415,643 - +0.0 4,415,643 - +0.9 4,415,736 + -0.2 4,415,736 + +0.5 4,415,737 - -1.8 4,415,746 + -1.6 4,415,746 + +2.2 4,415,747 - +1.3 4,415,775 - -3.0 4,415,792 - +0.5 4,415,792 - -3.1 4,415,803 + +0.8 4,415,924 + PP_3901 0.48 -2.4 4,415,924 + PP_3901 0.48 +0.2 4,415,924 + PP_3901 0.48 -2.5 4,415,925 - PP_3901 0.49 -0.8 4,415,925 - PP_3901 0.49 -1.5 4,415,994 + PP_3901 0.75 +0.8 4,415,995 - PP_3901 0.75 +2.0 4,415,995 - PP_3901 0.75 -0.9 4,415,995 - PP_3901 0.75 -1.1 4,415,995 - PP_3901 0.75 +2.1 4,416,049 - +2.3 4,416,107 + +1.3 4,416,126 + -0.6 4,416,433 + PP_3902 0.83 +1.3 4,416,433 + PP_3902 0.83 +1.4 4,416,433 + PP_3902 0.83 -1.2 4,416,434 - PP_3902 0.83 -0.1 4,416,434 - PP_3902 0.83 -0.7 4,416,518 + PP_3903 0.26 -1.5 4,416,518 + PP_3903 0.26 -2.0 4,416,519 - PP_3903 0.27 +0.7 4,416,551 + PP_3903 0.46 +0.6 4,416,637 + -1.4
Or see this region's nucleotide sequence