Experiment: Growth at pH6 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0284 and PP_5439 are separated by 131 nucleotides PP_5439 and PP_5440 are separated by 239 nucleotides PP_5440 and PP_t72 are separated by 188 nucleotides PP_t72 and PP_0285 are separated by 90 nucleotides PP_0285 and PP_0286 overlap by 4 nucleotides
PP_0284: PP_0284 - gamma-aminobutyrate permease, at 341,484 to 342,869
_0284
PP_5439: PP_5439 - conserved protein of unknown function, at 343,001 to 343,444
_5439
PP_5440: PP_5440 - conserved protein of unknown function, at 343,684 to 343,911
_5440
PP_t72: PP_t72 - tRNA-Phe, at 344,100 to 344,175
_t72
PP_0285: PP_0285 - Fe(2+)-trafficking protein, at 344,266 to 344,538
_0285
PP_0286: PP_0286 - adenine glycosylase, at 344,535 to 345,602
_0286
Position (kb) 343
344 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 342.794 kb on + strand at 342.795 kb on - strand at 342.989 kb on + strand at 342.989 kb on + strand at 342.990 kb on - strand at 342.990 kb on - strand at 342.991 kb on + strand at 342.991 kb on + strand at 342.991 kb on + strand at 342.991 kb on + strand at 342.992 kb on - strand at 342.992 kb on - strand at 342.992 kb on - strand at 342.992 kb on - strand at 342.992 kb on - strand at 343.057 kb on + strand, within PP_5439 at 343.059 kb on + strand, within PP_5439 at 343.059 kb on + strand, within PP_5439 at 343.060 kb on - strand, within PP_5439 at 343.060 kb on - strand, within PP_5439 at 343.060 kb on - strand, within PP_5439 at 343.158 kb on + strand, within PP_5439 at 343.158 kb on + strand, within PP_5439 at 343.159 kb on - strand, within PP_5439 at 343.314 kb on + strand, within PP_5439 at 343.314 kb on + strand, within PP_5439 at 343.315 kb on - strand, within PP_5439 at 343.373 kb on + strand, within PP_5439 at 343.479 kb on - strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.553 kb on - strand at 343.553 kb on - strand at 343.561 kb on + strand at 343.583 kb on + strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.697 kb on - strand at 343.717 kb on + strand, within PP_5440 at 343.717 kb on + strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.741 kb on + strand, within PP_5440 at 343.742 kb on - strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.780 kb on - strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.909 kb on + strand at 343.909 kb on + strand at 343.910 kb on - strand at 343.961 kb on + strand at 343.962 kb on - strand at 344.286 kb on - strand at 344.511 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.861 kb on - strand, within PP_0286
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) Trisodium citrate remove 342,794 + -1.9 342,795 - -2.0 342,989 + -1.7 342,989 + +0.7 342,990 - -0.5 342,990 - -0.4 342,991 + -2.7 342,991 + +0.9 342,991 + -0.5 342,991 + +0.7 342,992 - -1.3 342,992 - +2.1 342,992 - +0.7 342,992 - +0.9 342,992 - -1.1 343,057 + PP_5439 0.13 -0.0 343,059 + PP_5439 0.13 -2.4 343,059 + PP_5439 0.13 -3.6 343,060 - PP_5439 0.13 +0.8 343,060 - PP_5439 0.13 +1.4 343,060 - PP_5439 0.13 +0.7 343,158 + PP_5439 0.35 +0.6 343,158 + PP_5439 0.35 -2.2 343,159 - PP_5439 0.36 -3.0 343,314 + PP_5439 0.70 +0.9 343,314 + PP_5439 0.70 -0.2 343,315 - PP_5439 0.71 -0.3 343,373 + PP_5439 0.84 -1.6 343,479 - +0.2 343,548 + -0.6 343,548 + +0.6 343,548 + -0.2 343,548 + -1.7 343,548 + +0.4 343,548 + -3.5 343,549 - -0.4 343,549 - +0.5 343,549 - -0.6 343,553 - -1.9 343,553 - +0.9 343,561 + -2.5 343,583 + -2.5 343,584 - -2.6 343,584 - +1.5 343,584 - -1.4 343,584 - -0.9 343,669 + +1.8 343,669 + -3.0 343,697 - +3.1 343,717 + PP_5440 0.14 -0.6 343,717 + PP_5440 0.14 -1.7 343,739 - PP_5440 0.24 +1.8 343,739 - PP_5440 0.24 -3.1 343,739 - PP_5440 0.24 -0.7 343,739 - PP_5440 0.24 -1.1 343,741 + PP_5440 0.25 -0.3 343,742 - PP_5440 0.25 -0.3 343,779 + PP_5440 0.42 -2.3 343,779 + PP_5440 0.42 -3.9 343,780 - PP_5440 0.42 -0.0 343,852 + PP_5440 0.74 -2.7 343,852 + PP_5440 0.74 -0.8 343,853 - PP_5440 0.74 -0.5 343,853 - PP_5440 0.74 +1.3 343,853 - PP_5440 0.74 +0.0 343,909 + +0.4 343,909 + +1.4 343,910 - -0.4 343,961 + -3.4 343,962 - +0.1 344,286 - +0.6 344,511 - -1.9 344,633 - -3.6 344,633 - +1.0 344,633 - -2.1 344,633 - +0.8 344,861 - PP_0286 0.31 -1.2
Or see this region's nucleotide sequence