Experiment: Growth with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2745 and PP_2746 are separated by 337 nucleotides PP_2746 and PP_2747 are separated by 20 nucleotides PP_2747 and PP_2748 overlap by 34 nucleotides
PP_2745: PP_2745 - Universal stress protein family, at 3,127,520 to 3,128,416
_2745
PP_2746: PP_2746 - dimethyl-sulfide monooxygenase, at 3,128,754 to 3,130,163
_2746
PP_2747: PP_2747 - conserved protein of unknown function, at 3,130,184 to 3,131,155
_2747
PP_2748: PP_2748 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,131,122 to 3,131,922
_2748
Position (kb)
3128
3129
3130
3131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3127.807 kb on - strand, within PP_2745 at 3127.833 kb on + strand, within PP_2745 at 3127.955 kb on + strand, within PP_2745 at 3128.019 kb on - strand, within PP_2745 at 3128.410 kb on + strand at 3128.410 kb on + strand at 3128.411 kb on - strand at 3128.411 kb on - strand at 3128.500 kb on + strand at 3128.501 kb on - strand at 3128.504 kb on + strand at 3128.529 kb on - strand at 3128.592 kb on + strand at 3128.592 kb on + strand at 3128.673 kb on + strand at 3128.850 kb on + strand at 3128.940 kb on + strand, within PP_2746 at 3128.941 kb on - strand, within PP_2746 at 3128.941 kb on - strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.166 kb on - strand, within PP_2746 at 3129.231 kb on + strand, within PP_2746 at 3129.244 kb on - strand, within PP_2746 at 3129.258 kb on + strand, within PP_2746 at 3129.259 kb on - strand, within PP_2746 at 3129.426 kb on + strand, within PP_2746 at 3129.427 kb on - strand, within PP_2746 at 3129.658 kb on - strand, within PP_2746 at 3129.658 kb on - strand, within PP_2746 at 3129.659 kb on + strand, within PP_2746 at 3129.659 kb on + strand, within PP_2746 at 3129.660 kb on - strand, within PP_2746 at 3129.660 kb on - strand at 3129.666 kb on + strand, within PP_2746 at 3129.746 kb on + strand, within PP_2746 at 3129.752 kb on + strand, within PP_2746 at 3129.753 kb on - strand, within PP_2746 at 3129.842 kb on + strand, within PP_2746 at 3129.842 kb on + strand, within PP_2746 at 3130.018 kb on - strand, within PP_2746 at 3130.191 kb on + strand at 3130.191 kb on + strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.192 kb on - strand at 3130.221 kb on + strand at 3130.570 kb on - strand, within PP_2747 at 3130.807 kb on - strand at 3130.825 kb on - strand, within PP_2747 at 3131.080 kb on + strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.081 kb on - strand at 3131.101 kb on - strand at 3131.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 200 mM remove 3,127,807 - PP_2745 0.32 +0.9 3,127,833 + PP_2745 0.35 -0.4 3,127,955 + PP_2745 0.48 -1.4 3,128,019 - PP_2745 0.56 +2.9 3,128,410 + +0.5 3,128,410 + +0.5 3,128,411 - -0.4 3,128,411 - +1.6 3,128,500 + -0.2 3,128,501 - +0.2 3,128,504 + -1.1 3,128,529 - -0.8 3,128,592 + +0.4 3,128,592 + +0.4 3,128,673 + +0.8 3,128,850 + +0.5 3,128,940 + PP_2746 0.13 -1.0 3,128,941 - PP_2746 0.13 -0.6 3,128,941 - PP_2746 0.13 -0.2 3,129,158 + PP_2746 0.29 +0.5 3,129,158 + PP_2746 0.29 -0.8 3,129,158 + PP_2746 0.29 -0.2 3,129,166 - PP_2746 0.29 +1.2 3,129,231 + PP_2746 0.34 -1.0 3,129,244 - PP_2746 0.35 -1.6 3,129,258 + PP_2746 0.36 +0.5 3,129,259 - PP_2746 0.36 -2.7 3,129,426 + PP_2746 0.48 +0.5 3,129,427 - PP_2746 0.48 -0.1 3,129,658 - PP_2746 0.64 -0.7 3,129,658 - PP_2746 0.64 -0.8 3,129,659 + PP_2746 0.64 -2.7 3,129,659 + PP_2746 0.64 -4.1 3,129,660 - PP_2746 0.64 -0.7 3,129,660 - -1.8 3,129,666 + PP_2746 0.65 -0.8 3,129,746 + PP_2746 0.70 -4.1 3,129,752 + PP_2746 0.71 -0.6 3,129,753 - PP_2746 0.71 -0.7 3,129,842 + PP_2746 0.77 -3.8 3,129,842 + PP_2746 0.77 -3.8 3,130,018 - PP_2746 0.90 +1.6 3,130,191 + +0.0 3,130,191 + -1.0 3,130,192 - -0.2 3,130,192 - -1.4 3,130,192 - +1.2 3,130,192 - +0.5 3,130,221 + +1.2 3,130,570 - PP_2747 0.40 -0.0 3,130,807 - +0.7 3,130,825 - PP_2747 0.66 -0.7 3,131,080 + +1.7 3,131,081 - +1.6 3,131,081 - +1.2 3,131,081 - +0.1 3,131,081 - +0.8 3,131,101 - -2.9 3,131,135 + +0.8
Or see this region's nucleotide sequence