Strain Fitness in Pseudomonas simiae WCS417 around PS417_00015

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00010 and PS417_00015 are separated by 20 nucleotidesPS417_00015 and PS417_00020 are separated by 4 nucleotides PS417_00010: PS417_00010 - DNA polymerase III subunit beta, at 1,781 to 2,884 _00010 PS417_00015: PS417_00015 - recombinase RecF, at 2,905 to 4,008 _00015 PS417_00020: PS417_00020 - DNA gyrase subunit B, at 4,013 to 6,430 _00020 Position (kb) 2 3 4 5Strain fitness (log2 ratio) -3 -2 -1 0 1at 2.902 kb on + strandat 2.902 kb on + strandat 2.902 kb on + strandat 2.903 kb on - strandat 2.903 kb on - strandat 3.147 kb on + strand, within PS417_00015at 3.148 kb on - strand, within PS417_00015at 3.148 kb on - strand, within PS417_00015at 3.473 kb on + strand, within PS417_00015at 3.473 kb on + strand, within PS417_00015at 3.493 kb on + strand, within PS417_00015at 3.493 kb on + strand, within PS417_00015at 3.495 kb on + strand, within PS417_00015at 3.495 kb on + strand, within PS417_00015at 3.495 kb on + strand, within PS417_00015at 3.684 kb on + strand, within PS417_00015at 3.801 kb on + strand, within PS417_00015at 3.801 kb on + strand, within PS417_00015at 3.801 kb on + strand, within PS417_00015at 3.801 kb on + strand, within PS417_00015at 3.805 kb on + strand, within PS417_00015at 3.878 kb on + strand, within PS417_00015at 3.878 kb on + strand, within PS417_00015at 3.917 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
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2,902 + -0.3
2,902 + -1.7
2,902 + -2.9
2,903 - -0.6
2,903 - -2.0
3,147 + PS417_00015 0.22 -0.2
3,148 - PS417_00015 0.22 -2.1
3,148 - PS417_00015 0.22 -0.1
3,473 + PS417_00015 0.51 -0.3
3,473 + PS417_00015 0.51 -3.0
3,493 + PS417_00015 0.53 +1.3
3,493 + PS417_00015 0.53 -1.2
3,495 + PS417_00015 0.53 -1.0
3,495 + PS417_00015 0.53 -0.1
3,495 + PS417_00015 0.53 -0.8
3,684 + PS417_00015 0.71 -2.4
3,801 + PS417_00015 0.81 -0.1
3,801 + PS417_00015 0.81 -1.9
3,801 + PS417_00015 0.81 +1.1
3,801 + PS417_00015 0.81 -2.0
3,805 + PS417_00015 0.82 +0.6
3,878 + PS417_00015 0.88 -0.7
3,878 + PS417_00015 0.88 -1.3
3,917 + -0.8

Or see this region's nucleotide sequence