Strain Fitness in Pseudomonas simiae WCS417 around PS417_17550

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_17545 and PS417_17550 are separated by 1 nucleotidesPS417_17550 and PS417_17555 are separated by 0 nucleotides PS417_17545: PS417_17545 - 2-oxoisovalerate dehydrogenase, at 3,831,243 to 3,832,478 _17545 PS417_17550: PS417_17550 - branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit (EC 1.2.4.4) (from data), at 3,832,480 to 3,833,538 _17550 PS417_17555: PS417_17555 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 3,833,539 to 3,834,798 _17555 Position (kb) 3832 3833 3834Strain fitness (log2 ratio) -3 -2 -1 0 1at 3831.561 kb on + strand, within PS417_17545at 3831.643 kb on - strand, within PS417_17545at 3831.754 kb on - strand, within PS417_17545at 3831.812 kb on - strand, within PS417_17545at 3831.934 kb on + strand, within PS417_17545at 3832.206 kb on + strand, within PS417_17545at 3832.780 kb on + strand, within PS417_17550at 3832.780 kb on + strand, within PS417_17550at 3832.895 kb on - strand, within PS417_17550at 3833.137 kb on + strandat 3833.138 kb on - strand, within PS417_17550at 3833.215 kb on + strand, within PS417_17550at 3833.215 kb on + strandat 3833.215 kb on + strand, within PS417_17550at 3833.483 kb on - strandat 3834.194 kb on + strand, within PS417_17555

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
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3,831,561 + PS417_17545 0.26 +0.7
3,831,643 - PS417_17545 0.32 +0.6
3,831,754 - PS417_17545 0.41 -2.8
3,831,812 - PS417_17545 0.46 +0.2
3,831,934 + PS417_17545 0.56 -3.1
3,832,206 + PS417_17545 0.78 -3.4
3,832,780 + PS417_17550 0.28 -0.4
3,832,780 + PS417_17550 0.28 -2.3
3,832,895 - PS417_17550 0.39 -0.3
3,833,137 + -0.4
3,833,138 - PS417_17550 0.62 -0.9
3,833,215 + PS417_17550 0.69 -1.7
3,833,215 + -1.8
3,833,215 + PS417_17550 0.69 +0.2
3,833,483 - -1.3
3,834,194 + PS417_17555 0.52 -1.4

Or see this region's nucleotide sequence