Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14630 and PS417_14635 overlap by 4 nucleotides PS417_14635 and PS417_14640 are separated by 385 nucleotides
PS417_14630: PS417_14630 - 3-demethylubiquinone-9 3-methyltransferase, at 3,195,413 to 3,195,895
_14630
PS417_14635: PS417_14635 - GDSL family lipase, at 3,195,892 to 3,196,788
_14635
PS417_14640: PS417_14640 - dihydrodipicolinate synthase, at 3,197,174 to 3,198,037
_14640
Position (kb)
3195
3196
3197 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3194.959 kb on + strand at 3194.959 kb on + strand at 3194.960 kb on - strand at 3195.014 kb on + strand at 3195.014 kb on + strand at 3195.015 kb on - strand at 3195.050 kb on + strand at 3195.051 kb on - strand at 3195.103 kb on + strand at 3195.106 kb on + strand at 3195.171 kb on + strand at 3195.217 kb on + strand at 3195.217 kb on + strand at 3195.217 kb on + strand at 3195.318 kb on + strand at 3195.319 kb on - strand at 3195.378 kb on - strand at 3195.559 kb on - strand, within PS417_14630 at 3195.965 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3196.100 kb on + strand, within PS417_14635 at 3196.100 kb on + strand, within PS417_14635 at 3196.166 kb on + strand, within PS417_14635 at 3196.386 kb on - strand, within PS417_14635 at 3196.487 kb on + strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.718 kb on + strand at 3196.719 kb on - strand at 3196.813 kb on + strand at 3196.880 kb on + strand at 3196.915 kb on + strand at 3197.191 kb on + strand at 3197.191 kb on + strand at 3197.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 remove 3,194,959 + -0.9 3,194,959 + -0.6 3,194,960 - -2.2 3,195,014 + +0.7 3,195,014 + +0.3 3,195,015 - -0.8 3,195,050 + -0.6 3,195,051 - +0.3 3,195,103 + -0.2 3,195,106 + -1.6 3,195,171 + -0.4 3,195,217 + +0.0 3,195,217 + -0.7 3,195,217 + +0.3 3,195,318 + -0.4 3,195,319 - +0.2 3,195,378 - -3.0 3,195,559 - PS417_14630 0.30 +0.6 3,195,965 + -0.2 3,195,968 + -1.4 3,195,968 + +1.6 3,195,968 + +0.2 3,195,968 + -0.8 3,196,100 + PS417_14635 0.23 +1.0 3,196,100 + PS417_14635 0.23 +0.7 3,196,166 + PS417_14635 0.31 +0.4 3,196,386 - PS417_14635 0.55 +0.3 3,196,487 + PS417_14635 0.66 -0.1 3,196,488 - PS417_14635 0.66 +1.0 3,196,488 - PS417_14635 0.66 +0.2 3,196,718 + -0.5 3,196,719 - +0.1 3,196,813 + +1.2 3,196,880 + -0.1 3,196,915 + -2.6 3,197,191 + +0.1 3,197,191 + -1.7 3,197,192 - -0.4
Or see this region's nucleotide sequence