Strain Fitness in Pseudomonas simiae WCS417 around PS417_14355

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_14345 and PS417_14350 are separated by 75 nucleotidesPS417_14350 and PS417_14355 are separated by 30 nucleotidesPS417_14355 and PS417_14360 are separated by 100 nucleotidesPS417_14360 and PS417_14365 are separated by 34 nucleotidesPS417_14365 and PS417_14370 are separated by 38 nucleotides PS417_14345: PS417_14345 - LysR family transcriptional regulator, at 3,138,625 to 3,139,530 _14345 PS417_14350: PS417_14350 - multidrug DMT transporter permease, at 3,139,606 to 3,140,433 _14350 PS417_14355: PS417_14355 - sugar dehydrogenase, at 3,140,464 to 3,141,225 _14355 PS417_14360: PS417_14360 - hypothetical protein, at 3,141,326 to 3,141,868 _14360 PS417_14365: PS417_14365 - hypothetical protein, at 3,141,903 to 3,142,157 _14365 PS417_14370: PS417_14370 - Pathogenicity locus, at 3,142,196 to 3,142,429 _14370 Position (kb) 3140 3141 3142Strain fitness (log2 ratio) -2 -1 0 1 2at 3139.617 kb on + strandat 3139.622 kb on + strandat 3139.623 kb on - strandat 3139.626 kb on - strandat 3139.754 kb on - strand, within PS417_14350at 3139.820 kb on - strand, within PS417_14350at 3139.822 kb on - strand, within PS417_14350at 3139.822 kb on - strand, within PS417_14350at 3139.986 kb on + strand, within PS417_14350at 3140.119 kb on + strand, within PS417_14350at 3140.119 kb on + strand, within PS417_14350at 3140.167 kb on + strand, within PS417_14350at 3140.168 kb on - strand, within PS417_14350at 3140.272 kb on + strand, within PS417_14350at 3140.272 kb on + strand, within PS417_14350at 3140.273 kb on - strand, within PS417_14350at 3140.273 kb on - strand, within PS417_14350at 3140.351 kb on - strandat 3140.575 kb on + strand, within PS417_14355at 3140.648 kb on + strand, within PS417_14355at 3140.938 kb on + strand, within PS417_14355at 3140.939 kb on - strand, within PS417_14355at 3140.939 kb on - strand, within PS417_14355at 3140.948 kb on - strand, within PS417_14355at 3141.046 kb on - strand, within PS417_14355at 3141.046 kb on - strand, within PS417_14355at 3141.046 kb on - strand, within PS417_14355at 3141.186 kb on + strandat 3141.187 kb on - strandat 3141.209 kb on + strandat 3141.209 kb on + strandat 3141.224 kb on - strandat 3141.232 kb on + strandat 3141.232 kb on + strandat 3141.233 kb on - strandat 3141.308 kb on - strandat 3141.444 kb on + strand, within PS417_14360at 3141.445 kb on - strand, within PS417_14360at 3141.445 kb on - strand, within PS417_14360at 3141.518 kb on + strand, within PS417_14360at 3141.518 kb on + strand, within PS417_14360at 3141.519 kb on - strand, within PS417_14360at 3141.528 kb on - strand, within PS417_14360at 3141.563 kb on + strand, within PS417_14360at 3141.669 kb on - strand, within PS417_14360at 3141.745 kb on - strand, within PS417_14360at 3141.787 kb on + strandat 3141.821 kb on + strandat 3141.900 kb on + strandat 3141.900 kb on + strandat 3141.901 kb on - strandat 3142.108 kb on - strand, within PS417_14365at 3142.156 kb on - strandat 3142.159 kb on + strandat 3142.160 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
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3,139,617 + -2.1
3,139,622 + -2.3
3,139,623 - +0.4
3,139,626 - -1.4
3,139,754 - PS417_14350 0.18 -0.0
3,139,820 - PS417_14350 0.26 +0.1
3,139,822 - PS417_14350 0.26 +0.5
3,139,822 - PS417_14350 0.26 -0.1
3,139,986 + PS417_14350 0.46 -2.2
3,140,119 + PS417_14350 0.62 -1.9
3,140,119 + PS417_14350 0.62 +0.4
3,140,167 + PS417_14350 0.68 -0.4
3,140,168 - PS417_14350 0.68 -0.4
3,140,272 + PS417_14350 0.80 -0.5
3,140,272 + PS417_14350 0.80 -0.0
3,140,273 - PS417_14350 0.81 -0.2
3,140,273 - PS417_14350 0.81 +0.1
3,140,351 - +0.1
3,140,575 + PS417_14355 0.15 +1.1
3,140,648 + PS417_14355 0.24 -0.0
3,140,938 + PS417_14355 0.62 +0.0
3,140,939 - PS417_14355 0.62 +1.4
3,140,939 - PS417_14355 0.62 -0.7
3,140,948 - PS417_14355 0.64 +0.6
3,141,046 - PS417_14355 0.76 +2.2
3,141,046 - PS417_14355 0.76 +0.0
3,141,046 - PS417_14355 0.76 +0.5
3,141,186 + -0.5
3,141,187 - +2.7
3,141,209 + -0.0
3,141,209 + +0.7
3,141,224 - +0.3
3,141,232 + -0.5
3,141,232 + -0.5
3,141,233 - -0.4
3,141,308 - -0.4
3,141,444 + PS417_14360 0.22 +1.2
3,141,445 - PS417_14360 0.22 -0.2
3,141,445 - PS417_14360 0.22 -0.8
3,141,518 + PS417_14360 0.35 -0.5
3,141,518 + PS417_14360 0.35 -0.1
3,141,519 - PS417_14360 0.36 +0.1
3,141,528 - PS417_14360 0.37 -1.2
3,141,563 + PS417_14360 0.44 +1.3
3,141,669 - PS417_14360 0.63 +1.1
3,141,745 - PS417_14360 0.77 -1.0
3,141,787 + +1.3
3,141,821 + +0.2
3,141,900 + +0.2
3,141,900 + +0.9
3,141,901 - +1.5
3,142,108 - PS417_14365 0.80 -0.2
3,142,156 - +0.8
3,142,159 + +0.2
3,142,160 - +0.8

Or see this region's nucleotide sequence