Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06585 and PS417_06590 are separated by 23 nucleotides PS417_06590 and PS417_06595 are separated by 260 nucleotides PS417_06595 and PS417_06600 are separated by 1 nucleotides PS417_06600 and PS417_06605 overlap by 4 nucleotides
PS417_06585: PS417_06585 - hypothetical protein, at 1,458,384 to 1,459,262
_06585
PS417_06590: PS417_06590 - membrane protein, at 1,459,286 to 1,459,582
_06590
PS417_06595: PS417_06595 - ABC transporter ATP-binding protein, at 1,459,843 to 1,460,544
_06595
PS417_06600: PS417_06600 - leucine/isoleucine/valine transporter ATP-binding subunit, at 1,460,546 to 1,461,313
_06600
PS417_06605: PS417_06605 - leucine/isoleucine/valine transporter permease subunit, at 1,461,310 to 1,462,563
_06605
Position (kb)
1459
1460
1461 Strain fitness (log2 ratio)
-1
0
1 at 1459.058 kb on - strand, within PS417_06585 at 1459.081 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.267 kb on - strand at 1459.639 kb on - strand at 1460.367 kb on - strand, within PS417_06595 at 1460.513 kb on + strand at 1460.547 kb on + strand at 1460.563 kb on - strand at 1461.151 kb on - strand, within PS417_06600
Per-strain Table
Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 remove 1,459,058 - PS417_06585 0.77 +0.5 1,459,081 + PS417_06585 0.79 -0.1 1,459,111 + PS417_06585 0.83 -0.4 1,459,111 + +0.6 1,459,111 + PS417_06585 0.83 -1.7 1,459,111 + PS417_06585 0.83 -0.0 1,459,111 + PS417_06585 0.83 -0.3 1,459,267 - -0.5 1,459,639 - -0.8 1,460,367 - PS417_06595 0.75 -0.5 1,460,513 + -0.6 1,460,547 + +0.6 1,460,563 - +0.6 1,461,151 - PS417_06600 0.79 -1.6
Or see this region's nucleotide sequence