Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02220 and PS417_02225 are separated by 383 nucleotides PS417_02225 and PS417_02230 are separated by 54 nucleotides
PS417_02220: PS417_02220 - pyruvate dehydrogenase, at 502,327 to 504,972
_02220
PS417_02225: PS417_02225 - bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase, at 505,356 to 508,295
_02225
PS417_02230: PS417_02230 - ADP-heptose--LPS heptosyltransferase, at 508,350 to 509,384
_02230
Position (kb)
505
506
507
508
509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 505.128 kb on - strand at 505.132 kb on - strand at 505.138 kb on - strand at 505.297 kb on - strand at 505.311 kb on - strand at 505.323 kb on - strand at 506.676 kb on + strand, within PS417_02225 at 506.677 kb on - strand, within PS417_02225 at 507.039 kb on + strand, within PS417_02225 at 508.301 kb on + strand at 508.319 kb on + strand at 508.319 kb on + strand at 508.347 kb on + strand at 508.347 kb on + strand at 508.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 remove 505,128 - +0.8 505,132 - -0.8 505,138 - -0.2 505,297 - +0.2 505,311 - -0.7 505,323 - -0.8 506,676 + PS417_02225 0.45 -2.7 506,677 - PS417_02225 0.45 -1.8 507,039 + PS417_02225 0.57 -1.4 508,301 + -0.3 508,319 + +0.3 508,319 + +1.8 508,347 + +0.6 508,347 + +1.0 508,347 + +0.7
Or see this region's nucleotide sequence