Experiment: Temperature shift 10_to_34; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4737 and PP_4738 are separated by 319 nucleotides PP_4738 and PP_4739 are separated by 857 nucleotides
PP_4737: PP_4737 - D-lactate dehydrogenase, at 5,386,491 to 5,389,301
_4737
PP_4738: PP_4738 - conserved exported protein of unknown function, at 5,389,621 to 5,389,911
_4738
PP_4739: PP_4739 - conserved protein of unknown function, at 5,390,769 to 5,391,011
_4739
Position (kb)
5389
5390 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5388.642 kb on + strand, within PP_4737 at 5388.642 kb on + strand, within PP_4737 at 5388.642 kb on + strand, within PP_4737 at 5388.852 kb on + strand, within PP_4737 at 5388.853 kb on - strand, within PP_4737 at 5388.919 kb on - strand, within PP_4737 at 5388.919 kb on - strand, within PP_4737 at 5389.088 kb on + strand at 5389.088 kb on + strand at 5389.089 kb on - strand at 5389.124 kb on + strand at 5389.124 kb on + strand at 5389.125 kb on - strand at 5389.180 kb on - strand at 5389.194 kb on - strand at 5389.248 kb on + strand at 5389.249 kb on - strand at 5389.249 kb on - strand at 5389.263 kb on + strand at 5389.263 kb on + strand at 5389.264 kb on - strand at 5389.310 kb on + strand at 5389.311 kb on - strand at 5389.311 kb on - strand at 5389.311 kb on - strand at 5389.312 kb on + strand at 5389.312 kb on + strand at 5389.313 kb on - strand at 5389.313 kb on - strand at 5389.313 kb on - strand at 5389.313 kb on - strand at 5389.330 kb on + strand at 5389.331 kb on - strand at 5389.342 kb on + strand at 5389.416 kb on - strand at 5389.462 kb on + strand at 5389.462 kb on + strand at 5389.462 kb on + strand at 5389.463 kb on - strand at 5389.485 kb on + strand at 5389.521 kb on + strand at 5389.521 kb on + strand at 5389.521 kb on + strand at 5389.522 kb on - strand at 5389.583 kb on + strand at 5389.583 kb on + strand at 5389.584 kb on - strand at 5389.584 kb on - strand at 5389.842 kb on + strand, within PP_4738 at 5389.842 kb on + strand, within PP_4738 at 5389.843 kb on - strand, within PP_4738 at 5389.843 kb on - strand, within PP_4738 at 5389.843 kb on - strand, within PP_4738 at 5389.963 kb on + strand at 5389.965 kb on + strand at 5389.984 kb on + strand at 5390.501 kb on + strand at 5390.519 kb on + strand at 5390.519 kb on + strand at 5390.520 kb on - strand at 5390.520 kb on - strand at 5390.520 kb on - strand at 5390.520 kb on - strand at 5390.520 kb on - strand at 5390.565 kb on - strand at 5390.565 kb on - strand at 5390.617 kb on + strand at 5390.617 kb on + strand at 5390.618 kb on - strand at 5390.618 kb on - strand at 5390.631 kb on - strand at 5390.631 kb on - strand at 5390.765 kb on + strand at 5390.765 kb on + strand at 5390.773 kb on + strand at 5390.773 kb on + strand at 5390.878 kb on + strand, within PP_4739 at 5390.878 kb on + strand, within PP_4739
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_34; with MOPS remove 5,388,642 + PP_4737 0.77 +0.0 5,388,642 + PP_4737 0.77 +0.7 5,388,642 + PP_4737 0.77 -0.1 5,388,852 + PP_4737 0.84 -0.6 5,388,853 - PP_4737 0.84 +1.1 5,388,919 - PP_4737 0.86 +0.5 5,388,919 - PP_4737 0.86 -0.6 5,389,088 + +1.1 5,389,088 + -2.1 5,389,089 - -0.1 5,389,124 + -3.2 5,389,124 + +1.2 5,389,125 - -0.4 5,389,180 - +0.5 5,389,194 - -0.6 5,389,248 + -2.6 5,389,249 - +0.0 5,389,249 - +0.5 5,389,263 + -1.2 5,389,263 + -0.5 5,389,264 - -0.0 5,389,310 + +1.3 5,389,311 - -2.1 5,389,311 - -0.1 5,389,311 - +0.4 5,389,312 + +0.2 5,389,312 + +1.3 5,389,313 - +0.2 5,389,313 - -0.3 5,389,313 - +1.0 5,389,313 - +1.4 5,389,330 + +1.1 5,389,331 - +1.1 5,389,342 + -1.4 5,389,416 - -0.3 5,389,462 + +1.8 5,389,462 + -0.9 5,389,462 + +0.8 5,389,463 - +0.7 5,389,485 + -0.3 5,389,521 + -1.1 5,389,521 + +0.5 5,389,521 + +0.4 5,389,522 - -0.2 5,389,583 + -0.6 5,389,583 + -1.6 5,389,584 - +0.4 5,389,584 - -0.0 5,389,842 + PP_4738 0.76 -0.7 5,389,842 + PP_4738 0.76 -1.1 5,389,843 - PP_4738 0.76 +0.1 5,389,843 - PP_4738 0.76 -1.5 5,389,843 - PP_4738 0.76 -1.1 5,389,963 + +2.1 5,389,965 + +2.1 5,389,984 + +0.5 5,390,501 + -1.2 5,390,519 + -0.4 5,390,519 + -0.2 5,390,520 - -0.9 5,390,520 - +0.7 5,390,520 - -2.1 5,390,520 - -0.4 5,390,520 - +1.3 5,390,565 - +0.1 5,390,565 - -0.0 5,390,617 + +0.9 5,390,617 + -0.5 5,390,618 - -0.7 5,390,618 - +0.1 5,390,631 - +0.2 5,390,631 - -0.3 5,390,765 + +0.5 5,390,765 + -1.8 5,390,773 + +0.9 5,390,773 + +1.3 5,390,878 + PP_4739 0.45 -0.1 5,390,878 + PP_4739 0.45 -0.4
Or see this region's nucleotide sequence