Strain Fitness in Pseudomonas simiae WCS417 around PS417_00315

Experiment: Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00305 and PS417_00310 are separated by 21 nucleotidesPS417_00310 and PS417_00315 are separated by 35 nucleotidesPS417_00315 and PS417_00320 are separated by 6 nucleotidesPS417_00320 and PS417_00325 are separated by 28 nucleotides PS417_00305: PS417_00305 - cytochrome oxidase subunit I, at 60,292 to 61,878 _00305 PS417_00310: PS417_00310 - cysteine synthase, at 61,900 to 62,451 _00310 PS417_00315: PS417_00315 - MFS transporter, at 62,487 to 63,374 _00315 PS417_00320: PS417_00320 - hypothetical protein, at 63,381 to 63,584 _00320 PS417_00325: PS417_00325 - hypothetical protein, at 63,613 to 64,377 _00325 PS417_00330: PS417_00330 - hypothetical protein, at 64,343 to 64,927 _00330 Position (kb) 62 63 64Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 61.517 kb on - strand, within PS417_00305at 61.519 kb on + strand, within PS417_00305at 61.520 kb on - strand, within PS417_00305at 61.549 kb on + strand, within PS417_00305at 61.550 kb on - strand, within PS417_00305at 61.663 kb on + strand, within PS417_00305at 61.994 kb on - strand, within PS417_00310at 61.994 kb on - strand, within PS417_00310at 62.151 kb on - strand, within PS417_00310at 62.238 kb on + strand, within PS417_00310at 62.239 kb on - strand, within PS417_00310at 62.239 kb on - strand, within PS417_00310at 62.264 kb on - strand, within PS417_00310at 62.264 kb on - strand, within PS417_00310at 62.264 kb on - strand, within PS417_00310at 62.344 kb on - strand, within PS417_00310at 62.449 kb on + strandat 62.453 kb on + strandat 62.505 kb on + strandat 62.505 kb on + strandat 62.505 kb on + strandat 62.506 kb on - strandat 62.508 kb on + strandat 62.537 kb on - strandat 62.537 kb on - strandat 62.537 kb on - strandat 62.661 kb on + strand, within PS417_00315at 62.816 kb on - strand, within PS417_00315at 62.931 kb on + strand, within PS417_00315at 63.124 kb on - strand, within PS417_00315at 63.124 kb on - strand, within PS417_00315at 63.171 kb on + strand, within PS417_00315at 63.172 kb on - strand, within PS417_00315at 63.234 kb on - strand, within PS417_00315at 63.364 kb on - strandat 63.364 kb on - strandat 63.364 kb on - strandat 63.624 kb on + strandat 63.624 kb on + strandat 63.625 kb on - strandat 63.625 kb on - strandat 63.625 kb on - strandat 63.625 kb on - strandat 63.625 kb on - strandat 63.627 kb on - strandat 63.638 kb on + strandat 63.638 kb on + strandat 63.639 kb on - strandat 63.760 kb on + strand, within PS417_00325at 63.761 kb on - strand, within PS417_00325at 63.761 kb on - strand, within PS417_00325at 63.761 kb on - strand, within PS417_00325at 63.761 kb on - strand, within PS417_00325at 64.051 kb on + strand, within PS417_00325at 64.052 kb on - strand, within PS417_00325at 64.052 kb on - strand, within PS417_00325at 64.174 kb on + strand, within PS417_00325at 64.303 kb on + strandat 64.303 kb on + strandat 64.303 kb on + strandat 64.303 kb on + strandat 64.310 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
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61,517 - PS417_00305 0.77 +0.4
61,519 + PS417_00305 0.77 -1.3
61,520 - PS417_00305 0.77 -0.6
61,549 + PS417_00305 0.79 -0.3
61,550 - PS417_00305 0.79 +1.1
61,663 + PS417_00305 0.86 +0.6
61,994 - PS417_00310 0.17 +1.5
61,994 - PS417_00310 0.17 +0.2
62,151 - PS417_00310 0.45 +0.3
62,238 + PS417_00310 0.61 +2.0
62,239 - PS417_00310 0.61 +1.3
62,239 - PS417_00310 0.61 +0.8
62,264 - PS417_00310 0.66 +0.1
62,264 - PS417_00310 0.66 -0.2
62,264 - PS417_00310 0.66 +1.5
62,344 - PS417_00310 0.80 -0.2
62,449 + +0.3
62,453 + -0.1
62,505 + +0.8
62,505 + -0.0
62,505 + +0.4
62,506 - -0.4
62,508 + -3.0
62,537 - -0.3
62,537 - +0.3
62,537 - -1.7
62,661 + PS417_00315 0.20 +1.1
62,816 - PS417_00315 0.37 +0.6
62,931 + PS417_00315 0.50 +1.5
63,124 - PS417_00315 0.72 +0.3
63,124 - PS417_00315 0.72 -0.6
63,171 + PS417_00315 0.77 -0.2
63,172 - PS417_00315 0.77 +3.5
63,234 - PS417_00315 0.84 -0.1
63,364 - -0.1
63,364 - +0.4
63,364 - -0.4
63,624 + +0.2
63,624 + +2.3
63,625 - +0.6
63,625 - -0.3
63,625 - +1.8
63,625 - -2.5
63,625 - +0.2
63,627 - -0.3
63,638 + +0.1
63,638 + -0.7
63,639 - -1.4
63,760 + PS417_00325 0.19 +2.6
63,761 - PS417_00325 0.19 +0.6
63,761 - PS417_00325 0.19 -5.0
63,761 - PS417_00325 0.19 +0.5
63,761 - PS417_00325 0.19 +1.3
64,051 + PS417_00325 0.57 +1.4
64,052 - PS417_00325 0.57 +1.1
64,052 - PS417_00325 0.57 +0.5
64,174 + PS417_00325 0.73 +0.2
64,303 + -1.4
64,303 + +0.5
64,303 + -1.1
64,303 + +0.3
64,310 - -1.3

Or see this region's nucleotide sequence